Prolactin receptor, growth hormone receptor, and putative somatolactin receptor in Mozambique tilapia: Tissue specific expression and differential regulation by salinity and fasting

Pierce, Andrew L.; Fox, Bradley K.; Davis, Lori K.; Visitacion, N; Kitahashi, Takashi; Hirano, Tetsuya; Grau, E. Gordon

doi:10.1016/j.ygcen.2007.06.023

Cited by 120 publications

(83 citation statements)

References 48 publications

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“…We were unable to determine total muscle mRNA transcript levels as a function of body size during different metabolic states, as was done for the liver, because all muscle tissue cannot be reliably excised. Nevertheless, the elevations in skeletal muscle ghr2 mRNA during catabolism are consistent with that observed in gilthead sea bream, rainbow trout, and tilapia (Saera-Vila et al 2005, Pierce et al 2007, and suggest an alternative form of GH resistance. That is, signaling by GH through the Ghrs could be impaired (Beauloye et al 2002), leading to reduced Igf1 transcription as shown here.…”

Section: Discussionsupporting

confidence: 79%

“…3A), which is similar to that we previously reported with partial feed restriction in HSB . Studies by others using similar normalizations have reported either increases or no change in hepatic igf1 mRNA but declines in plasma Igf1 and/or growth following feed restriction (Ayson et al 2007, Pierce et al 2007. The lack of concordance in hepatic igf1 mRNA and circulating hormone in these studies may be resolved by the use of an alternative method of mRNA normalization presented here.…”

Section: Discussionmentioning

confidence: 63%

“…It should be noted that it is not certain whether the Ghr1 in non-salmonid fishes is a true Ghr (Jiao et al 2006, Li et al 2007 or instead the somatolactin receptor (SLR; Fukada et al 2005, Fukamachi et al 2005, Pierce et al 2007. Recent phylogentic and evolutionary analysis suggests that the SLR may in fact be a duplicated Ghr that occurred during the fish-specific genome duplication (Fukamachi & Meyer 2007).…”

Section: Jiaomentioning

confidence: 99%

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Regulation of endocrine and paracrine sources of Igfs and Gh receptor during compensatory growth in hybrid striped bass (Morone chrysops×Morone saxatilis)

Picha

Turano

Tipsmark

et al. 2008

Journal of Endocrinology

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Compensatory growth (CG) is a period of growth acceleration that exceeds normal rates after animals are alleviated of certain growth-stunting conditions. In hybrid striped bass (HSB, Morone chrysops!Morone saxatilis), 3 weeks of complete feed restriction results in a catabolic state that, when relieved, renders a subsequent phase of CG. The catabolic state was characterized by depressed levels of hepatic Type I and II GH receptor (ghr1, ghr2) and igf1 mRNA, along with considerable decreases in plasma Igf1. The state of catabolism also resulted in significant declines in hepatic igf2 mRNA and in circulating 40 kDa Igfbinding protein (Igfbp). Skeletal muscle expression of ghr2 mRNA was significantly increased. Upon realimentation, specific growth rates (SGRs) were significantly higher than sized-matched controls, indicating a period of CG. Hepatic ghr1, ghr2, igf1 and igf2 mRNA levels along with plasma Igf1 and 40 kDa Igfbp increased rapidly during realimentation. Plasma Igf1 and total hepatic igf2 mRNAwere significantly correlated to SGR throughout the study. Skeletal muscle igf1 mRNA also increased tenfold during CG. These data suggest that endocrine and paracrine/autocrine components of the GH-Igf axis, namely igf1, igf2, and ghr1 and ghr2, may be involved in CG responses in HSB, with several of the gene expression variables exceeding normal levels during CG. We also demonstrate that normalization of hepatic mRNA as a function of total liver production, rather than as a fraction of total RNA, may be a more biologically appropriate method of quantifying hepatic gene expression when using real-time PCR.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 63%

Section: Jiaomentioning

confidence: 99%

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Regulation of endocrine and paracrine sources of Igfs and Gh receptor during compensatory growth in hybrid striped bass (Morone chrysops×Morone saxatilis)

Picha

Turano

Tipsmark

et al. 2008

Journal of Endocrinology

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“…We also explored the effects of acclimation salinity and extracellular osmolality on the mRNA expression of two Prl receptors (prlr) in the RPD to explore possible feedback mechanisms on Prl expression/ release. Originally cloned by Sandra et al (1995) in Nile tilapia (Oreochromis niloticus), the tilapia Prl receptor (PrlR1) is expressed in an array of tissues, including gill, intestine and kidney (Pierce et al 2007). prlr1 mRNA expression is sensitive to salinity, with enhanced expression occurring in the gill when Mozambique tilapia are transferred from SW to FW (Breves et al 2011) and down-regulated expression after transfer from FW to SW (Breves et al 2010a).…”

Section: Journal Of Endocrinology (2012) 213 89-98mentioning

confidence: 99%

“…All primer pairs are previously described: prlr1 (Pierce et al 2007), prlr2 (long isoform; Breves et al 2010b), ostf1 (Breves et al 2010a), prl 177 and prl 188 (Magdeldin et al 2007) and ef1-a (Breves et al 2010b). The quantitative real-time PCRs were set up as previously described (Pierce et al 2007). Briefly, 200 nM of each primer, 3 ml cDNA and 12 ml SYBR Green PCR Master Mix (Applied Biosystems) were added to 15 ml final reaction volume.…”

Section: Quantitative Real-time Pcrmentioning

confidence: 99%

Prolactin177, prolactin188 and prolactin receptor 2 in the pituitary of the euryhaline tilapia, Oreochromis mossambicus, are differentially osmosensitive

