Proliferation and Osteogenic Differentiation of Mesenchymal Stem Cells Cultured onto Three Different Polymers In Vitro

Jäger, Markus; Feser, T.; Denck, H.; Krauspe, Rüdiger

doi:10.1007/s10439-005-5889-2

Cited by 73 publications

(53 citation statements)

References 75 publications

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“…26,27 All volunteer donors had given written consent according to the Declaration of Helsinki in its present form.…”

Section: Cell Culturementioning

confidence: 99%

“…26 Alkaline Phosphatase (ALP; Blue Alkaline Phosphatase Substrate Kit III, #SK-5300, Vector Laboratories, Burlingame, CA), Receptor-activator of NFkBligand [RANKL; rabbit-anti, 1:100/PBSTA (10 mL/mL), Santa Cruz SC-9073, Santa Cruz Biotechnology, Santa Cruz, CA], Twist (rabbit-anti, 1:200/PBSTA, Santa Cruz SC-15393, Santa Cruz Biotechnology, Santa Cruz, CA). Signal was enhancement by an anti-rabbit-IgG/biotin secondary antibody system (anti-rabbit-IgG/biotinylated, avidin-biotin-complex, Vector; þDAB, Sigma).…”

Section: Immuncytochemical Stainingsmentioning

confidence: 99%

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Dexamethasone modulates BMP‐2 effects on mesenchymal stem cells in vitro

Jäger

Fischer

Dohrn

et al. 2008

Journal Orthopaedic Research

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Dexamethasone/ascorbic acid/glycerolphosphate (DAG) and bone morphogenic protein (BMP)-2 are potent agents in cell proliferation and differentiation pathways. This study investigates the in vitro interactions between dexamethasone and BMP-2 for an osteoblastic differentiation of mesenchymal stem cells (MSCs). Bone marrow-derived human MSCs were cultured with DAG (group A), BMP-2 þ DAG (group B), and DAG þ BMP-2 combined with a porous collagen I/III scaffold (group C). RT-PCR, ELISA, immuncytochemical stainings and flow cytometry analysis served to evaluate the osteogenic-promoting potency of each of the above conditions in terms of cell morphology/viability, antigen presentation, and gene expression. DAG induced collagen I secretion from MSCs, which was further increased by the combination of DAG þ BMP-2. In comparison, the collagen scaffold and the control samples showed no significant influence on collagen I secretion of MSCs. DAG stimulation of MSCs led also to a steady but not significant increase of BMP-2 level. A DAG and more, a DAG þ BMP-2, stimulation increased the number of mesenchymal cells (CD105þ/CD73þ). All samples showed mRNA of ALP, osteopontin, Runx2, Twist 1 and 2, Notch-1/2, osteonectin, osteocalcin, BSP, and collagen-A1 after 28 days of in vitro culture. Culture media of all samples showed a decrease in Ca 2þ and PO 4 2À concentration, whereas a collagen-I-peak only occurred at day 28 in DAG-and DAG þ BMP-2-stimulated bone marrow cells. In conclusion, BMP-2 enhances DAG-induced osteogenic differentiation in mesenchymal bone marrow cells. Both agents interact in various ways and can modify osteoblastic bone formation. ß

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“…26,27 All volunteer donors had given written consent according to the Declaration of Helsinki in its present form.…”

Section: Cell Culturementioning

confidence: 99%

Section: Immuncytochemical Stainingsmentioning

confidence: 99%

Dexamethasone modulates BMP‐2 effects on mesenchymal stem cells in vitro

Jäger

Fischer

Dohrn

et al. 2008

Journal Orthopaedic Research

View full text Add to dashboard Cite

show abstract

“…(14) In addition, USSCs were also cultivated onto ␤-TCP granules [␤-Ca 3 (PO 4 ) 2 , Ca/P ‫ס‬ 1.50, 90% interconnected pore space, particle size: 100 nm, pore size: 1-1000 m; Vitoss, Fa. Orthovita], which had shown good cytocompatibility properties in vitro.…”

Section: Biomaterialsmentioning

confidence: 99%

Bone Healing and Migration of Cord Blood—Derived Stem Cells Into a Critical Size Femoral Defect After Xenotransplantation

Jäger

Degistirici

Knipper

et al. 2007

Journal of Bone and Mineral Research

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Stem cell and tissue engineering-based therapies have become a promising option to heal bony defects in the future. Human cord blood-derived mesenchymal stem cells were seeded onto a collagen/ tricalcium phosphate scaffold and xenotransplanted into critical size femoral defects of 46 nude rats. We found a survival of human cells within the scaffold and surrounding bone/bone marrow up to 4 wk after transplantation and an increased bone healing rate compared with controls without stem cells. This study supports the application of cord blood stem cells for bone regeneration. Introduction:The treatment of critical size bone defects is still a challenging problem in orthopedics. In this study, the survival, migration, and bone healing promoting potency of cord blood-derived stem cells were elucidated after xenotransplantation into a critical size femoral defect in athymic nude rats. Materials and Methods: Unrestricted somatic stem cells (USSCs) isolated from human cord blood were tested toward their mesenchymal in vitro potency and cultivated onto a collagen I/III and ␤-tricalcium phosphate (␤-TCP) scaffold. The biomaterial-USSC composite was transplanted into a 4-mm femoral defect of 40 nude rats and stabilized by an external fixator. Twelve animals without USSCs served as controls. Cell survival, migration, and bone formation were evaluated by blood samples, X-rays, and histological and immunocytochemical analysis of different organs within a maximal postoperative follow-up of 10 wk. Results: Of the 52 nude rats, 46 animals were evaluated (drop-out rate: 11.5%). Human-derived stem cells showed an engraftment within the scaffold and adjacent femur up to 4 wk after xenotransplantation. With further time, the human cells were destroyed by the host organism. We found a significant increase in bone formation in the study group compared with controls. USSC transplantation did not significantly influence blood count or body weight in athymic nude rats. Whereas the collagen I/III scaffold was almost resorbed 10 wk after transplantation, there were still significant amounts of TCP present in transplantation sites at this time. Conclusions:Human cord blood-derived stem cells showed significant engraftment in bone marrow, survived within a collagen-TCP scaffold up to 4 wk, and increased local bone formation in a nude rat's femoral defect.

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“…In particular, these cells are of high interest for bone tissue engineering, since autologous MSCs can be easily isolated from a patient´s bone marrow, show a high proliferation potential and can be ex vivo differentiated toward the osteoblastic lineage without difficulty (Marion & Mao, 2006). As already demonstrated elsewhere (Jager et al, 2005;Rodriguez et al, 2004), osteogenic 13 differentiation of expanded MSCs was inducible by simply changing the growth medium to differentiation medium (containing dexamethasone, β-glycerolphosphate and ascorbic acid).…”

Section: Discussionmentioning

confidence: 90%