Progression of Respiratory Syncytial Virus Infection Monitored by Fluorescent Quantum Dot Probes

Bentzen, Elizabeth L.; House, Frances S.; Utley, Thomas J.; Crowe, James E.; Wright, David W.

doi:10.1021/nl048073u

Cited by 98 publications

(75 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…1,146 This assay is useful if the functional group of the biomolecule being attached to the nanocrystal is an amine, which is often the case when coupling biomolecules to carboxylic acid terminated dots. The general strategy of the assay is to determine the number of free amines both before and after the conjugation, with the difference being the conjugation yield and the number of biomolecules on the surface.…”

Section: Characterizing the Bioconjugate Nanocrystal Surfacementioning

confidence: 99%

“…41 One such beautiful demonstration is that of Bentzen et al who used antibody conjugated core/shell nanocrystals to detect respiratory syncytial virus (RSV). 1 Respiratory infections are among the foremost causes of medical presentation in the United States and RSV is the leading cause of lower respiratory tract infections in infants and young children, resulting in an estimated 90,000 yearly hospitalizations of children under the age of five. There is an antiviral drug for RSV, but it is effective only when administered early in the infection.…”

Section: Extreme Brightness Yields Enhanced Sensitivity and Early Detmentioning

confidence: 99%

“…For example, antibody-conjugated CdSe/ ZnS core-shell nanocrystals have been used to detect respiratory syncytial virus. 1 The high brightness of the quantum dots enables the virus to be detected in a matter of minutes, whereas the previous assay required 4 days. The ramification of this new technology is that anti-viral drugs can be given early in the infection when they are most effective, reducing Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Synthesis, surface studies, composition and structural characterization of CdSe, core/shell and biologically active nanocrystals

Rosenthal

McBride

Pennycook

et al. 2007

Surface Science Reports

211

209

View full text Add to dashboard Cite

Nanostructures, with their very large surface to volume ratio and their non-planar geometry, present an important challenge to surface scientists. New issues arise as to surface characterization, quantification and interface formation. This review summarizes the current state of the art in the synthesis, composition, surface and interface control of CdSe nanocrystal systems, one of the most studied and useful nanostructures. KeywordsCadmium; Selenium; Selenide; CdSe; nanocrystal; quantum dot; nanoparticle; surface; synthesis; electron microscopy; Z-STEM; RBS; alloy; core/shell; fluorescence; quantum yield; growth mechanism; expitaxy; spectroscopy 1.0 Introduction BackgroundWe are currently witnessing an explosion of research in nanoscale science, engineering, and biotechnology. As the nano-revolution proceeds, basic research is leading to the development of true nanotechnologies which have the potential to impact fields from health care and opto-electronics to energy and the environment. One particular type of nanostructure, semiconductor nanocrystals (also known as quantum dots), may ultimately be employed in technologies in each of these fields. For example, antibody-conjugated CdSe/ ZnS core-shell nanocrystals have been used to detect respiratory syncytial virus. 1 The high brightness of the quantum dots enables the virus to be detected in a matter of minutes, whereas the previous assay required 4 days. The ramification of this new technology is that anti-viral drugs can be given early in the infection when they are most effective, reducing Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. NIH Public Access Author ManuscriptSurf Sci Rep. Author manuscript; available in PMC 2011 April 6. NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author Manuscript hospitalizations and deaths. In the field of opto-electronics CdSe nanocrystals have been incorporated in light emitting diodes2 -6 and it has been demonstrated that they can serve as the gain media in a laser.7 , 8 Nanocrystals are also poised to play a role in solar energy conversion.9 -14 A specific example is a device in which elongated CdSe nanocrystals (nanorods) are mixed with a conducting polymer and sandwiched between aluminum and indium tin oxide electrodes to create a device with a power conversion efficiency of 1.7 %. 12 Nanocrystals may also have a positive impact on the environment, as it has been shown that they can be used to sequester CO 2 . 15 The spark that ignited two decades of intense research in nanocrystal synthesis, properties, and applications was Louis Brus' demonstration that qua...

