Proinflammatory and Anti-inflammatory Cytokines in Preterm Neonates with Acute Respiratory Distress Syndrome

Перепелица, С. А.; Голубев, А. М; Мороз, В. В.; Алексеева, С. В.; Буева, Ж. В.; Редина, Н. В.; Шульга, Р. А.; Салазкина, Т. А.; Леонтюк, Н. В.

doi:10.15360/1813-9779-2009-6-21

Cited by 3 publications

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References 5 publications

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“…Различие между показате лями статистически значимое (p<0,001). Выделение подгрупп важно для понимания процессов постнаталь ной иммунной адаптации, т. к. в раннем неонатальном периоде динамика цитокинов у новорожденных разнона правленная [18,19]. Содержание ИЛ 6 в ЖА и бронхо альвеолярном аспирате (БАЛ) достоверно не отличалось между подгруппами (p>0,05).…”

Section: результаты и обсуждениеunclassified

Placental Inflammatory Changes and Bacterial Infection in Premature Neonates with Respiratory Failure

Перепелица¹,

Голубев²,

Мороз³

et al. 2012

rmt

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Цель исследования-выявить взаимосвязь воспалительных изменений в плаценте с бактериальной инфекцией у недо ношенных новорожденных с дыхательной недостаточностью. Материал и методы. У 157 недоношенных новорожден ных с респираторным дистресс синдромом (РДСН) проведено бактериологическое исследование бронхоальвеолярно го аспирата, в периферической крови определено общее количество лейкоцитов и выполнен дифференциальный подсчет лейкоцитов. В различных биологических жидкостях матерей и детей исследовано содержание цитокинов ИЛ 1β β, ИЛ 4, ИЛ 6 и TNF α α, а также выполнено морфологическое исследование плаценты. Результаты. При анализе бакте риологических посевов из трахеобронхиального дерева в 61,8% случаев роста бактериальной микрофлоры не получе но, у 6,4% детей выделен Enterococcus faecalis, в 8,3% случаев-Staphylococcus epidermidis, в 1,9% случаях-Staphylococcus haemolyticus, Staphylococcus capitis, Enterobacter agglomerans, гемолитический стрептококк группы А, кроме того, у 1,3% новорожденных выделены Bacillus spp., Staphylococcus aureus, Escherichia coli, Acinetobacter spp., Serratia marcescens. Другие микроорганизмы и микробная ассоциация встречались в 8,9% случаев. При морфологиче ском исследовании плаценты выявлены различные воспалительные изменения в сочетании с хронической и острой пла центарной недостаточностью. Исследование показало, что в плазме периферической крови матерей в конце первого периода родов содержание ИЛ 1β β, ИЛ 4, ИЛ 6, TNF α α находилось в пределах физиологических значений. В амниоти ческой жидкости определяли повышенное содержание ИЛ 6 и TNF α α при нормальном уровне ИЛ 4 и ИЛ 1β β, что свиде тельствовало о внутриматочном воспалительном процессе. Заключение. Преждевременные роды ассоциированы с различными воспалительными изменениями в плаценте, что вызывает внутриутробную стимуляцию макрофагов вор син хориона. Формируются специфические иммунологические механизмы защиты, которые препятствуют развитию инфекционного процесса у плода. Т. е. наличие у матери инфекционного процесса может быть причиной преждевре менных родов, но не приводить к заболеванию плода и новорожденного. Исследование показало, что основным забо леванием у недоношенных новорожденных является РДСН. Ключевые слова: внутриутробная инфекция, недоношен ные новорожденные, цитокины, респираторный дистресс синдром новорожденных. Objective: to reveal a relationship of placental inflammatory changes to bacterial infection in premature neonates with respiratory failure. Material and methods. Bronchoalveolar aspirate was bacteriologically studied in 157 pre mature neonates with respiratory distress syndrome (NRDS); the total and differential leukocyte counts were mea sured in their peripheral blood. The levels of the cytokines IL 1β β, IL 4, IL 6, and TNF α α were studied in different biological fluids of mothers and their babies; the placentas were also morphologically examined. Results. An analy sis of bacterial cultures from the tracheobronchial tree revealed no growth of bacterial microflora in 61.8% of cases, Enterococcus faecal...

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Section: результаты и обсуждениеunclassified

Placental Inflammatory Changes and Bacterial Infection in Premature Neonates with Respiratory Failure

Перепелица¹,

Голубев²,

Мороз³

et al. 2012

rmt

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Effects of intravenous immunoglobulin on the negatively transformed subpopulations of neutrophilic granulocytes in newborns with congenital pneumonia and neonatal sepsis

Nesterova,

Kovaleva,

Chudilova

et al. 2023

Med. immunol.

