Proliferation activity in oral and cutaneous canine melanocytic tumours: correlation with histological parameters, location, and clinical behaviour

Millanta, Francesca; Fratini, Filippo; Corazza, Michele; Castagnaro, Massimo; Zappulli, Valentina; Poli, Alessandro

doi:10.1016/s0034-5288(02)00041-3

Cited by 43 publications

(95 citation statements)

References 11 publications

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“…Those cells, whose nuclei presented a brown color, regardless of intensity, were considered positive for immunohistochemistry with anti-MIB-1, while negative cells presented basophilic nucleus. In this study, Ki-67 expression was higher in the malignant trichoepithelioma, when compared to the other hair follicle tumors analyzed, similar to what was observed in mastocytomas of grade III (Seguin et al, 2006), lymphosarcomas (Scase et al, 2006), malignant melanomas (Millanta et al, 2002), spinocellular carcinomas (Della Salda et al, 2002), and malignant tumors of mammary gland (Lohr et al, 1997).…”

Section: Discussionsupporting

confidence: 84%

“…NOR and Ki-67 did not have significant statistic correlation (r=-0.5506). No significant correlation was observed between NOR and Ki-67 in neoplasms of mammary (Ceccarelli et al, 2000), lung (Soomro and Whimster et al, 1990), intestines (Nanashima et al, 1999), and female genital tract (Chung et al, 1994). This may be due to the fact that these methods quantified different phases of cell proliferative activity (Ceccarelli et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

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Association of nucleolar organizing regions and Ki-67 expression with recurrence rate of hair follicle tumor in dogs

Souza

Ocarino²,

Tavares³

et al. 2008

Arq. Bras. Med. Vet. Zootec.

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Mitotic index, nuclear diameter, number of nucleolar organizing regions, and Ki-67 expression, in hair follicle tumors of 82 dogs were evaluated. Tissue specimens were used to prepare sections for histological staining for number of nucleolar organizing region and immunohistochemical staining for Ki-67. Tumors were classified as trichoblastoma (n=32), benign trichoepithelioma (n=30), pilomatricoma (n=7), malignant trichoepithelioma (n=6), infundibular keratinizing acanthoma (n=5), and tricholemmoma (n=2). Head, dorsum, and limbs were the most affected sites. Malignant trichoepithelioma presented significantly higher mitotic index, number of nucleolar organizing regions and Ki-67 expression. Regarding benign neoplasms, trichoblastoma presented significantly higher mitotic index and number of nucleolar organizing regions. Ki-67 expression did not differ among hair follicle benign neoplasms. Recurrence was only observed in two cases, with one benign trichoepithelioma and one malignant trichoepithelioma. In the two cases, nodules have not been removed with clean surgical margin. It was concluded that in benign neoplasms of hair follicles, the number of nucleolar organizing regions and Ki-67 expression were significantly smaller than in malignant neoplasm. Clean surgical margins are suggested to be more responsible to tumor recurrences than the number of nucleolar organizing regions, expression of Ki-67, and the mitotic index.Keywords: dog, neoplasm, AgNOR, hair follicle, Ki-67 RESUMO O objetivo deste trabalho foi avaliar o índice mitótico, o diâmetro nuclear, o número de regiões organizadoras de nucléolos e a expressão do Ki-67 em 82 tumores de folículo piloso de cães, entre 2000 e 2006. Os tumores foram classificados como tricoblastoma (n=32), tricoepitelioma benigno (n=30), tricoepitelioma maligno (n=6), pilomatricoma (n=7), acantoma infundibular ceratinizante (n=5) e tricolemoma (n=2). A cabeça, o dorso e os membros foram os locais mais frequentemente acometidos. O tricoepitelioma maligno apresentou índice mitótico, número de regiões organizadoras de nucléolos e expressão do

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Association of nucleolar organizing regions and Ki-67 expression with recurrence rate of hair follicle tumor in dogs

Souza

Ocarino²,

Tavares³

et al. 2008

Arq. Bras. Med. Vet. Zootec.

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“…RACK1 overexpression was visible in every lesion over the whole specimen. Using the mitotic index as a parameter with prognostic significance for canine melanocytic neoplasms, 3,15,20,25 we were able to confirm the diagnosis of our sample collection. We showed here that the cellular distribution of RACK1 labeling distinguishes malignant from benign forms.…”

Section: Discussionmentioning

confidence: 60%

“…Epidemiological analysis of our samples indicated that our cohort was representative of reported dog melanoma. 8,12,20,21,31 Immunostaining, ApoTome Microscopy Analysis of RACK1 Distribution, and Ki-67 Index…”

