Prolidase

Abstract

Prolidase (EC.3.4.13.9) or proline dipeptidase, is one of the unique enzyme capable of degrading dipeptides, in which a proline or hydroxyproline residue is located at the C-terminal position. Prolidase has a unique function in all cell types; therefore, the mechanisms and parameters involved in prolidase activity regulation are of special interest. Could prolidase be a good biomarker in different physiologic and pathologic conditions? This is an important question. There is no consensus on the answer to this question. It is of great importance during collagen turnover, infl ammation, tissue fi brosis and skeletal abnormalities. Prolidase itself without other biochemical markers may not provide information to clinicians about disease activity. So, I think it should be evaluated together with other serum biochemical markers. This review will serve to discuss many in vivo functions of prolidase, as well as level prolidase activity in diagnosis and monitoring of treatment in the various diseases (Ref. 50). Text in PDF www.elis.sk.

Functional Screening of Candidate Causal Genes for Insulin Resistance in Human Preadipocytes and Adipocytes

“…PEPD, a type a prolidase, is involved in the formation and maintenance of extracellular matrix by recycling proline for synthesis of collagen and other proline-containing proteins. In general, prolidase directly binds and activates epidermal growth factor receptor and stimulates cell growth and proliferation 39 . Serum prolidase activity was associated with the presence and severity of coronary artery disease 40 .…”

Rectal application of argan oil improves healing of colorectal anastomosis in rats

“…Prolidase plays a significant role in splitting proline from imidodipeptides for synthesis of collagen and other prolinecontaining proteins. Prolidase deficiency is an autosomal recessive disorder characterized by severe imidodipeptiduria and increased plasma levels of proline-containing dipeptides with various symptoms, such as mental retardation, recurrent infections, skin lesions, and collagen tissue abnormalities 21,22 . In the current study, the prolidase levels were statistically significantly higher in the control group than in the argan oil and sham groups (p<0.05 for both groups).…”

High-level expression and molecular characterization of a recombinant prolidase fromEscherichia coliNovaBlue

“…Although the exact physiological role of prokaryotic prolidases remains to be elucidated, eukaryotic enzymes are thought to be involved in the recycling of collagen-derived proline ( Hu, Phang & Valle, 2008 ). In this regard, activity fluctuations in eukaryotic-type prolidase can serve as an indicator of dysfunctional collagen metabolism as well as disease progression ( Kitchener & Grunden, 2012 ; Namiduru, 2016 ).…”