Prohormone convertases PC2 and PC3 in rat neutrophils and macrophages

Vindrola, Osvaldo; Mayer, Alejandro M. S.; Citera, Gustavo; Spitzer, Judy A.; Lr, Espinoza

doi:10.1016/0143-4179(94)90004-3

Cited by 42 publications

(21 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We focused on PENK since previous studies have shown its expression in immune cells, including neutrophils, lymphocytes and macrophages (12)(13)(14)19). Furthermore, recent studies have also demonstrated that antibacterial activities could be derived from the C-terminal processing of PENK (23).…”

Section: Lps-induction Of Proenkephalin In the Spleen -mentioning

confidence: 99%

“…PC1/3 expression has been shown in a human monocyte cell line (25) or in differentiated macrophages (13,25) whereas PC2 was detected in polymorphonuclear leukocytes (13). Recently, PC1/3 and PC2 expression have been demonstrated in rat immunocytes of the blood and within inflamed tissue (26).…”

mentioning

confidence: 99%

“…However, it is now known that expression of various neuropeptide precursors, such as proopiomelanocortin (POMC) (10,11), proenkephalin (PENK) (12)(13)(14) and endomorphins (15), known to be processed by PCs, is not limited to the neuroendocrine system, and has been demonstrated in various cells of the immune system. Mature forms of the expected neuropeptides have been isolated and characterized in immunocytes, such as peptides with immunoregulatory and signaling functions (16)(17)(18).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Lipopolysaccharide mediated regulation of neuroendocrine associated proprotein convertases and neuropeptide precursor processing in the rat spleen

Lansac

Dong

Dubois

et al. 2006

Journal of Neuroimmunology

View full text Add to dashboard Cite

Section: Lps-induction Of Proenkephalin In the Spleen -mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Lipopolysaccharide mediated regulation of neuroendocrine associated proprotein convertases and neuropeptide precursor processing in the rat spleen

Lansac

Dong

Dubois

et al. 2006

Journal of Neuroimmunology

View full text Add to dashboard Cite

“…Fax: (1) (773) 702-4292. E-mail: jlamendo@midway.uchicago.edu alveolar and splenic macrophages [9] led us to hypothesize the existence of these enzymes in human monocyte-derived macrophages (HMDM) and in the human monocytic leukemia cell line THP-1 as well. Accordingly, we have investigated the expression of PC3, PC2, CPE and enkephalin (ENK) mRNAs by RT-PCR in HMDMs and in THP-1 cells and have examined whether these enzymes are induced during normal human monocytic differentiation in vitro.…”

Section: Introductionmentioning

confidence: 99%

Expression of PC3, carboxypeptidase E and enkephalin in human monocyte‐derived macrophages as a tool for genetic studies

1997

View full text Add to dashboard Cite

Circulating monocytes in human peripheral blood are readily available, easily obtained, and can be cultured in vitro. Once plated, the monocytes spontaneously differentiate into macrophages. Undifferentiated human monocytes do not express carboxypeptidase E (CPE), prohormone convertase 3 (PC3/ PCI) or proenkephalin (ENK), suggesting that gene induction during differentiation results in the expression of these genes. RT-PCR of human monocyte-derived macrophage (HMDM) mRNA showed detectable levels of ENK mRNA at 48 h after plating, followed by PC3 and CPE mRNAs at 72 h.

show abstract

“…All antisera were dissolved in blocking solution containing 0.05% Tween 20. PC1 antibody ‘2B6’ and PC2 antibody ‘Thumpa’ have been previously characterized and utilized [33, 34, 35, 36, 37]. …”

Section: Methodsmentioning

confidence: 99%

Modulation of Prohormone Convertase 2 in Spinal Cord during Gestation and Hormone-Simulated Pregnancy

Varshney

Rivera²,

Gintzler³

1999

Neuroendocrinology

View full text Add to dashboard Cite

Gestation as well as its hormonal simulation (HSP) is characterized by an enhanced spinal dynorphin/ĸ-opioid antinociception. This antinociception is accompanied by decreased content of dynorphin precursor intermediates and increased content of mature dynorphin peptides (1–17 and 1–8) in the lumbar spinal region. This suggests that augmented processing of spinal dynorphin precursor intermediates is an adaptive mechanism used by dynorphin neurons to meet increased synthetic demands necessitated by increased dynorphin neurotransmission. Prohormone convertase (PC) 1 and 2 represent major secretory granule proteolytic processing activities capable of converting neuroendocrine and neurotransmitter peptide (dynorphin) precursor intermediates to their mature, biologically active products. Accordingly, the current investigation was undertaken to assess their potential relevance to peptidergic (dynorphin) neuronal functional plasticity in vivo. In order to evaluate a molecular biological parameter of PC2 synthesis, a solution hybridization assay was developed with which to quantify changes in the spinal lumbar content of its mRNA. This study demonstrates that during gestation and HSP, lumbar PC2 protein content, but not that of PC1, is augmented. The increase in lumbar PC2 during HSP indicates that the pregnancy blood concentration profile of 17β-estradiol and progesterone is a predominant facet of the pregnant condition responsible for its modulation during this condition. In contrast to the elevated content of lumbar PC2 protein, levels of PC2 mRNA in the lumbar cord of pregnant or HSP rats were essentially unchanged. This indicates that increased transcriptional activity is not, necessarily, a prerequisite for increased PC2 protein content to be manifest. These observations suggest positive modulation of PC2 to be a critical component of the mechanism(s) by which spinal dynorphin neurons adapt to the demand-induced increased production of mature dynorphin peptides.

show abstract

Prohormone convertases PC2 and PC3 in rat neutrophils and macrophages

Cited by 42 publications

References 41 publications

Lipopolysaccharide mediated regulation of neuroendocrine associated proprotein convertases and neuropeptide precursor processing in the rat spleen

Lipopolysaccharide mediated regulation of neuroendocrine associated proprotein convertases and neuropeptide precursor processing in the rat spleen

Expression of PC3, carboxypeptidase E and enkephalin in human monocyte‐derived macrophages as a tool for genetic studies

Modulation of Prohormone Convertase 2 in Spinal Cord during Gestation and Hormone-Simulated Pregnancy

Contact Info

Product

Resources

About