Seale

Moorman²,

Stagg³

et al. 2012

Journal of Endocrinology

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Two forms of prolactin (Prl), prolactin 177 (Prl 177 ) and prolactin 188 (Prl 188 ), are produced in the rostral pars distalis (RPD) of the pituitary gland of euryhaline Mozambique tilapia, Oreochromis mossambicus. Consistent with their roles in fresh water (FW) osmoregulation, release of both Prls is rapidly stimulated by hyposmotic stimuli, both in vivo and in vitro. We examined the concurrent dynamics of Prl 177 and Prl 188 hormone release and mRNA expression from Prl cells in response to changes in environmental salinity in vivo and to changes in extracellular osmolality in vitro. In addition, mRNA levels of Prl receptors 1 and 2 (prlr1 and prlr2) and osmotic stress transcription factor 1 (ostf1) were measured. Following transfer from seawater (SW) to FW, plasma osmolality decreased, while plasma levels of Prl 177 and Prl 188 and RPD mRNA levels of prl 177 and prl 188 increased. The opposite pattern was observed when fish were transferred from FW to SW. Moreover, hyposmotically induced release of Prl 188 was greater in Prl cells isolated from FW-acclimated fish after 6 h of incubation, while the hyposmotically induced increase in prl 188 mRNA levels was only observed in SW-acclimated fish. In addition, prlr2 and ostf1 mRNA levels in Prl cells from both FW-and SW-acclimated fish increased in direct proportion to increases in extracellular osmolality both in vivo and in vitro. Taken together, these results indicate that the osmosensitivity of the tilapia RPD is modulated by environmental salinity with respect to hormone release and gene expression. Journal of Endocrinology (2012) 213, 89-98Introduction A necessary component of the systems that direct osmoregulatory processes are osmoreceptors that detect deviations from salt and water balance and initiate adaptive responses to restore equilibrium. The prolactin (Prl)-secreting cells of the euryhaline Mozambique tilapia (Oreochromis mossambicus) comprise a nearly homogeneous portion of the rostral pars distalis (RPD) of the pituitary (conservatively O95%) and are directly stimulated by physiologically relevant decreases in extracellular osmolality in vitro , Seale et al. 2006b. Consistent with its role as an essential hormone for fresh water (FW) acclimation (reviewed by Hirano (1986), Manzon (2002) and Sakamoto & McCormick (2006)) plasma Prl levels increase in vivo when tilapia are transferred from seawater (SW) to FW (Yada et al. 1994, Seale et al. 2002. The operation of tilapia Prl cells as true osmoreceptors and the anatomical organization of these cells in tilapia RPD provide an accessible model to integrate osmosensing mechanisms with gene transcription, hormone synthesis and hormone secretion (Seale et al. 2005).Hyposmotically induced Prl release from tilapia lactotrophs is dependent on the entry of extracellular Ca 2C through stretch-activated ion channels upon cell swelling (Seale et al. 2003). There is evidence that the influx of extracellular Ca 2C may have distinct intracellular effects depending on the acclimation salinity of the animal fro...

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Gene expression of growth hormone family and glucocorticoid receptors, osmosensors, and ion transporters in the gill during seawater acclimation of Mozambique tilapia, Oreochromis mossambicus

et al. 2010

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This study characterized endocrine and ionoregulatory responses accompanying seawater (SW) acclimation in Mozambique tilapia (Oreochromis mossambicus). Changes in plasma hormones and gene expression of hormone receptors, putative osmosensors, and ion transporters in the gill were measured. Transfer of freshwater (FW)-acclimated tilapia to SW resulted in a marked elevation in plasma osmolality and a significant rise in plasma growth hormone (GH) levels at 12 hr and 14 days after transfer. Significant reductions in plasma prolactin (PRL(177) and PRL(188)) levels also occurred in SW-transferred fish; no effect of transfer upon plasma cortisol or insulin-like growth factor I was observed. Gene expression of GH receptor increased strongly 6 hr after transfer, whereas PRL receptor was lower than controls at 12 hr. By contrast, mRNA levels of somatolactin and glucocorticoid receptors were unaffected by SW transfer. Osmotic stress transcription factor 1 mRNA levels rose significantly between 3 and 12 hr, whereas the calcium-sensing receptor was unaffected. Aquaporin-3 gene expression was strongly down-regulated during SW acclimation from 12 hr until the conclusion of the experiment. Na(+)/K(+)/2Cl(-) cotransporter gene expression increased significantly 3 hr after transfer, whereas expression of Na(+)/Cl(-) cotransporter, specific to FW-type chloride cells, declined by 6 hr into SW acclimation. The response of Na(+)/H(+) exchanger was less pronounced, but showed a similar pattern to that of the Na(+)/Cl(-) cotransporter. These results suggest that acquisition of hyposmoregulatory mechanisms in Mozambique tilapia entails the coordinated interaction of systemic hormones with local factors in the gill, including hormone receptors, ion transporters, and osmosensors.

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Prolactin receptor, growth hormone receptor, and putative somatolactin receptor in Mozambique tilapia: Tissue specific expression and differential regulation by salinity and fasting

Cited by 120 publications

References 48 publications

Regulation of endocrine and paracrine sources of Igfs and Gh receptor during compensatory growth in hybrid striped bass (Morone chrysops×Morone saxatilis)

Regulation of endocrine and paracrine sources of Igfs and Gh receptor during compensatory growth in hybrid striped bass (Morone chrysops×Morone saxatilis)

Prolactin177, prolactin188 and prolactin receptor 2 in the pituitary of the euryhaline tilapia, Oreochromis mossambicus, are differentially osmosensitive

Gene expression of growth hormone family and glucocorticoid receptors, osmosensors, and ion transporters in the gill during seawater acclimation of Mozambique tilapia, Oreochromis mossambicus

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