Progressive Replacement of Human Cultured Epithelial Allografts by Recipient Cells as Evidenced by HLA Class I Antigens Expression

Gielen, V.; Faure, Michel; Mauduit, G; Thivolet, J

doi:10.1159/000248820

Cited by 72 publications

(26 citation statements)

References 11 publications

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“…In contrast to allografts of split-thickness transplantation for xenografts. In the allograft model, CD3+ cell infiltration was significantly higher in neonaskin, rejection of CSS does not appear to be an acute process, but rather occurs via an ill-defined process observation is consistent with other reports that have shown the loss of class II expressing Langerhans and where allogeneic cells are lost over time with simultaneous replacement by autologous cells (11). Critical to undendritic cells during the culture of human keratinocytes (15,21).…”

Section: Introductionsupporting

confidence: 87%

Allogeneic versus Xenogeneic Immune Reaction to Bioengineered Skin Grafts

Erdag

Morgan

2004

Cell Transplant

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There are conflicting reports on the survival and immune reaction to allografts and xenografts of cultured skin substitutes (CSS). In this study, we investigated the allogeneic and xenogeneic responses to CSS of human keratinocytes and genetically engineered CSS expressing keratinocyte growth factor (KGF) that forms a hyperproliferative epidermis. CSS (control and KGF modified) and neonatal human foreskins were evaluated by immunohistochemistry for the expression of MHC class I and II. To study allograft rejection, grafts were transplanted to human peripheral blood mononuclear cell (huPBMC)-reconstituted SCID mice. To study xenograft rejection, grafts were transplanted to immunocompetent mice. Graft survival and immune reaction were assessed visually and microscopically. After transplantation, control CSS formed a normal differentiated epidermis, whereas KGF CSS formed a hyperproliferative epidermis. Control and KGF CSS expressed class I similar to neonatal foreskin, but did not express class II. In the allograft model, rejection of neonatal foreskins was between 5 and 9 days. In contrast, neither control nor KGF CSS was rejected by huPBMC-SCID mice. Histology showed dense mononuclear cell infiltration in human foreskins, with few, if any, mononuclear cells in control or KGF CSS. In contrast to the allogeneic reaction, CSS (control and KGF) were rejected in the xenograft model, but rejection was delayed (9-21 days) compared with neonatal skin (5-8 days). Humanized SCID mice rejected allografts of human neonatal foreskins, but did not reject control CSS or KGF CSS, even though the KGF CSS formed a hyperproliferative epidermis. Rejection of control and KGF CSS by immunocompetent mice in a xenograft model was comparable and their survival was significantly prolonged compared with neonatal skin. These results demonstrate that control CSS and hyperproliferative KGF CSS are less immunogenic than normal human skin and that sustained hyperproliferation of the epidermis does not accelerate rejection.

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Section: Introductionsupporting

confidence: 87%

Allogeneic versus Xenogeneic Immune Reaction to Bioengineered Skin Grafts

Erdag

Morgan

2004

Cell Transplant

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“…Although the clinical observations favored this hypothesis, various techniques have failed to demonstrate long-term survival of cultured keratinocyte allografts in humans (Burt et al, 1989;De Luca et al, 1989). Apparently these grafts are not rejected, but are rather gradually replaced with recipient cells (Gielen et al, 1987 (Lerner et al, 1987). The various methods for in vitro engineering of the epidermis that have evolved until now characteristically require:…”

Section: Introductionmentioning

confidence: 99%

Tissue Engineering of Skin

Pomahac¹,

Svensjö²,

Yao³

et al. 1998

Critical Reviews in Oral Biology & Medicine

103

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The skin plays a crucial role in protecting the integrity of the body's internal milieu. The loss of this largest organ is incompatible with sustained life. In reconstructive surgery or burn management, substitution of the skin is often necessary. In addition to traditional approaches such as split-or full-thickness skin grafts, tissue flaps and free-tissue transfers, skin bioengineering in vitro or in vivo has been developing over the past decades. It applies the principles and methods of both engineering and life sciences toward the development of substitutes to restore and maintain skin structure and function. Currently, these methods are valuable alternatives or complements to other techniques in reconstructive surgery. This review article deals with the evolution and current approaches to the development of in vitro and in vivo epidermis and dermis.

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“…Initially, it was believed that this phenomenon was due to the relative immunodepression of the burn patient and to the loss of class II histocompati bility antigens in the cultured epidermis. Nowadays, it is generally accepted that the allogenic epidermal cells are gradually replaced by autologous keratinocytes of the recipient [7], The use of cultured epidermal cells in the treatment of chronic leg ulcers was reported for the first time in 1986 [8,9].…”

Section: Introductionmentioning

confidence: 99%

Repeated Cultured Epidermal Allografts in the Treatment of Chronic Leg Ulcers of Various Origins

Beele¹,

Naeyaert²,

Goeteyn³

et al. 1991

Dermatology

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Twelve patients with 16 leg ulcers, existing for at least 3 months and not responsive to conventional inpatient therapy of at least 3 weeks, were treated with repeated applications of cultured allogenic keratinocyte sheets. A marked decrease in size was seen in all ulcers but 2. Complete closure of the ulcer was seen in 62% of the ulcers within 8 weeks. Healing was due to enhanced granulation and increased epithelialization, starting from the periphery of the wound. This edge effect suggests that the epidermal allografts act by stimulation of migration and/or multiplication of the acceptor’s keratinocytes, rather than by take of the allograft.

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Progressive Replacement of Human Cultured Epithelial Allografts by Recipient Cells as Evidenced by HLA Class I Antigens Expression

Cited by 72 publications

References 11 publications

Allogeneic versus Xenogeneic Immune Reaction to Bioengineered Skin Grafts

Allogeneic versus Xenogeneic Immune Reaction to Bioengineered Skin Grafts

Tissue Engineering of Skin

Repeated Cultured Epidermal Allografts in the Treatment of Chronic Leg Ulcers of Various Origins

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