Proliferation of rat small hepatocytes after long-term cryopreservation

Ikeda, Sei; Mitaka, Toshihiro; Harada, Keisuke; Sugimoto, Shinichi; Hirata, Kohichi; Mochizuki, Yohichi

doi:10.1016/s0168-8278(02)00069-7

Cited by 35 publications

(29 citation statements)

References 32 publications

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“…As previously reported (Mitaka et al, 1999;Ikeda et al, 2002), colonies consisting of 30 to 50 cells were observed at day 14 after plating. To collect SHs, the colonies were detached from dishes and immediately replated or cryopreserved at Ϫ80°C until use (Fig.…”

Section: Methodssupporting

confidence: 71%

Cytochrome P450 Expression of Cultured Rat Small Hepatocytes after Long-Term Cryopreservation

Ooe

Kon²,

Miyamoto³

et al. 2006

Drug Metab Dispos

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ABSTRACT:Small hepatocytes (SHs) are hepatic progenitor cells that can be cryopreserved for a long time. After thawing, the cells can proliferate and, when treated with Matrigel, they can differentiate into mature hepatocytes (MHs). In this study, we investigated whether cryopreserved SHs could express cytochromes P450 (P450s), whether P450 expression was induced by appropriate inducers, and whether P450 activities were measurable.

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Section: Methodssupporting

confidence: 71%

Cytochrome P450 Expression of Cultured Rat Small Hepatocytes after Long-Term Cryopreservation

Ooe

Kon²,

Miyamoto³

et al. 2006

Drug Metab Dispos

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“…Manipulation of the growth and maturation of SHs is easy, and they can be cryopreserved 17 . Cryopreserved SHs can maintain the abilities of growth and maturation 18 .…”

Section: Anticipated Resultsmentioning

confidence: 99%

Selective proliferation of rat hepatocyte progenitor cells in serum-free culture

et al. 2007

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This protocol details a method of obtaining selectively proliferated hepatocyte progenitor cells using hyaluronic acid (HA)-coated dishes and serum-free medium. A small hepatocyte (SH) is a hepatocyte progenitor cell of adult livers and has many hepatic functions. When the rat SH begins to proliferate, CD44 is specifically expressed. To define the purification of SH, CD44 and cytokeratin 8 are used as marker proteins. The growth of SHs is faster on HA-coated dishes than on other extracellular matrix-coated ones. The use of both DMEM/F12 medium and HA-coated dishes allows the selective proliferation of SHs in culture. The purification of SHs is approximately 85% at day 10.

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“…Compared to typical hepatocytes, these cells have a smaller size and flat shape. Small hepatocytes can proliferate and differentiate to mature hepatocytes in vitro and in vivo [Ikeda et al, 2002;Sidler Pfandler et al, 2004]. These cells were considered 'committed progenitor cells' capable of differentiating into mature hepatocytes when liver repair is urgently needed [Mitaka et al, 1998].…”

Section: Adult Liver Stem/precursor Cellsmentioning

confidence: 99%

Liver Regenerative Medicine: Advances and Challenges

Chistiakov

2012

Cells Tissues Organs

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Liver transplantation is the standard care for many end-stage liver diseases. However, donor organs are scarce and some people succumb to liver failure before a donor is found. Liver regenerative medicine is a special interdisciplinary field of medicine focused on the development of new therapies incorporating stem cells, gene therapy and engineered tissues in order to repair or replace the damaged organ. In this review we consider the emerging progress achieved in the hepatic regenerative medicine within the last decade. The review starts with the characterization of liver organogenesis, fetal and adult stem/progenitor cells. Then, applications of primary hepatocytes, embryonic and adult (mesenchymal, hematopoietic and induced pluripotent) stem cells in cell therapy of liver diseases are considered. Current advances and challenges in producing mature hepatocytes from stem/progenitor cells are discussed. A section about hepatic tissue engineering includes consideration of synthetic and natural biomaterials in engineering scaffolds, strategies and achievements in the development of 3D bioactive matrices and 3D hepatocyte cultures, liver microengineering, generating bioartificial liver and prospects for fabrication of the bioengineered liver.

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Proliferation of rat small hepatocytes after long-term cryopreservation

Cited by 35 publications

References 32 publications

Cytochrome P450 Expression of Cultured Rat Small Hepatocytes after Long-Term Cryopreservation

Cytochrome P450 Expression of Cultured Rat Small Hepatocytes after Long-Term Cryopreservation

Selective proliferation of rat hepatocyte progenitor cells in serum-free culture

Liver Regenerative Medicine: Advances and Challenges

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