Section: Dog Tissuesmentioning

confidence: 99%

Canine Melanoma Diagnosis

et al. 2013

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Melanoma diagnosis in dogs can be challenging due to the variety of histological appearances of canine melanocytic neoplasms. Markers of malignancy are needed. Receptor for activated C-kinase 1 (RACK1) was found to characterize melanomas in other mammals. We investigated the value of RACK1 detection in the classification of 19 cutaneous and 5 mucosal melanocytic neoplasms in dogs. These tumors were categorized as melanocytomas or benign and melanomas or malignant after evaluation of their morphology, mitotic index, and Ki-67 growth fraction. Using immunofluorescence, we confirmed microphthalmia-associated transcription factor (MITF) as a marker of normal and transformed melanocytic cells in dog tissues. All control (n ¼ 10) and tumoral (n ¼ 24) samples stained positively for MITF (34/34, 100%). Whereas RACK1 was not detected in healthy skin melanocytes, melanocytic lesions were all positive for RACK1 signal (24/24, 100%). RACK1 cytoplasmic staining appeared with 2 distinct distribution patterns: strong, diffuse, and homogeneous or granular and heterogeneous. All melanoma samples (13/13, 100%) stained homogeneously for RACK1. All melanocytomas (11/11, 100%) stained heterogeneously for RACK1. Immunohistochemistry was less consistent than immunofluorescence for all labelings in melanocytic lesions, which were often very pigmented. Thus, the fluorescent RACK1-MITF labeling pattern helped to distinguish melanomas from melanocytomas. Furthermore, RACK1 labeling correlated with 2 of 11 morphological features linked to malignancy: cell and nuclear size. These results suggest that RACK1 may be used as a marker in dog melanomas.

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“…A idade média dos cães acometidos foi de 10,7 anos. O melanoma é mais comum em cães velhos com idade média de 9,8 anos (Millanta et al 2002) a 11,6 anos (Bolon et al 1990). Cães com a pele pigmentada são mais predispostos a apresentarem neoplasias melanocíticas (Ramos-Vara et al 2000).…”

Section: Discussionunclassified

Melanoma amelanótico em cães: estudo retrospectivo de 35 casos (2004-2010) e caracterização imuno-histoquímica

et al. 2012

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Pesq. Vet. Bras. 32(4):340-346, abril 2012 340 RESUMO.-Melanomas são tumores agressivos de melanócitos que ocorrem principalmente na cavidade oral, nas junções mucocutâneas e na pele de cães. Este tipo de neoplasma pode apresentar diversos graus de pigmentação melânica, incluindo total ausência (melanomas amelanóticos [MA] Melanomas are aggressive tumors of melanocytes. They are common in dogs and involve mainly the oral cavity, mucocutaneous junction, and skin. Furthermore, these tumors could be highly pigmented or lack pigment. The biopsies archives from SPV-UFRGS, 2004 to 2010, were retrieved and melanocytic neoplasms in dogs were revised. A retrospective study of 35 cases of amelanotic melanomas (AM) was performed, also immunohistochemistry (IHC) characterization was evaluated. The dogs more affected were mixed breed followed by Poodle, Dachshund and Cocker Spaniel. The average age of the dogs was 10.7 years (5-18 years in age) and there was no sex predilection. The locations of the neoplasms were the oral cavity (57.1%) and digits (17.1%). Histologically, 40% were classiϐied as epithelioid, 34.3% mixed and 25.7% spindle. The positive immunostaining for vimentin, S-100 protein and melan-A were 86.6%, 70%, and 56.6% respectively. These results indicated the most affected dogs with AM were elderly. Epiteliod classiϐication was the most observed histologically. It is important to perform IHC, due to lacking of differentiation of AM, mainly, anti S-100 protein that showed to be the best option of positive marker, even better to Melan-A. racterização pela imuno-histoquímica (IHQ). As principais raças acometidas foram o Poodle, Dachshund e Cocker Spaniel, mas o maior número de casos foi observado em cães sem raça deϐinida (SRD). A idade média desses cães foi de 10,7 anos (variação de 5 a 18 anos) e não houve predileção por sexo. As principais localizações incluíram cavidade oral (57,1%) e dígitos (17,1%). Histologicamente, 40% dos MA foram classiϐicados como epitelioides, 34,3% como mistos e 25,7% como fusiformes. Na avaliação IHQ, 86,6% dos casos foram positivos para a vimentina, 70% para a proteína S-100 e 56,6% para o melan-A. Os resultados obtidos neste trabalho possibilitam concluir que os cães com MA caracterizavam-se por serem velhos. A forma celular mais observada foi a epitelioide. Devido a pouca diferenciação desses tumores, ressalta-se a importância da realização do painel imuno-histoquímico, sobretudo da proteína S-100, que apresentou melhor marcação que o melan-A.

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Proliferation activity in oral and cutaneous canine melanocytic tumours: correlation with histological parameters, location, and clinical behaviour

Cited by 43 publications

References 11 publications

Association of nucleolar organizing regions and Ki-67 expression with recurrence rate of hair follicle tumor in dogs

Association of nucleolar organizing regions and Ki-67 expression with recurrence rate of hair follicle tumor in dogs

Canine Melanoma Diagnosis

Melanoma amelanótico em cães: estudo retrospectivo de 35 casos (2004-2010) e caracterização imuno-histoquímica

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