Prolactin in follicular fluid and intracellular store calcium in follicular cells are related to morphological signs of ovarian follicle atresia in cows: Work in progress

Lebedeva, I.; Denisenko, V. Yu.; Lebedev, V A; Kuzmina, T. I.

doi:10.1016/s0093-691x(98)00002-8

Cited by 19 publications

(8 citation statements)

References 28 publications

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“…This hormone participates in a variety of physiological processes important to reproduction (Freeman et al 2000). It is the main luteotropic hormone during gestation, supporting luteal progesterone secretion by decreasing the progesterone metabolising enzyme 20a-hydroxysteroid dehydrogenase (Lamprecht et al 1969;Bast and Melampy 1972;Morishige and Rothchild 1974), and is anti-atretic at the preovulatory follicle level by decreasing intracellular calcium concentrations (Lebedeva et al 1998). In African (Bechert et al 1999;Yamamoto et al 2010) but not Asian (Brown et al 2004a) elephants, prolactin increases in a cyclic pattern during the nonluteal phase of the oestrous cycle, and is presumed to be important for facilitating follicle selection and development similar to that described in other species (Yoshimura et al 1989(Yoshimura et al , 1991Larsen et al 1990;Li et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Evidence that hyperprolactinaemia is associated with ovarian acyclicity in female zoo African elephants

Dow

Brown

2012

Reprod. Fertil. Dev.

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African elephants of reproductive age in zoos are experiencing high rates of ovarian cycle problems (>40%) and low reproductive success. Previously, our laboratory found that 1/3 of acyclic females exhibit hyperprolactinaemia, a likely cause of ovarian dysfunction. This follow-up study re-examined hyperprolactinaemia in African elephants and found the problem has increased significantly to 71% of acyclic females. Circulating serum progestagens and prolactin were analysed in 31 normal cycling, 13 irregular cycling and 31 acyclic elephants for 12 months. In acyclic females, overall mean prolactin concentrations differed from cycling females (P < 0.05), with concentrations being either higher (n = 22; 54.90 ± 13.31 ngmL(-1)) or lower (n = 9; 6.47 ± 1.73 ngmL(-1)) than normal. No temporal patterns of prolactin secretion were evident in elephants that lacked progestagen cycles. In cycling females, prolactin was secreted in a cyclical manner, with higher concentrations observed during nonluteal (34.38 ± 1.77 and 32.75 ± 2.61 ngmL(-1)) than luteal (10.51 ± 0.30 and 9.67 ± 0.42 ngmL(-1)) phases for normal and irregular females, respectively. Of most concern was that over two-thirds of acyclic females now are hyperprolactinemic, a dramatic increase over that observed 7 years earlier. Furthermore, females of reproductive age constituted 45% of elephants with hyperprolactinaemia. Until the cause of this problem is identified and a treatment is developed, reproductive rates will remain suboptimal and the population nonsustaining.

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Section: Introductionmentioning

confidence: 99%

Evidence that hyperprolactinaemia is associated with ovarian acyclicity in female zoo African elephants

Dow

Brown

2012

Reprod. Fertil. Dev.

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“…This strategy exposes oocyte-cumulus complexes (OCCs) to a more 'physiological' -albeit undefinedhormonal and nutritional milieu. By contrast, undefined/-defined mammalian oocyte maturation systems -despite endocrine profiles conducive to cytoplasmic and nuclear maturation -contain a nutrient profile optimised for the culture of somatic cells rather than the combination of oocyte and cumulus cells (Zuelke & Brackett 1993, Carolan et al 1996, Eppig et al 1996, Lebedeva et al 1998, Smitz & Cortvrindt 1999, Watson et al 2000, Nagai 2001.…”

Section: Introductionmentioning

confidence: 99%

Fluctuations in bovine ovarian follicular fluid composition throughout the oestrous cycle

Orsi

Gopichandran²,

Leese³

et al. 2005

Reproduction

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Bovine oocyte maturation in vitro frequently results in abnormal cytoplasmic maturation and failure to acquire developmental competence. This is, in part, likely to be due to the non-physiological nutritional milieu to which oocytes are exposed. Improvements in oocyte developmental potential may be achieved by modelling nutrient profiles on those of preovulatory follicular fluid (FF). However, little is known about fluctuations in FF nutrient levels according to follicle dominance and oestrous cyclicity. This study therefore characterised the carbohydrate and amino acid profile of FF according to these parameters, and compared preovulatory FF composition with that of maturation medium. Carbohydrate concentrations (n 5 121) were determined enzymatically whilst amino acid profiles (n 5 40) were determined by reverse-phase HPLC. Pyruvate and glucose concentrations were unaffected by follicle dominance, whereas Stage III -IV lactate profiles were higher in non-dominant FF (P < 0.01). While most dominant FF amino acid concentrations were affected by oestrous stage, only glutamate, alanine, leucine and lysine levels fluctuated in non-dominant FF. Glucose and lactate concentrations were significantly negatively correlated, whereas most amino acids were significantly positively correlated with each other. Maturation medium had higher pyruvate and lower lactate concentrations than preovulatory FF (P < 0.001), whereas glucose level was similar. All amino acid levels (except histidine, taurine, alanine and tryptophan) differed significantly between maturation medium and preovulatory FF. These data indicated that FF composition varies throughout the oestrous cycle. Preovulatory FF nutrient profile differed from that of maturation medium, perhaps accounting for the poor developmental competence of in vitro matured oocytes. These data may contribute to the formulation of a nutritionally more physiological maturation medium.

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“…Lower concentration of PRL was associated with presence of pyknotic nuclei in granulosa cells which is an indicator of a late stage of cell death and follicular atresia [22, 67]. …”

Section: Discussionmentioning

confidence: 99%

Identification of potential biomarkers in donor cows for in vitro embryo production by granulosa cell transcriptomics

et al. 2017

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The Ovum Pick Up-In vitro Production (OPU-IVP) of embryos is an advanced reproductive technology used in cattle production but the complex biological mechanisms behind IVP outcomes are not fully understood. In this study we sequenced RNA of granulosa cells collected from Holstein cows at oocyte aspiration prior to IVP, to identify candidate genes and biological mechanisms for favourable IVP-related traits in donor cows where IVP was performed separately for each animal. We identified 56 genes significantly associated with IVP scores (BL rate, kinetic and morphology). Among these, BEX2, HEY2, RGN, TNFAIP6 and TXNDC11 were negatively associated while Mx1 and STC1 were positively associated with all IVP scores. Functional analysis highlighted a wide range of biological mechanisms including apoptosis, cell development and proliferation and four key upstream regulators (COX2, IL1, PRL, TRIM24) involved in these mechanisms. We found a range of evidence that good IVP outcome is positively correlated with early follicular atresia. Furthermore we showed that high genetic index bulls can be used in breeding without reducing the IVP performances. These findings can contribute to the development of biomarkers from follicular fluid content and to improving Genomic Selection (GS) methods that utilize functional information in cattle breeding, allowing a widespread large scale application of GS-IVP.

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Prolactin in follicular fluid and intracellular store calcium in follicular cells are related to morphological signs of ovarian follicle atresia in cows: Work in progress

Cited by 19 publications

References 28 publications

Evidence that hyperprolactinaemia is associated with ovarian acyclicity in female zoo African elephants

Evidence that hyperprolactinaemia is associated with ovarian acyclicity in female zoo African elephants

Fluctuations in bovine ovarian follicular fluid composition throughout the oestrous cycle

Identification of potential biomarkers in donor cows for in vitro embryo production by granulosa cell transcriptomics

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