Proliferation and differentiation of osteoblastic cells on silicon-doped TiO2 film deposited by cathodic arc

Wang, Bing; Sun, Junying; Shi, Qian; Liu, Xuanyong; Zhang, Shailin; Liu, Fei; Dong, Shengjie; Zha, Guo-Chun

doi:10.1016/j.biopha.2012.08.008

Cited by 14 publications

(10 citation statements)

References 36 publications

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“…Therefore, although there was no marked difference between the cell proliferation on Si–TiO 2 -NT and TiO 2 -NT substrates, the osteogenic activity of cells was markedly improved when they were cultured on Si–TiO 2 -NT compared to both TiO 2 -NT and Ti substrates. The fact that Si doping affected the differentiation of cells, but not their proliferation, on TiO 2 -NT treated surfaces slightly differs from our previous finding, in which Si-doped TiO 2 films appeared to favor both osteoblastic proliferation and differentiation [ 32 ]. This might be the result of the topographical difference (rough TiO 2 nanotubes versus relatively smooth TiO 2 film surface).…”

Section: Discussioncontrasting

confidence: 97%

“…As expected, MC3T3-E1 cells showed more spread morphology and extended more filopodia when cultured on both Si–TiO 2 -NT and TiO 2 -NT substrates compared to those on Ti surfaces ( Figure 3 and Figure 4 ). This is in accordance with a number of previous studies in which both surface micro-/nano-topography and Si doping were found to markedly affect cellular behaviors [ 32 , 39 , 40 ]. All substrates did not show any cytotoxicity.…”

Section: Discussionsupporting

confidence: 93%

“…Recently, we have found that, compared to pure TiO 2 , Si-doped TiO 2 coatings prepared using cathodic arc deposition [ 32 , 33 ] or micro-arc oxidation [ 24 ] favored the activities of osteoblastic cells. In this study, we were interested in examining whether Si doping could further enhance the osteogenic potential of TiO 2 nanotubes.…”

Section: Introductionmentioning

confidence: 99%

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Silicon-Doped Titanium Dioxide Nanotubes Promoted Bone Formation on Titanium Implants

Zhao

Wang

Shi

et al. 2016

IJMS

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While titanium (Ti) implants have been extensively used in orthopaedic and dental applications, the intrinsic bioinertness of untreated Ti surface usually results in insufficient osseointegration irrespective of the excellent biocompatibility and mechanical properties of it. In this study, we prepared surface modified Ti substrates in which silicon (Si) was doped into the titanium dioxide (TiO2) nanotubes on Ti surface using plasma immersion ion implantation (PIII) technology. Compared to TiO2 nanotubes and Ti alone, Si-doped TiO2 nanotubes significantly enhanced the expression of genes related to osteogenic differentiation, including Col-I, ALP, Runx2, OCN, and OPN, in mouse pre-osteoblastic MC3T3-E1 cells and deposition of mineral matrix. In vivo, the pull-out mechanical tests after two weeks of implantation in rat femur showed that Si-doped TiO2 nanotubes improved implant fixation strength by 18% and 54% compared to TiO2-NT and Ti implants, respectively. Together, findings from this study indicate that Si-doped TiO2 nanotubes promoted the osteogenic differentiation of osteoblastic cells and improved bone-Ti integration. Therefore, they may have considerable potential for the bioactive surface modification of Ti implants.

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Section: Discussioncontrasting

confidence: 97%

Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Silicon-Doped Titanium Dioxide Nanotubes Promoted Bone Formation on Titanium Implants

Zhao

Wang

Shi

et al. 2016

IJMS

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“…Some nonadherent cells were not removed and exhibited small and round shape. Previous research also showed that cellular activity and growth were influenced by the surface characteristics such as topography . Another phenomenon was found that cells on the margin of collagen hydrogel were very crowded, seen as Figure (D2), which demonstrated that the collagen hydrogel contracted from circumference to the center.…”

Section: Resultsmentioning

confidence: 99%

Biological behavior of fibroblast on contractile collagen hydrogel crosslinked by γ‐irradiation

