Proinflammatory Event of Ozonized Olive Oil in Mice

Kataoka, Hiromi; Semma, Masanori; Sakazaki, Humitoshi; Nakamuro, Katsuhiko; Yamamoto, Tadashi; Hirota, Seiichi; Tazuya-Murayama, Keiko; Ichikawa, Atsushi

doi:10.1080/01919510902839022

Cited by 12 publications

(11 citation statements)

References 15 publications

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“…This approach is justified because the FT-IR technique is less specific than 1 H NMR when small differences in chemical structure are present. 1 H NMR data showed the reaction of ozone with only the >C --C< corresponding to fatty acids. The chemical shifts observed in this technique were barely influenced by neighboring moieties.…”

Section: Ft-ir and 1 H Nmr Spectroscopic Analysismentioning

confidence: 99%

“…The authors have found that ozonated olive oil produces both swelling‐suppressive and proinflammatory effects. However, its repeated application markedly induces the swelling reaction . These results reveal the need to develop control methods for ozonated oil production.…”

Section: Introductionmentioning

confidence: 97%

“…Ozonated vegetable oils have many interesting applications in the food, cosmetic, and pharmaceutical industries, as well as in medicine. In fact, in some European countries, such as Germany, they can be obtained on prescription from pharmacies . Countries such as Cuba have developed commercial ozonated oils, and they have been successfully tested for the treatment of many diseases .…”

Section: Introductionmentioning

confidence: 99%

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Correlation of structural characterization and viscosity measurements with total unsaturation: An effective method for controlling ozonation in the preparation of ozonated grape seed and sunflower oils

Guerra-Blanco¹,

Poznyak²,

Chairez³

et al. 2015

Euro J Lipid Sci & Tech

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Ozonated oils have demonstrated promising results for clinical applications. The reaction of ozone with the unsaturated compounds of oils produces by‐products such as ozonides and poly peroxides. A deeper knowledge of the dynamics of by‐product formation is helpful in determining the required ozonation degree to obtain therapeutic effects. The aim of this paper is to show the relationship between ozonation degree and structural and viscosity changes during the ozonation of grape seed (GS) and sunflower (SF) oils. Structural characterization was done by Fourier transform infrared (FT‐IR) and hydrogen‐1 nuclear magnetic resonance (1H NMR) spectroscopy, with iso‐ozonides being identified. Viscosity showed a significant increase during ozonation, a fact associated with poly peroxide formation. We have made use of the total unsaturation (TU) method to measure the ozonation degree. The TU of non‐ozonated GS oil was found to be higher than for SF oil (5.94 and 4.49 mmol per g of oil, respectively), and their by‐product distributions were also found to differ. In GS oil, three reaction steps were observed for double‐bond conversion into iso‐ozonides and poly peroxides, while the ozonides and poly peroxides were formed in parallel in SF oil. The studies we implemented characterized the differences in the reactivities of these oils with ozone. Practical applications: In this work, we propose using the TU method to measure the ozonation degree of ozonated oils. TU experimental determination is based on the ozonation of the sample, and the ozone‐oxidizable substrate is quantified. Despite GS and SF oils having similar fatty acid compositions, they contain other unsaturated compounds specific to their vegetal sources. These compounds are also reactive with ozone, and are also quantified by the TU method. The differences in distributions of by‐products among ozonated oils from different sources could explain why the ozonation degree need not be the same for different oils. Studies like this represent a feasibility foundation for controlling the therapeutic application of ozonated oils and correctly interpreting their well‐known clinical effects. Ozonated vegetable oils have many interesting applications in the food, cosmetic, and pharmaceutical industries, as well as in medicine. A deeper knowledge of the dynamics of by‐product formation is helpful in determining the required ozonation degree to obtain therapeutic effects. In this work, we propose using the total unsaturation method to measure the ozonation degree of ozonated oils. We showed the relationship between ozonation degree and structural and viscosity changes during the ozonation of grape seed and sunflower oils. The differences in distributions of by‐products among ozonated oils from different sources could explain why the ozonation degree need not be the same for different oils.

