Project ILSAP: An inter-laboratory study on accuracy and precision in isotachophoresis

Trieling, RG; Kaniansky, Dušan

doi:10.1016/0021-9673(93)83428-u

Cited by 4 publications

(2 citation statements)

References 2 publications

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“…lience, ITP is a technique suitable for accurate and reproducible analyses of routine samples by using a slraightfcirward and well-defined procedure that is easily transferred from one laboratory to another. This was proved by the results of the ILSAP project [16] where each of 17 worldwide located laboratories analyzed lactate and creatinine in six model samples of aqueous solutions also containing 0.9% w/v NaCl. For concentration levels of 0.003 M, 0.01 M and 0.03 M, the reproducibility found was 0.00059 M, 0.00112 M and 0.00205 M for lactate and 0.00133 M, 0.00088 M and 0.00266 M for creatinine, respectively.…”

Section: Itp As An Analytical Techniquementioning

confidence: 84%

Recent application and developments of capillary isotachophoresis

Gebauer

Boček

1997

Electrophoresis

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Capillary isotachophoresis is a powerful electromigration separation method with a pronounced capability to concentrate trace components in diluted samples. At present, capillary isotachophoresis is utilized predominantly as the first step in on-line combination with capillary zone electrophoresis. This article is a continuation of previous reviews and summarizes the results published during 1993-1996.

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Section: Itp As An Analytical Techniquementioning

confidence: 84%

Recent application and developments of capillary isotachophoresis

Gebauer

Boček

1997

Electrophoresis

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“…Good results have been obtained by various research workers who analysed these substances by liquid chromatography [7][8][9][10][11][12][13][14], electrophoresis [15][16][17][18] and isotachophoresis [19][20][21][22][23][24][25][26]. Many references can easily be found and they are not cited here because only incomplete list could be given.…”

Section: Introductionmentioning

confidence: 99%

Separation of some hydroxycarboxylic acids by capillary isotachophoresis in the presence of neutral cyclodextrins

et al. 1999

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Complex formation in isotachophoresis is an important tool for improvement in the separation of sample ions. This possibility is mainly used for inorganic ions where the effective mobilities are less sensitive to pH changes. The possibility of influencing the effective mobilities of organic ions through complex formation may be useful in the separation of structurally related compounds with similar acid-base behaviour where the resolution cannot be achieved by varying the pH. The use of cyclodextrin as a neutral macrocyclic complex-forming agent seems to be advantageous for the resolution of similar organic compounds [1][2][3][4][5][6].The analysis of carboxylic acids, such as mono-, poly, and hydroxy-carboxylic acids, is extremely important and nearly all separation techniques have been applied to them. Good results have been obtained by various research workers who analysed these substances by liquid chromatography [7][8][9][10][11][12][13][14], electrophoresis [15][16][17][18] and isotachophoresis [19][20][21][22][23][24][25][26]. Many references can easily be found and they are not cited here because only incomplete list could be given. So far, little attention has been paid to the separation of hydroxycarboxylic acids by isotachophoresis.In this work, we discuss various electrolyte systems in which hydroxycarboxylic acids can be analysed by isotachophoresis. Several operational systems are considered below in order to show complex formation and the variations in the effective mobilities. Experimental ChemicalsDeionized and redistilled water was used in the preparation of the solutions of the electrolytes and compounds investigated. All chemicals were of the highest quality commercially available. The solutes investigated (Tab. I) were provided by Pharmaceutical Research Institute, Warsaw, Poland.Sample solutions (2 mmol/L) were prepared by dissolving each substance in water and were stored in a refrigerator. β-cyclodextrin (βCD) was obtained from Merck (Darmstadt). Heptakis (2,3,6-tri-O-methyl)-β-cyclodextrin (TMβCD) was synthesised by the method of Nowotny et al. [27]. ApparatusIsotachophoretic experiments were performed using a Villa Labeco ZKI 02 column-coupling isotachophoretic analyser (Slovak Republic) equipped with capillaries made of a fluorinated ethylene-propylene copolymer. Operating conditionsThe operating conditions are given in table II. In the case of one-dimensional isotachophoresis, the preseparation and the analytical capillary was filled with leading Abstract. The aim of this work was to study the various electrolyte systems in which hydroxycarboxylic acids can be separated by capillary isotachophoresis. Using β-cyclodextrin as additive to the leading electrolyte, the complete separation of hydroxycarboxylic acids was achieved. The results confirmed the significant influence of the cyclodextrin concentration and pH on the resolution of hydroxycarboxylic acids.

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