Proinflammatory Cytokine Single Nucleotide Polymorphisms in Nasal Polyposis

Erbek, Selim S.; Yurtçu, Erkan; Erbek, Seyra; Ataç, Fatma Belgin; Şahin, Feride İffet; Cakmak, Ozcan

doi:10.1001/archotol.133.7.705

Cited by 73 publications

(75 citation statements)

References 22 publications

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“…Polyp tissue includes mixed inflammatory cells, of which eosinophils are the most dominant. They have the primary role in the perpetuation of chronic inflammation (2,3). How ever, polyp tissue eosinophilia is an entity independent of atopy (3).…”

Section: Introductionmentioning

confidence: 99%

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Cytokine Profiles in Nasal Fluid in Patients with Nasal Polyps: A Flow Cytometric Study

Perić¹,

Vojvodić²,

Radulović³

2010

Journal of Medical Biochemistry

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Cytokine Profiles in Nasal Fluid in Patients with Nasal Polyps: A Flow Cytometric StudyBiological markers in nasal fluid provide valuable information on nasal pathophysiology. The aims of this study were to compare the cytokine profiles of nasal fluid in subjects with nasal polyps (NP) and co-morbid asthma and NP patients without asthma and to determine the role of these cytokines in the development of NP. Thirty patients with NP (15 asthmatic and 15 non asthmatic) were included in this prospective study. Nasal secretion samples were collected from nasal cavities of all 30 subjects. The levels of eleven cytokines (TNF-α, TNF-β, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, and IFN-γ) were measured using flow cytometry. The concentrations of Th2 cytokines IL-5, IL-6 and IL-10 were significantly higher in patients with NP and asthma compared with subjects with NP without asthma. We also found significantly higher levels of IFN-α, IL-4, IL-6 and IL-10 in allergic patients with NP and asthma compared with those without asthma. In nonallergic patients with NP and asthma, the concentrations of TNF-α, IL-5 and IL-6 were significantly higher than in nonallergic patients with NP without asthma. Our results show that the presence of Th2 cytokines, especially IL-5 and IL-6 in patients with NP and asthma is a more prominent feature than in those without asthma that relates to the increased eosinophilic inflammation. We have also found a significant influence of allergy on the cytokine profiles both in asthmatic and nonasthmatic patients.

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Section: Introductionmentioning

confidence: 99%

“…They have the primary role in the perpetuation of chronic inflammation (2,3). How ever, polyp tissue eosinophilia is an entity independent of atopy (3). In non-atopic asthma, polyps are diagnosed more frequently, in about 10-15% patients (2).…”

Section: Introductionmentioning

confidence: 99%

Cytokine Profiles in Nasal Fluid in Patients with Nasal Polyps: A Flow Cytometric Study

Perić¹,

Vojvodić²,

Radulović³

2010

Journal of Medical Biochemistry

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“…In the North America region, Randolph et al (21) found that TNF-α gene promoter region allele frequency did not affect asthma formation, whereas Witte et al (22) found the A allele to be a risk factor. The different results in the Turkish population (7,8, and our study) can be explained by genetic variations in different regions of the country, patient selection criteria, and differences in study design. Moreover, the effect of allelic frequencies and genotypes of the TNF-α gene promoter region on the transcription of TNF-α is not clear.…”

Section: Discussionmentioning

confidence: 54%

“…Single nucleotide polymorphisms (SNPs) in these pro-inflammatory mediators have been studied for genetic susceptibility to NP development, but the literature findings regarding the dominant genotype or allele causing NP formation are conflicting. Some authors claim that the TNF-α GA polymorphism in the -308 promoter region is a risk factor for NP development, whereas others have found that the A allele frequency is a risk factor (7)(8)(9). Some authors have found no relationship between the TNF-α GA polymorphism and NP development (4,10).…”

