Proliferation of human primary vascular smooth muscle cells depends on serum response factor

Werth, Daniela; Grassi, Gabriele; Konjer, Nina; Dapas, Barbara; Farra, Rossella; Giansante, Carlo; Kandolf, Reinhard; Guarnieri, Gianfranco; Nordheim, Alfred; Heidenreich, Olaf

doi:10.1016/j.ejcb.2009.12.002

Cited by 42 publications

(36 citation statements)

References 49 publications

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“…This concept is also supported by the earlier transgenic study showing that complete knockout of SRF was lethal (Arsenian et al, 1998). Similar consequences have been also observed in transgenics of skeletal muscle (Li et al, 2005;Chavret et al, 2006;Lahoute et al, 2008) and smooth muscle (Miano et al, 2004;Werth et al, 2010). SRE has also been identified in cytoskeletal genes (ACTB, CFL1, DES, DSTN, TTN, KRT17, etc.…”

Section: Functions Of Srfsupporting

confidence: 66%

Gastric Ulcer Healing – Role of Serum Response Factor

Chai¹

2011

Peptic Ulcer Disease

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Section: Functions Of Srfsupporting

confidence: 66%

Gastric Ulcer Healing – Role of Serum Response Factor

Chai¹

2011

Peptic Ulcer Disease

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“…27 Recently, siRNA-mediated knockdown of SRF in human coronary artery SMCs resulted in decreases in both SMC migration and proliferation. 57 Thus, the decrease in SMC investment of the embryonic aorta where SRF has been deleted could be a consequence of reduced growth, migration, or a combination of both processes.…”

Section: Blood Vesselsmentioning

confidence: 99%

Role of serum response factor in the pathogenesis of disease

Miano

2010

Laboratory Investigation

123

100

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Serum response factor (SRF) is a ubiquitously expressed transcription factor that binds to a DNA cis element known as the CArG box, which is found in the proximal regulatory regions of over 200 experimentally validated target genes. Genetic deletion of SRF is incompatible with life in a variety of animals from different phyla. In mice, loss of SRF throughout the early embryo results in gastrulation defects precluding analyses in individual organ systems. Genetic inactivation studies using conditional or inducible promoters directing the expression of the bacteriophage Cre recombinase have shown a vital role for SRF in such cellular processes as contractility, cell migration, synaptic activity, inflammation, and cell survival. A growing number of experimental and human diseases are associated with changes in SRF expression, suggesting that SRF has a role in the pathogenesis of disease. This review summarizes data from experimental model systems and human pathology where SRF expression is either deliberately or naturally altered. Genomic DNA is a quaternary code comprising proteincoding and non-protein-coding sequences. While the protein-coding sequences are well-defined and increasingly understood, we are only beginning to elucidate the hidden information within the vast landscape of the non-proteincoding sequences. The field of comparative genomics has facilitated the identification of transcription factor-binding sites (TFBS) and microRNAs (miRs), which, aside from repetitive DNA sequences, are among the more easily decipherable non-protein-coding sequences in the human genome. Together, TFBS and miRs have essential roles in cell fate determination and cellular homeostasis of most life forms. Sequence variations (eg, single-nucleotide polymorphisms, SNPs) in the TFBS, miRs, and miR target sequences alter gene/protein expression and thus disturb homeostasis leading to disease. 1-4 A major imperative, therefore, is decoding the non-protein-coding genome relating to gene regulation to gain a full understanding of how DNA-binding transcription factors and the estimated one million or more TFBS direct normal biological processes.Serum response factor (SRF) is the founding member of the MADS-box family of transcription factors 5 and is one of the best understood DNA-binding proteins in the human proteome. The DNA-binding properties of SRF and its molecular cloning were first defined in the laboratory of Richard Treisman. 6,7 SRF has relatively low intrinsic transcriptional activity, but its interaction with over 60 cofactors confers strong transactivation potential in a cell-and context-specific manner. At least two major signaling pathways converge upon SRF to direct the programs of gene expression. 8,9 The classic pathway involves growth factor stimulation and mitogen-activated protein kinase signaling leading to the phosphorylation of the SRF cofactor, ELK1, and the activation of growth-related genes. 10,11 The second regulatory pathway occurs through Rho-dependent changes in actin dynamics. 12 In this pathway, si...

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“…Cell cycle phase evaluation and BrdU incorporation were performed as described, 32 and cells were analyzed by flow cytometry (FACSCanto, BD Biosciences, San Jose, CA, USA; DIVA software, Dialogic, Quebec, MTL, Canada). ImageJ software was used to measure the length of individual neurites in ten images for each experiment at a magnification of ×100; for this analysis, the cells were stained with Dil.…”

Section: Analysis Of Cell Proliferation and Differentiationmentioning

confidence: 99%

A nanoporous surface is essential for glomerular podocyte differentiation in three-dimensional culture

Zennaro¹,

Rastaldi²,

Bakeine³

et al. 2016

IJN

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Although it is well recognized that cell–matrix interactions are based on both molecular and geometrical characteristics, the relationship between specific cell types and the three-dimensional morphology of the surface to which they are attached is poorly understood. This is particularly true for glomerular podocytes – the gatekeepers of glomerular filtration – which completely enwrap the glomerular basement membrane with their primary and secondary ramifications. Nanotechnologies produce biocompatible materials which offer the possibility to build substrates which differ only by topology in order to mimic the spatial organization of diverse basement membranes. With this in mind, we produced and utilized rough and porous surfaces obtained from silicon to analyze the behavior of two diverse ramified cells: glomerular podocytes and a neuronal cell line used as a control. Proper differentiation and development of ramifications of both cell types was largely influenced by topographical characteristics. Confirming previous data, the neuronal cell line acquired features of maturation on rough nanosurfaces. In contrast, podocytes developed and matured preferentially on nanoporous surfaces provided with grooves, as shown by the organization of the actin cytoskeleton stress fibers and the proper development of vinculin-positive focal adhesions. On the basis of these findings, we suggest that in vitro studies regarding podocyte attachment to the glomerular basement membrane should take into account the geometrical properties of the surface on which the tests are conducted because physiological cellular activity depends on the three-dimensional microenvironment.

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Proliferation of human primary vascular smooth muscle cells depends on serum response factor

Cited by 42 publications

References 49 publications

Gastric Ulcer Healing – Role of Serum Response Factor

Gastric Ulcer Healing – Role of Serum Response Factor

Role of serum response factor in the pathogenesis of disease

A nanoporous surface is essential for glomerular podocyte differentiation in three-dimensional culture

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