Proliferation arrest, selectivity, and chemosensitivity enhancement of cancer cells treated by a low-intensity alternating electric field

Lei, Kin Fong; Hsieh, Shao-Chieh; Goh, Andrew; Kuo, Rei-Lin; Tsang, Ngan‐Ming

doi:10.1007/s10544-018-0339-8

Cited by 14 publications

(19 citation statements)

References 26 publications

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“…The protocol followed the description in the literature. 30,31 Thus, the HeLa cells expressed green fluorescence, and the HEL299 cells showed red fluorescence in the following experiments.…”

Section: Methodsmentioning

confidence: 83%

“…To easily observe the cells under a microscope, HeLa and HEL299 cells were, respectively, transduced with open reading frames (ORFs) of enhanced green fluorescent protein (EGFP) and red fluorescent protein (RFP). The protocol followed the description in the literature. , Thus, the HeLa cells expressed green fluorescence, and the HEL299 cells showed red fluorescence in the following experiments.…”

Section: Methodsmentioning

confidence: 99%

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Visualization and Quantification of 3D Tumor Cell Migration under Extracellular Stimulation

Goh

Yeh

Lei

2020

ACS Appl. Bio Mater.

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To investigate tumor cell migration capability, the scratch/wound healing assay and the Transwell assay are the most commonly used assays in the current biomedical research laboratory. However, both assays have their limitations and may mislead the interpretation of the results. In the current study, visualization and quantification of tumor cell migration process was realized in a three-dimensional (3D) environment. The tumor cells horizontally migrated along a Matrigel-filled microchannel under extracellular stimulation. The cell migration process was visualized under a microscope, and the migration speed could be calculated based on the traveling distance of the cells and the time required. Here, three demonstrations were conducted, respectively, including cells attracted by nutrient gradient, stimulated by cytokine, and coculturing with fibroblasts. The results revealed that the cell migration capability could be visually and quantitatively correlated to the extracellular stimulation. The current protocol is compatible to the existing laboratory setup and provides a persuasive result for the study of the 3D cell migration process. Understanding of the molecular and intercellular mechanism of cancer metastasis can potentially develop effective therapeutic strategy.

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Section: Methodsmentioning

confidence: 83%

Section: Methodsmentioning

confidence: 99%

Visualization and Quantification of 3D Tumor Cell Migration under Extracellular Stimulation

Goh

Yeh

Lei

2020

ACS Appl. Bio Mater.

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“…Significantly, no new safety signals were observed in this post-marketing safety surveillance analysis, which is an important observation considering that a child’s brain is still in development. Although in vivo and in vitro data have shown that TTFields selectively targets cancer cells, with minimal impact on healthy cells, additional data showing the effects of TTFields on myelinating cells would further support the safety of TTFields therapy in pediatric patients ( 40 – 42 ).…”

Section: Discussionmentioning

confidence: 99%

Safety of Tumor Treating Fields (TTFields) therapy in pediatric patients with malignant brain tumors: Post-marketing surveillance data

et al. 2022

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There is an unmet need to develop effective and tolerable treatments for pediatric patients with malignant central nervous system tumors. This is especially essential for pediatric patients with aggressive brain tumors such as high-grade gliomas which have a typical survival rate of under 2 years. Tumor Treating Fields (TTFields) are locoregional, noninvasive electric fields that produce an antimitotic effect on cancerous cells when applied to the skin via arrays. TTFields therapy (200 kHz) is currently approved in adult patients with newly diagnosed glioblastoma (GBM), with temozolomide, and recurrent GBM as monotherapy. Positive preclinical and clinical data have encouraged off-label use of TTFields therapy in pediatric patients with brain tumors, and this study aims to explore the safety of TTFields therapy in pediatric patients (0–18 years of age) based on data from an unsolicited post-marketing surveillance safety database. The real-world data reported here demonstrate that TTFields therapy has a favorable safety profile for pediatric patients with brain tumors, with no new safety signals observed. Findings from this study warrant further research into the efficacy of TTFields therapy, as well as its potential impact on the quality of life in pediatric patients.

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“…The transduction protocol followed the steps reported in the literature studies. 30,31 The transduced Huh7, HeLa, and Hel299 cells were selected with puromycin and then maintained in DMEM with 10% FBS.…”

Section: Cells and Transduction Of Fluorescent Proteinsmentioning

confidence: 99%

Cell marathon: long-distance cell migration and metastasis-associated gene analysis using a folding paper system

Huang

Lei

2022

Lab Chip

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Proliferation arrest, selectivity, and chemosensitivity enhancement of cancer cells treated by a low-intensity alternating electric field

Cited by 14 publications

References 26 publications

Visualization and Quantification of 3D Tumor Cell Migration under Extracellular Stimulation

Visualization and Quantification of 3D Tumor Cell Migration under Extracellular Stimulation

Safety of Tumor Treating Fields (TTFields) therapy in pediatric patients with malignant brain tumors: Post-marketing surveillance data

Cell marathon: long-distance cell migration and metastasis-associated gene analysis using a folding paper system

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