Proliferation and viability of adherent cells manipulated by standing-wave ultrasound in a microfluidic chip

Hultström, Jessica; Manneberg, Otto; Dopf, Katja; Hertz, Hans M.; Brismar, Hjalmar; Wiklund, Martin

doi:10.1016/j.ultrasmedbio.2006.07.024

Cited by 206 publications

(176 citation statements)

References 26 publications

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“…(27) gives an expression for ∅ ;using this and the relation ∅ = × ∅ , Eqns. (19) or (20) will give ∅ . This allows a description of the pressure field in the air gap, bearing in mind that is given by Eqn.…”

Section: Modelling Of Air-filled Resonators a Details Of The Modelsmentioning

confidence: 99%

“…These non-linear forces act directly on the suspended matter, causing a migration over multiple oscillation cycles [18]. Acoustic manipulation, using ARF, has been widely applied in microfluidic devices, due to good biocompatibility [19], relatively simple instrumentation, robust architectures and good on-chip integration possibilities. Capabilities such as positioning of particles in a single plane for filtration (acoustic filters) [20,21], within a microfluidic channel [22] and within three dimensions [23] have been demonstrated.…”

Section: Introductionmentioning

confidence: 99%

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Optimisation of an acoustic resonator for particle manipulation in air

Devendran

Billson

Hutchins

et al. 2016

Sensors and Actuators B: Chemical

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(2015) Optimisation of an acoustic resonator for particle manipulation in air. Sensors and Actuators B: Chemical, 224 . pp. 529-538. Permanent WRAP URL:http://wrap.warwick.ac.uk/83726 Copyright and reuse:The Warwick Research Archive Portal (WRAP) makes this work by researchers of the University of Warwick available open access under the following conditions. Copyright © and all moral rights to the version of the paper presented here belong to the individual author(s) and/or other copyright owners. To the extent reasonable and practicable the material made available in WRAP has been checked for eligibility before being made available.Copies of full items can be used for personal research or study, educational, or not-for-profit purposes without prior permission or charge. Provided that the authors, title and full bibliographic details are credited, a hyperlink and/or URL is given for the original metadata page and the content is not changed in any way. AbstractAn acoustic resonator system has been investigated for the manipulation and entrapment of micronsized particles in air. Careful consideration of the effect of the thickness and properties of the materials used in the design of the resonator was needed to ensure an optimised resonator. This was achieved using both analytical and finite-element modelling, as well as predictions of acoustic attenuation in air as a function of frequency over the 0.8 to 2.0 MHz frequency range. This resulted in a prediction of the likely operational frequency range to obtain particle manipulation. Experimental results are presented to demonstrate good capture of particles as small as 15 µm in diameter.

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Section: Modelling Of Air-filled Resonators a Details Of The Modelsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Optimisation of an acoustic resonator for particle manipulation in air

Devendran

Billson

Hutchins

et al. 2016

Sensors and Actuators B: Chemical

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“…(2)], which is dependent on the density and compressibility of the particles compared to the surrounding medium. Studies have shown that ultrasonic cell manipulation in microfluidic systems are gentle to cells (16,17). Dense particles (i.e., cells) tend to have a positive contrast factor in most commonly used flow media including water, and they, consequently, focus into the acoustic pressure nodes.…”

Section: Microfluidic Systemmentioning

confidence: 99%

Label‐free somatic cell cytometry in raw milk using acoustophoresis

Grenvall

Folkenberg²,

Augustsson

et al. 2012

Cytometry Pt A

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A microfluidic system for cell enumeration in raw milk was developed. The new method, preconditions the milk sample using acoustophoresis that removes lipid particles which are larger than a few micrometers. The acoustophoretic preprocessing eliminates the need for conventional sample preparation techniques, which include chemical solvents, cell labeling and centrifugation, and facilitates rapid cell enumeration using microscopy or coulter counter measurements. By introducing an acoustic standing wave with three pressure nodes in a microchannel at the same time as the milk sample is laminated to the channel center, lipids are acoustically driven to the closest pressure antinode at each side of the channel center and the cells in the milk sample are focused in the central pressure node. The extracted center fraction with cells becomes sufficiently clean from lipid vesicles to enable enumeration of somatic cells without any labeling step either by direct light microscopy or by coulter counting. Obtained lipid free milk fractions clearly revealed the cell fraction when analyzed by Coulter Counting.

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“…Such systems are particularly promising for the handling of micron-scale biological cells for applications such as preparation [2,3] or fractionation [4,5] of samples prior to analysis, the formation of aggregates for cell interaction studies [6], or for biosensor enhancement [7,8]. Although high-intensity ultrasound can be used to destroy cells [9], there is now a significant body of evidence to show that, at the energy levels required for particle manipulation, cell viability is maintained [10,11] along with the propensity of the cells to proliferate following exposure [12].…”

Section: Introductionmentioning

confidence: 99%

Mode-switching: A new technique for electronically varying the agglomeration position in an acoustic particle manipulator

et al. 2010

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Acoustic radiation forces offer a means of manipulating particles within a fluid. Much interest in recent years has focussed on the use of radiation forces in microfluidic (or "lab on a chip") devices. Such devices are well matched to the use of ultrasonic standing waves in which the resonant dimensions of the chamber are smaller than the ultrasonic wavelength in use. However, such devices have typically been limited to moving particles to one or two predetermined planes, whose positions are determined by acoustic pressure nodes/antinodes set up in the ultrasonic standing wave. In most cases devices have been designed to move particles to either the centre or (more recently) the side of a flow channel using ultrasonic frequencies that produce a half or quarter wavelength over the channel respectively.It is demonstrated here that by rapidly switching back and forth between half and quarter wavelength frequencies -mode-switching -a new agglomeration position is established that permits beads to be brought to any arbitrary point between the half and quarter wave nodes. This new agglomeration position is effectively a position of stable equilibrium. This has many potential applications, particularly in cell sorting and manipulation. It should also enable precise control of agglomeration position to be maintained regardless of manufacturing tolerances, temperature variations, fluid medium characteristics and particle concentration.

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Proliferation and viability of adherent cells manipulated by standing-wave ultrasound in a microfluidic chip

Cited by 206 publications

References 26 publications

Optimisation of an acoustic resonator for particle manipulation in air

Optimisation of an acoustic resonator for particle manipulation in air

Label‐free somatic cell cytometry in raw milk using acoustophoresis

Mode-switching: A new technique for electronically varying the agglomeration position in an acoustic particle manipulator

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