Section: Introductionmentioning

confidence: 99%

TNF-α and IL-1 β Cytokine Gene Polymorphism in Patients with Nasal Polyposis

İsmi¹,

Özcan²,

Polat³

et al. 2017

Turk Arch Otorhinolaryngol

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Objective: Nasal Polyp (NP) is a benign mass of the paranasal sinuses that protrudes into the nasal cavity. The exact underlying pathogenesis is not known. In this study we aimed to determine the genetic susceptibility of NP formation in relation to TNF-α-308 and IL-1β-511 promoter region gene polymorphisms. Methods:A total of 71 patients with NP with asthma (n=21) or without asthma (n=50) were taken as the study group, and 91 healthy volunteers were taken as the control group. Blood was gathered into EDTA-containing tubes, and patient DNA was extracted. The polymorphisms of the IL-β and TNF-α cytokine genes were analyzed using real time polymerase chain reaction. Results:The GG genotype in the TNF-α-308 region and the CC genotype in the IL-1β-511 region were found to be risk factors for NP formation (OR: 9.2, p=0.007 and OR: 33.3, p=0.001, respectively). Regarding allelic frequencies, the G allele at the TNF-α-308 promoter region was a risk factor for NP formation (OR: 6.06, p<0.001).Conclusion: TNF-α GG genotype in the -308 promoter region and the IL-1β CC genotype in the -511 region are genetic risk factors for NP formation.

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“…Polyp tissue includes mixed inflammatory cells, of which eosinophils are the most dominant. They have the primary role in the perpetuation of chronic inflammation [2,3]. It has been suggested that an ineffective local Th1-based immune response in these patients is associated with increased Th2-cytokine-based activity, which contributes to a chronic infection as well as to an increased presence of eosinophils, which then lead to further polyp formation [4].…”

Section: Introductionmentioning

confidence: 99%

Correlation Between Cytokine Levels in Nasal Fluid and Scored Clinical Parameters in Patients with Nasal Polyposis

Perić

Vojvodić

Radulović

et al. 2012

Indian J Otolaryngol Head Neck Surg

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The aim of this study was to compare the cytokine levels in nasal fluid in subjects with nasal polyposis (NP) and co-morbid asthma and NP patients without asthma and to correlate these levels with clinical parameters of severity of disease. Forty NP patients (20 asthmatic and 20 nonasthmatic) were enrolled. Nasal secretion samples were collected from nasal cavities of all 40 subjects. The levels of Th1 cytokines IL-2, IL-12 and IFN-c, Th2 cytokines IL-4, IL-5, IL-6, and IL-10, chemokine IL-8, and proinflammatory cytokines IL-1b, TNF-a and TNF-b were measured using flow cytometric method. Each of the 40 patients was staged clinically according to global nasal symptom score, endoscopic score, and Lund-Mackay computed tomography (CT) score. The concentrations of Th2 cytokines IL-5, IL-6 and IL-10 were significantly higher (P \ 0.01, P \ 0.01, P \ 0.05) in patients with NP and asthma compared with NP patients without asthma.Positive correlations were observed between concentration of IL-2 in nasal secretions and global nasal symptom score, endoscopic score, and Lund-Mackay score only in NP patients without asthma. We also found positive correlation between CT score and the levels of IL-8, IL-4, and IL-1b in nonasthmatic patients. Finally, our results showed a positive correlation between IL-5 levels in nasal fluid and endoscopic score only in asthmatic patients. NP in asthmatic patients have different immunological patterns compared to those without asthma. We also concluded that concentrations of cytokines measured in nasal fluid were not sensitive enough to determine the severity of disease.

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Proinflammatory Cytokine Single Nucleotide Polymorphisms in Nasal Polyposis

Abstract: To investigate the association between nasal polyposis (NP) and single nucleotide polymorphisms of the proinflammatory cytokines IL (interleukin) 1␣ (the IL1A gene), IL-1␤ (the IL1B gene), and tumor necrosis factor ␣ (the TNFA gene).

Cited by 73 publications

References 22 publications

Cytokine Profiles in Nasal Fluid in Patients with Nasal Polyps: A Flow Cytometric Study

Cytokine Profiles in Nasal Fluid in Patients with Nasal Polyps: A Flow Cytometric Study

TNF-α and IL-1 β Cytokine Gene Polymorphism in Patients with Nasal Polyposis

Correlation Between Cytokine Levels in Nasal Fluid and Scored Clinical Parameters in Patients with Nasal Polyposis

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