Progressive multifocal leucoencephalopathy: Analysis of JC virus DNA from brain and kidney tissue

Viral variant nucleotide sequences help expose leukocytic positioning in the JC virus pathway to the CNS

“…As previously cited, initial reports of regulatory region variation came from genomic JCV sequences derived mainly from cultured isolates of PML-brain tissue [18,26,34].…”

“…Polyomaviruses were, therefore, distinguishable by nucleic acid cohybridization techniques such as Southern blotting [25][26][27] and in situ hybridization [28,29]. JCV remains the only virus consistently detected in PML brain tissue, establishing it as the etiologic agent.…”

Distribution of JC Virus DNA in Peripheral Blood Lymphocytes of Hematological Disease Cases.

“…1) isolated from the brain of a PMLautopsy case shows a base sequence different from that of the JCV genome isolated from the kidney of healthy subjects (8,9). It has been suggested that this structural alternation is the cause of PML onset (10).…”

Two Major Types of JC Virus Defined in Progressive Multifocal Leukoencephalopathy Brain by Early and Late Coding Region DNA Sequences

“…The many efforts to type JCV isolates by DNA sequence variation have focused on the non-coding regulatory region. The hypervariable regulatory region of virus isolated from PML brain has multiple sequence rearrangements, with extensive deletions and duplication, which vary with each isolate (GrinneU et al, 1983b;Martin et al, 1985;D6rries, 1984;Loeber & D6rries, 1988;Walker & Frisque, 1986;Major et al, 1992). Classification schemes based on the structure of the regulatory region have been described (Martin et al, 1985;Walker & Frisque, 1986), but it is not clear whether the patterns of sequence rearrangement reflect the genotype of the virus or result from host cell biology.…”