Proliferation and osteogenic differentiation of human periodontal ligament cells on akermanite and β-TCP bioceramics

Xia, Lunguo; Zhang, Zhiyuan; Chen, Lei; Zhang, Wenjie; Zeng, Deliang; Zhang, Xiuli; Chang, Jiang; Jiang, Xinquan

doi:10.22203/ecm.v022a06

Cited by 100 publications

(73 citation statements)

References 59 publications

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“…For cell morphology, 30 cells were seeded on the substrates in a 24-well plate at a density of 5.0×10 4 cells per mL. The nonadhered cells were removed by washing three times with phosphate-buffered saline after 24 hours of culture.…”

Section: Cell Morphology and Proliferationmentioning

confidence: 99%

Magnesium ion implantation on a micro/nanostructured titanium surface promotes its bioactivity and osteogenic differentiation function

Wang¹,

Li²,

Zhang³

et al. 2014

IJN

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As one of the important ions associated with bone osseointegration, magnesium was incorporated into a micro/nanostructured titanium surface using a magnesium plasma immersion ion-implantation method. Hierarchical hybrid micro/nanostructured titanium surfaces followed by magnesium ion implantation for 30 minutes (Mg30) and hierarchical hybrid micro/nanostructured titanium surfaces followed by magnesium ion implantation for 60 minutes (Mg60) were used as test groups. The surface morphology, chemical properties, and amount of magnesium ions released were evaluated by field-emission scanning electron microscopy, energy dispersive X-ray spectroscopy, field-emission transmission electron microscopy, and inductively coupled plasma-optical emission spectrometry. Rat bone marrow mesenchymal stem cells (rBMMSCs) were used to evaluate cell responses, including proliferation, spreading, and osteogenic differentiation on the surface of the material or in their medium extraction. Greater increases in the spreading and proliferation ability of rBMMSCs were observed on the surfaces of magnesium-implanted micro/nanostructures compared with the control plates. Furthermore, the osteocalcin ( OCN ), osteopontin ( OPN ), and alkaline phosphatase ( ALP ) genes were upregulated on both surfaces and in their medium extractions. The enhanced cell responses were correlated with increasing concentrations of magnesium ions, indicating that the osteoblastic differentiation of rBMMSCs was stimulated through the magnesium ion function. The magnesium ion-implanted micro/nanostructured titanium surfaces could enhance the proliferation, spreading, and osteogenic differentiation activity of rBMMSCs, suggesting they have potential application in improving bone-titanium integration.

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Section: Cell Morphology and Proliferationmentioning

confidence: 99%

Magnesium ion implantation on a micro/nanostructured titanium surface promotes its bioactivity and osteogenic differentiation function

Wang¹,

Li²,

Zhang³

et al. 2014

IJN

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“…Interestingly, both N2 and N5 composites showed an early onset of Ca release (Figures 3 and 4). The release of Ca 2+ ions from HA 38 and other Ca-containing materials, such as beta-tricalcium phosphate 38,39 or akermanite, 39,40 has been shown to increase osteogenic cell differentiation, including the expression and synthesis of osteopontin. The addition of Ca 2+ ions directly to the culture media also stimulated the production of osteopontin.…”

mentioning

confidence: 99%

Support for the initial attachment, growth and differentiation of MG-63 cells: a comparison between nano-size hydroxyapatite and micro-size hydroxyapatite in composites

Filová¹,

Suchý²,

Sucharda³

et al. 2014

IJN

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Hydroxyapatite (HA) is considered to be a bioactive material that favorably influences the adhesion, growth, and osteogenic differentiation of osteoblasts. To optimize the cell response on the hydroxyapatite composite, it is desirable to assess the optimum concentration and also the optimum particle size. The aim of our study was to prepare composite materials made of polydimethylsiloxane, polyamide, and nano-sized (N) or micro-sized (M) HA, with an HA content of 0%, 2%, 5%, 10%, 15%, 20%, 25% (v/v) (referred to as N0–N25 or M0–M25), and to evaluate them in vitro in cultures with human osteoblast-like MG-63 cells. For clinical applications, fast osseointegration of the implant into the bone is essential. We observed the greatest initial cell adhesion on composites M10 and N5. Nano-sized HA supported cell growth, especially during the first 3 days of culture. On composites with micro-size HA (2%–15%), MG-63 cells reached the highest densities on day 7. Samples M20 and M25, however, were toxic for MG-63 cells, although these composites supported the production of osteocalcin in these cells. On N2, a higher concentration of osteopontin was found in MG-63 cells. For biomedical applications, the concentration range of 5%–15% (v/v) nano-size or micro-size HA seems to be optimum.

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“…Recently, many investigators have demonstrated that implanted -TCP is resorbed by macrophagelike cells without cytotoxicity or inflammatory response [33,34]. -TCP degradation promotes concentration of calcium ions released into the regenerative space and alkaline phosphatase activity of osteoblastic cells [35,36]; these phenomena allow environmental changes for osteogenesis to occur. Histological specimens treated with the Journal of Nanomaterials nano-TCP scaffold at 10 days revealed foreign-body giant cells around the relatively large particles of -TCP in the scaffold.…”

Section: Discussionmentioning

confidence: 99%

Osteoconductivity and Biodegradability of Collagen Scaffold Coated with Nano‐β‐TCP and Fibroblast Growth Factor 2

Ibara

Miyaji

Fugetsu

et al. 2013

Journal of Nanomaterials

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Nanoparticle bioceramics have become anticipated for biomedical applications. Highly bioactive and biodegradable scaffolds would be developed using nanoparticles of -tricalcium phosphate ( -TCP). We prepared collagen scaffolds coated by nano--TCP and fibroblast growth factor 2 (FGF2) and evaluated the effects on new bone augmentation and biodegradation. The collagen sponge was coated with the nano-TCP dispersion and freeze-dried. Scaffold was characterized by SEM, TEM, XRD, compressive testing and cell seeding. Subsequently, the nano--TCP/collagen scaffold, collagen sponge, and each material loaded with FGF2 were implanted on rat cranial bone. As a control, no implantation was performed. Nano-TCP particles were found to be attached to the fibers of the collagen sponge by SEM and TEM observations. Scaffold coated with nano-TCP showed higher compressive strength and cytocompatibility. In histological evaluations at 10 days, inflammatory cells were rarely seen around the residual scaffold, suggesting that the nano-TCP material possesses good tissue compatibility. At 35 days, bone augmentation and scaffold degradation in histological samples receiving nano--TCP scaffold were significantly greater than those in the control. By loading of FGF2, advanced bone formation is facilitated, indicating that a combination with FGF2 would be effective for bone tissue engineering.

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Proliferation and osteogenic differentiation of human periodontal ligament cells on akermanite and β-TCP bioceramics

Cited by 100 publications

References 59 publications

Magnesium ion implantation on a micro/nanostructured titanium surface promotes its bioactivity and osteogenic differentiation function

Magnesium ion implantation on a micro/nanostructured titanium surface promotes its bioactivity and osteogenic differentiation function

Support for the initial attachment, growth and differentiation of MG-63 cells: a comparison between nano-size hydroxyapatite and micro-size hydroxyapatite in composites

Osteoconductivity and Biodegradability of Collagen Scaffold Coated with Nano‐β‐TCP and Fibroblast Growth Factor 2

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