show abstract

Section: Characterizing the Bioconjugate Nanocrystal Surfacementioning

confidence: 99%

Section: Extreme Brightness Yields Enhanced Sensitivity and Early Detmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Synthesis, surface studies, composition and structural characterization of CdSe, core/shell and biologically active nanocrystals

Rosenthal

McBride

Pennycook

et al. 2007

Surface Science Reports

211

209

View full text Add to dashboard Cite

show abstract

“…A QD system can detect the presence of particles of the RSV in a matter of hours. It is also more sensitive, allowing detection of the virus earlier in the course of an infection (16 ). When an RSV virus infects lung cells, it leaves part of its coat containing F and G proteins on the cell's surface.…”

Section: Qdsmentioning

confidence: 99%

Applications of Nanobiotechnology in Clinical Diagnostics

2007

View full text Add to dashboard Cite

Background: Nanobiotechnologies are being applied to molecular diagnostics and several technologies are in development. Methods: This review describes nanobiotechnologies that are already incorporated in molecular diagnostics or have potential applications in clinical diagnosis. Selected promising technologies from published literature as well as some technologies that are in commercial development but have not been reported are included. Results: Nanotechnologies enable diagnosis at the single-cell and molecule levels, and some can be incorporated in current molecular diagnostic methods, such as biochips. Nanoparticles, such as gold nanoparticles and quantum dots, are the most widely used, but various other nanotechnological devices for manipulation at the nanoscale as well as nanobiosensors are also promising for potential clinical applications. Conclusions: Nanotechnologies will extend the limits of current molecular diagnostics and enable point-ofcare diagnostics, integration of diagnostics with therapeutics, and development of personalized medicine. Although the potential diagnostic applications are unlimited, the most important current applications are foreseen in the areas of biomarker discovery, cancer diagnosis, and detection of infectious microorganisms. Safety studies are needed for in vivo use. Because of its close interrelationships with other technologies, nanobiotechnology in clinical diagnosis will play an important role in the development of nanomedicine in the future.

show abstract

“…Antibodies are widely used to detect viruses and viral proteins (Cho et al 1996;Bentzen et al 2005;Dawson 2007;Rabenau et al 2007;Polage and Petti 2009), but due to their specificity, must be produced and calibrated for every target and are highly vulnerable to viral mutations. Quantitative reverse-transcription polymerase chain reaction (qRT-PCR), microarrays, and enzymelinked immunosorbent assays (ELISAs) are other widely used methods to detect and quantify viruses (Steininger et al 2002;Wang et al 2002;Fox 2007;Leski et al 2009;Mosleh et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Monitoring viral RNA in infected cells with LNA flow-FISH

Robertson

Verhoeven²,

Thach³

et al. 2010

RNA

View full text Add to dashboard Cite

We previously showed the feasibility of using locked nucleic acid (LNA) for flow cytometric-fluorescence in situ hybridization (LNA flow-FISH) detection of a target cellular mRNA. Here we demonstrate how the method can be used to monitor viral RNA in infected cells. We compared the results of the LNA flow-FISH with other methods of quantifying virus replication, including the use of an enhanced green fluorescent protein (EGFP) viral construct and quantitative reverse-transcription polymerase chain reaction. We found that an LNA probe complementary to Sindbis virus RNA is able to track the increase in viral RNA over time in early infection. In addition, this method is comparable to the EGFP construct in sensitivity, with both peaking around 3 h and at the same level of infected cells. Finally, we observed that the LNA flow-FISH method responds to the decrease in levels of viral RNA caused by antiviral medication. This technique represents a straightforward way to monitor viral infection in cells and is easily applicable to any virus.

show abstract

Progression of Respiratory Syncytial Virus Infection Monitored by Fluorescent Quantum Dot Probes

Cited by 98 publications

References 16 publications

Synthesis, surface studies, composition and structural characterization of CdSe, core/shell and biologically active nanocrystals

Synthesis, surface studies, composition and structural characterization of CdSe, core/shell and biologically active nanocrystals

Applications of Nanobiotechnology in Clinical Diagnostics

Monitoring viral RNA in infected cells with LNA flow-FISH

Contact Info

Product

Resources

About