Zhang

Chen

et al. 2013

J Biomedical Materials Res

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Collagen hydrogels exhibited a contractile trend in simulated body fluid. In this study, the internal pore architecture and mechanical properties of collagen hydrogel prepared by radiation crosslinking was evaluated during contraction, and the effect of contractile collagen hydrogels on the biological behavior of fibroblasts were investigated in vitro, such as viability, proliferation, morphology, apoptosis, cycle, and stress fiber. The results showed that accompany with contraction of collagen hydrogel, the pore diameter of the hydrogels decreased and compressive modulus increased. However, fibroblasts can grow on contractile collagen hydrogels. Indeed, collagen hydrogel contracted from circumference to the interior, which retard the spreading of fibroblasts on the dynamic substrate and interrupted the initial attachment of the cell. However, contraction of collagen hydrogel had not only significant influence on the L929 cell proliferation, but also accelerated the apoptosis. Cell cycle analysis showed that contractile collagen hydrogel may promote cell cycle from G0/G1 phase to S phase, and DNA synthesis and cell proliferation were enhanced, but which may be different in contraction process. Therefore, as a scaffold for tissue engineering, the strategy for inhibition of the contraction of collagen hydrogel should be taken into account.

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“…TiO 2 nanotubes with varying dimensions have been shown to regulate MSC differentiation . Silicon‐doped TiO 2 coatings (Si‐TiO 2 ) formed by either micro or cathodic arc oxidation, have also recently been shown to improve the response of osteogenic cells, although it is important to point out that the Si‐TiO 2 films were porous and therefore not capable of fully blocking out the underlying bulk properties of the CoCrMo. Nevertheless, the enhanced osteogenic response from Si‐TiO 2 presents a possible next‐step for potentially increasing the biocompatibility of CoCrMo further, via the inclusion of Si into the TiO 2 CVD coatings.…”

Section: Discussionmentioning

confidence: 99%

TiO₂‐coated CoCrMo: Improving the osteogenic differentiation and adhesion of mesenchymal stem cells in vitro

Logan

Sherif

Cross

et al. 2014

J Biomedical Materials Res

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The current gold standard material for orthopedic applications is titanium (Ti), however, other materials such as cobalt-chromium-molybdenum (CoCrMo) are often preferred due to their wear resistance and mechanical strength. This study investigates if the bioactivity of CoCrMo can be enhanced by coating the surface with titanium oxide (TiO 2 ) by atmospheric pressure chemical vapor deposition (CVD), thereby replicating the surface oxide layer found on Ti. CoCrMo, TiO 2 -coated CoCrMo (CCMT) and Ti substrates were used for this study. Cellular f-actin distribution was shown to be noticeably different between cells on CCMT and CoCrMo after 24 h in osteogenic culture, with cells on CCMT exhibiting greater spread with developed protrusions. Osteogenic differentiation was shown to be enhanced on CCMT compared to CoCrMo, with increased calcium ion content per cell (p < 0.05), greater hydroxyapatite nodule formation (p < 0.05) and reduced type I collagen deposition per cell (p < 0.05). The expression of the focal adhesion protein vinculin was shown to be marginally greater on CCMT compared to CoCrMo, whereas AFM results indicated that CCMT required more force to remove a single cell from the substrate surface compared to CoCrMo (p < 0.0001). These data suggest that CVD TiO 2 coatings may have the potential to increase the biocompatibility of CoCrMo implantable devices.

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Proliferation and differentiation of osteoblastic cells on silicon-doped TiO2 film deposited by cathodic arc

Cited by 14 publications

References 36 publications

Silicon-Doped Titanium Dioxide Nanotubes Promoted Bone Formation on Titanium Implants

Silicon-Doped Titanium Dioxide Nanotubes Promoted Bone Formation on Titanium Implants

Biological behavior of fibroblast on contractile collagen hydrogel crosslinked by γ‐irradiation

TiO₂‐coated CoCrMo: Improving the osteogenic differentiation and adhesion of mesenchymal stem cells in vitro

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Proliferation and differentiation of osteoblastic cells on silicon-doped TiO2 film deposited by cathodic arc

Cited by 14 publications

References 36 publications

Silicon-Doped Titanium Dioxide Nanotubes Promoted Bone Formation on Titanium Implants

Silicon-Doped Titanium Dioxide Nanotubes Promoted Bone Formation on Titanium Implants

Biological behavior of fibroblast on contractile collagen hydrogel crosslinked by γ‐irradiation

TiO2‐coated CoCrMo: Improving the osteogenic differentiation and adhesion of mesenchymal stem cells in vitro

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Product

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TiO₂‐coated CoCrMo: Improving the osteogenic differentiation and adhesion of mesenchymal stem cells in vitro