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Section: Ft-ir and 1 H Nmr Spectroscopic Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Correlation of structural characterization and viscosity measurements with total unsaturation: An effective method for controlling ozonation in the preparation of ozonated grape seed and sunflower oils

Guerra-Blanco¹,

Poznyak²,

Chairez³

et al. 2015

Euro J Lipid Sci & Tech

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“…The ozonide compositions have the capacity to deliver nascent oxygen deep within the lesion. Ozonated oil has been used topically for the treatment of different types of wounds, 12–14 but only a few studies reported the peroxide evaluation of the ozonated oil 15 . The present study was designed to evaluate the therapeutic effect of topical ozonated sesame oil (OSO) on acute cutaneous wound healing based on the level of peroxide species in a murine model.…”

mentioning

confidence: 99%

Ozonated sesame oil enhances cutaneous wound healing in SKH1 mice

Valacchi

Lim

Belmonte

et al. 2010

Wound Repair Regeneration

122

110

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Ozone is well recognized as a bactericidal agent and its beneficial effect on wound healing could be a consequence of this property. Because ozone itself does not penetrate the cells but immediately reacts with polyunsaturated fatty acids, its effects should be the results of oxidative reaction. For this reason, ozonated oils could be a way to deliver ozone messengers to the skin. This paper evaluated the therapeutic effects of three different grades of ozonated sesame oil in acute cutaneous wounds made in the skin of SKH1 mice. Specifically, wound closure rate, histological parameters, and the level of key proteins such as vascular endothelial growth factors and cyclin D1 have been analyzed in relation to the peroxide level present in the ozonated oil. Treatment with moderately ozonated sesame oil--expressed as peroxide value about 1,500)--has a faster wound closure rate in the first 7 days than treatment with oil containing either lower or higher peroxide value, and even with controls. Moreover, under the same treatment, an earlier and higher response of cells involved in wound repair, a higher angiogenesis, as well as an enhanced vascular endothelial growth factors and cyclin D1 expression were observed. The present study shows the validity of ozonated sesame oil in cutaneous wound healing and emphasizes the importance of the ozonation grade.

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“…O óleo ozonizado controla a cascata de eventos nas fases de reparação desencadeando e modulando a fase inflamatória possui efeitos pro-inflamatórios e antiinflamatórios, mas aplicações repetidas de óleo ozonizado na pele de ratos parecem produzir perda do pelo no local ou indução de reações de hipersensibilidade por contato, estes achados identificam uma nova função biológica do óleo ozonizado e indicam uma reação adversa nos casos de aplicações repetitivas (Kataoka et al, 2009;Kim et al, 2009). …”

Section: Medidas De Prevenção Do Quadro De Alveoliteunclassified

Estudo histomorfológico e histomorfométrico da reparação tecidual na alveolite provocada em ratos tratada por óleo ozonizado

Sánchez¹,

Pilar²

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A minha Orientadora Maria Cristina Zindel Deboni, a quem eu admiro muito, agradeço pela paciência e dedicação na orientação deste trabalho, obrigada por acreditar em mim. Sua orientação cientifica me proporcionaram um grande enriquecimento profissional e pessoal. Obrigada pelo apoio oportuno.A meus pais Elba e Antonio, exemplo de luta, disciplina, igualmente meus irmãos Beatriz, Elba e Paul, pela força e apoio que me oferecem cada dia para alcançar meus sonhos. Agradeço a Deus por ser abençoada com uma família maravilhosa.Agradeço aos professores, da Disciplina de Cirurgia da FOUSP, em especial a Profa. Dra. Maria da Graça Naclério Homem, pela oportunidade de realizar o curso de Pós-Graduação. Seu compromisso com a ciência eu levarei como lição.Às professoras: Dra. Luciana Corrêa, sempre atenciosa e disposta. Obrigada pelos ensinamentos e apoio quando solicitado. À Dra. Andrea Traina, pelas valiosa colaboração neste trabalho. Ao prof. Dr. Décio dos Santos Pinto Junior, por ter dedicado parte do seu tempo para realização das fotos.Às técnicas de laboratório e clínica: Cida e Natália, e aos secretários Rose, Ângela, Belira e Edson; por facilitar o desenvolvimento desta pesquisa.Aos técnicos do laboratório de patologia bucal Adriana, Elisa, Giovanni, Bia pela orientação na realização da parte laboratorial deste trabalho.As minhas novas amigas: Ana, Heliana, Jenny, Liz, Mario, Roberto e Sonia pela ajuda incondicional quando a seqüência de dificuldades na execução deste trabalho apareceu. Obrigada pela amizade.Aos meus colegas de turma: Alexandre, Gabriel, Leandro, Mariana, Ophir, Rodrigo e Samantha pelo coleguismo.

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Proinflammatory Event of Ozonized Olive Oil in Mice

Cited by 12 publications

References 15 publications

Correlation of structural characterization and viscosity measurements with total unsaturation: An effective method for controlling ozonation in the preparation of ozonated grape seed and sunflower oils

Correlation of structural characterization and viscosity measurements with total unsaturation: An effective method for controlling ozonation in the preparation of ozonated grape seed and sunflower oils

Ozonated sesame oil enhances cutaneous wound healing in SKH1 mice

Estudo histomorfológico e histomorfométrico da reparação tecidual na alveolite provocada em ratos tratada por óleo ozonizado

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