2004
DOI: 10.1002/jcb.20016
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Proliferation marker pKi‐67 occurs in different isoforms with various cellular effects

Abstract: The Ki-67 antigen, pKi-67, is a commonly used proliferation marker in research and pathology. It has been recognized that the protein exists in two different splice variants that differ in one exon. In the current work, we present three new splice variants of human pKi-67 consisting of two naturally occurring isoforms and one atypical version. Additionally, data is presented indicating that alternative splicing of the pKi-67 N-terminus is common in tumor cell lines. Analyzing 93 tissues mainly consisting of br… Show more

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Cited by 24 publications
(34 citation statements)
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“…The abovementioned clones detect different epitopes on the Ki-67 protein: BGX-Ki-67 (a fragment close to C-terminus) and MIB-1 ("Ki-67 motif" in exon 13). Hence, the difference in staining patterns obtained with MIB-1 and BGX-Ki-67 clones (cytoplasmic/membranous vs nuclear) might be the result of different Ki-67 splicing [22][23][24] or the presence of its mutations changing the structure of Ki-67. 25 Hypothetical "breakage" of Ki-67 protein might influence its cellular localization.…”
Section: Discussionmentioning
confidence: 99%
“…The abovementioned clones detect different epitopes on the Ki-67 protein: BGX-Ki-67 (a fragment close to C-terminus) and MIB-1 ("Ki-67 motif" in exon 13). Hence, the difference in staining patterns obtained with MIB-1 and BGX-Ki-67 clones (cytoplasmic/membranous vs nuclear) might be the result of different Ki-67 splicing [22][23][24] or the presence of its mutations changing the structure of Ki-67. 25 Hypothetical "breakage" of Ki-67 protein might influence its cellular localization.…”
Section: Discussionmentioning
confidence: 99%
“…The fact that they are both significantly correlated to metastasis suggest that they both code functional proteins. It is known that MKI67 is found in multiple isoforms that effect cellular processes differently [33]. …”
Section: Discussionmentioning
confidence: 99%
“…Alternative splicing of the mRNA of pKi-67 results in a short version without exon 7 . Analyzing 93 tissues mainly consisting of brain tumor specimens we recently found evidence that long and short isoform can be expressed independently of each other (Schmidt et al 2004). Moreover, we presented evidence that the pKi-67 N-terminus is multiply spliced resulting in at least five different isoforms which fulfill different functions in cell cycle control.…”
Section: Introductionmentioning
confidence: 97%