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Today, the diagnosis and treatment of severe infectious and inflammatory diseases in newborns, e.g., congenital pneumonia (CP) and neonatal sepsis (NS), present difficult problems. Searching sensitive and specific severity markers of bacterial inflammatory process as well as early and effective treatment are crucial for the outcome and prognosis of these life-threatening diseases. The aim of our study was to assess the effects of intravenous immunoglobulin (IVIG) injections on the negatively transformed subpopulations of neutrophilic granulocytes (NG) СD64-CD16+СD32+СD11b+, СD64+CD16+СD32+СD11b+ and evaluation of their functional activity in newborns with CP and NS. We have observed 38 full-term newborn patients. Group 1 included 19 infants with CP, including 11 children who received conventional therapy and IVIG (group 1.1), and 8 children treated at conventional protocols (group 1.2). Group 2 included 19 children with NS, including 12 children who underwent conventional therapy and IVIG treatment (group 2.1), and 7 children who were subject to conventional therapy (group 2.2). The comparison group consisted of 22 healthy full-term newborns. Testing of NG population included the following parameters: counting the numbers of NG subpopulations which simultaneously expressed CD11b CD64, CD32, CD16, as well as their phenotypic patterns, with regard of the receptor expression density (MFI) using flow cytometric techniques. Moreover, we determined phagocytic and microbicidal activity of the granulocytes. We have revealed negative transformation of СD64-CD16+СD32+СD11b+ and СD64+CD16+СD32+СD11b+ subpopulations of neutrophilic granulocytes in newborns with CP and NS, The diagnostic significance of increased СD64+CD16+СD32+СD11b+NG subpopulation was more pronounced with increasing severity of bacterial infection and inflammatory process, i.e., 18.7-fold in CP, 52.3-fold in NS, along with predominant decrease in expression of appropriate membrane receptors. These phenotypic changes were associated with impaired phagocytic and killing activity of NG. The effect of IVIG on the impaired mechanisms of antibacterial immunity is associated not only with alleviation of IgG deficiency, but also with positive remodeling of negatively transformed subpopulations of СD64-CD16+СD32+СD11b+NG and СD64+CD16+СD32+СD11b+NG, improved effector functions of NG, especially in cases of CP. Thus, following IVIG treatment, a reduced number of СD64-CD16+СD32+СD11b+NG subpopulations was fully recovered in CP, while it increased 1.5 times in NS, and the content of diagnostically significant СD64+CD16+СD32+СD11b+NG subpopulation showed a significantly decrease, both in CP (2-fold) and in NS (2.6-fold). However, this index remained higher than the content of this subpopulation in healthy newborns. At the same time, we have noted the restorative or modulatory effects by changing density of trigger molecules in NG subpopulations. Limitation of the negative NG transformation in their functionally significant subpopulations in newborns with CAP and NS was accompanied by positive clinical effects, i.e., optimization of antibiotic therapy, reduced duration of treatment, and improved mortality rates.

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The influence of cytokine reactions on the nature of the course of adaptive mechanisms in newborns of different gestational ages

Vakhitov,

Loskutova,

Vakhitova

et al. 2023

Medicinskij sovet

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Introduction. The study of the mechanisms of adaptation and consequences of deadaptation in newborns, and especially premature babies in the first days of life is an integral part of neonatal practice. The cytokine system is considered as sensitive and informative indicators of homeostatic disorders in newborns of different gestational ages.Aim. To assess the features of the early neonatal period in newborns of different gestational ages according to the nature of changes in the levels of interleukin-1, -4, -8 in the cord blood and on the 3-4th day of the infant's life.Materials and methods. 108 newborns were examined, of which 64 premature infants of the gestational age of 32 (0/7) - 36 (6/7) weeks were included in the treatment group: moderately premature infants (32 (0/7) - 33 (6/7) weeks) and late premature infants (34 (0/7) - 36 (6/7) weeks). The control group included 44 conditionally healthy full-term infants born between 37 (0/7) and 41 (6/7) week of gestation: early full-term (37 (0/7) - 38 (6/7) weeks) and full-term intants (more than 39 (0/7) weeks).Results and discussion. The full-term infants, as well as late premature infants, whose clinical status was assessed as satisfactory, usually showed minimum levels of all studied cytokines. If the early neonatal period was complicated, high levels of interleukin-1 and -8 with underlying slightly elevated levels of anti-inflammatory interleukin-4 were observed. This fact supports the activation of cytokine reactions during manifestation of pathological symptom groups generated by various organs and systems in the early neonatal period.Conclusion. The nature of cytokine reactions can serve as a marker of unfavourable course of the early neonatal period in premature infants and a criterion that justifies the implementation of necessary corrective measures.

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Proinflammatory and Anti-inflammatory Cytokines in Preterm Neonates with Acute Respiratory Distress Syndrome

Cited by 3 publications

References 5 publications

Placental Inflammatory Changes and Bacterial Infection in Premature Neonates with Respiratory Failure

Placental Inflammatory Changes and Bacterial Infection in Premature Neonates with Respiratory Failure

Effects of intravenous immunoglobulin on the negatively transformed subpopulations of neutrophilic granulocytes in newborns with congenital pneumonia and neonatal sepsis

The influence of cytokine reactions on the nature of the course of adaptive mechanisms in newborns of different gestational ages

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