Proliferation and triglyceride synthesizing activities of fibroblast-like cells derived from epididymal and subcutaneous adipose tissues of rats

Inadera, Hidekuni; Ishikawa, Yoh; Shirai, Kohji; Saitō, Yasushi; Yoshida, Sho

doi:10.1080/00365519309088413

Cited by 3 publications

(1 citation statement)

References 12 publications

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“…The relationship between subcutaneous and visceral adipocyte metabolism and development has been extensively studied using primary cell cultures isolated from fat tissue [20]. We have shown that adipocytes which accumulated in mesenteric area, and not in the subcutaneous area, induce TNF-a gene expression, thus leading to an increased concentration of TNF-a in the serum using a cell transplantation method in mice [14].…”

Section: Discussionmentioning

confidence: 99%

Activation of diacylglycerol O‐acyltransferase 1 gene results in increased tumor necrosis factor‐α gene expression in 3T3‐L1 adipocytes

Hirata¹,

Unoki²,

Bujo³

et al. 2006

FEBS Letters

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The tumor necrosis factor-a (TNF-a) expression has been reported to be largely dependent on the size of adipocytes. We herein investigated the gene regulation of diacylglycerol Oacyltransferase (DGAT) in order to clarify the mechanism of TNF-a expression induced in large adipocytes. 3T3-L1 cells were cultured in the presence of 5 mM or 25 mM glucose to generate adipocytes from which the triglyceride content differs. The expression of TNF-a, DGAT1, and DGAT2 were upregulated in adipocytes cultured with 25 mM glucose. Furthermore, knockdown of DGAT1 gene significantly inhibited the TNF-a expression. Finally, the DGAT1 expression levels were closely related to the TNF-a level in 3T3-L1 adipocytes.

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Section: Discussionmentioning

confidence: 99%

Activation of diacylglycerol O‐acyltransferase 1 gene results in increased tumor necrosis factor‐α gene expression in 3T3‐L1 adipocytes

Hirata¹,

Unoki²,

Bujo³

et al. 2006

FEBS Letters

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Molecular analysis of lipid‐depleting factor in a colon‐26‐inoculated cancer cachexia model

Inadera¹,

Nagai²,

Dai³

et al. 2002

Intl Journal of Cancer

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Cachexia in cancer is characterized by progressive emaciation involving depletion of host adipose tissue stores, the molecular mechanism of which remains largely unknown. In this study, we have attempted to clarify the biologic characteristics of lipid-depleting factor in a mouse cachexia model. Utilizing differentiated 3T3-L1 adipocytes, we established an assay method quantifying the lipid-depleting activity in plasma derived from colon-26-inoculated mice and then analyzed the associated molecular mechanism. Injection (s.

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Different Effect of Growth Hormone on the Triglyceride Metabolism in Visceral and Subcutaneous AdiposeTissues of Rats

Shirai¹,

Itoh

Miyashita

et al. 1996

Clin Pediatr Endocrinol

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To clarify the mechanism by which visceral adipose tissue developed in growth hormone (GH) deficient patients, and was reduced by GH supplementation , the effects of GH on the triglyceride metabolism in the visceral and subcutaneous adipose tissues of the rats were compared. Visceral and subcutaneous adipose tissues were obtained from omentum and abdominal wall. [14C]deoxyglucose uptake and the triglyceride synthetic activity from [14C]oleic acid , and heparin-releasable lipoprotein lipase activity were not changed by the addition of GH (10 ng/mL) in both visceral and subcutaneous adipose tissues , but the lipolysis by GH (10 ng/mL)was much more remarkable in the visceral adipose tissue than in subcutaneous adipose tissue , whereas adrenaline-induced lipolysis occurred almost equally in both tissues. These results suggest that the preponderance of visceral adipose tissue in GH deficiency might partly be due to the lack of GH-preferential lipolysis in visceral adipose tissue.

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Proliferation and triglyceride synthesizing activities of fibroblast-like cells derived from epididymal and subcutaneous adipose tissues of rats

Cited by 3 publications

References 12 publications

Activation of diacylglycerol O‐acyltransferase 1 gene results in increased tumor necrosis factor‐α gene expression in 3T3‐L1 adipocytes

Activation of diacylglycerol O‐acyltransferase 1 gene results in increased tumor necrosis factor‐α gene expression in 3T3‐L1 adipocytes

Molecular analysis of lipid‐depleting factor in a colon‐26‐inoculated cancer cachexia model

Different Effect of Growth Hormone on the Triglyceride Metabolism in Visceral and Subcutaneous AdiposeTissues of Rats

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