Proliferation of the turkey myogenic satellite cell in a serum-free medium

McFarland, Douglas C.; Pesall, Jane E.; Norberg, Jay M.; Dvoracek, Marc A.

doi:10.1016/0300-9629(91)90252-8

Cited by 39 publications

(16 citation statements)

References 22 publications

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“…However, such localization was particularly observed in slowly proliferative SC-injected muscle. Scale bars 50 μm could be observed among different strains within animal species (McFarland et al 1991;Duclos et al 1996) with regard to their response to growth factors and their quantity of growth factor receptors (McFarland et al 1995b). Finally, other studies were based on the SC behavior in vivo after total muscle denervation (Bakou et al 1996) and on their skeletal muscle embryological origin (Pavlath et al 1998).…”

Section: Number Of Nuclei In Chimeric Myotubesmentioning

confidence: 96%

Muscle satellite cell heterogeneity: in vitro and in vivo evidences for populations that fuse differently

et al. 2004

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During development, muscle growth results from the proliferation of satellite cells (SC) and their fusion with fibers. Several studies revealed heterogeneity of SC population notably based on the proliferation rate. Here, we examined the SC characteristics of turkey skeletal muscles in terms of proliferation and more specifically fusion, to define if the ability of these cells to fuse may represent a distinct characteristic between them and could be directly associated with their proliferation properties. Freshly extracted SC were plated in clonal condition and their proliferation rate was assessed 11 days later. To investigate the SC fusion behavior, in vitro and in vivo approaches were developed. Highly and slowly proliferative SC were initially labeled with a nuclear beta-galactosidase (beta-Gal) activity and co-cultured with differentiated primary cultures. After 5 days, distribution of beta-Gal positive (beta-Gal+) nuclei was examined. Also, the two labeled SC types were transplanted into different muscles in autologous model. One week later, number of beta-Gal+ nuclei per fiber and diameter of fibers displaying beta-Gal+ nuclei were determined. In vitro, we showed that SC from turkey skeletal muscle are present as a heterogeneous population in terms of proliferation. Examination of their fusion properties in vitro as well as in vivo revealed that highly proliferative SC exclusively exhibited fusion with differentiated myotubes or myofibers, whereas slowly proliferative SC mainly fused together. Collectively, these data demonstrate for the first time that SC with different proliferation rate also intrinsically differ in their fusion potential, suggesting distinct roles for these sub-populations in muscle growth.

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Section: Number Of Nuclei In Chimeric Myotubesmentioning

confidence: 96%

Muscle satellite cell heterogeneity: in vitro and in vivo evidences for populations that fuse differently

et al. 2004

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“…Serum-free media reduce operating costs and process variability while lessening the potential source of infectious agents (Froud 1999). Improvements in the composition of commercially available cell culture media have enhanced our ability to successfully culture many types of animal cells and serum-free media have been developed to support in vitro myosatellite cell cultures from the turkey (McFarland et al 1991), sheep (Dodson and Mathison 1988) and pig (Doumit et al 1993). Variations among different serum-free media outline the fact that satellite cells from different species have different requirements and respond differentially to certain additives (Dodson et al 1996).…”

Section: Tissue Culturementioning

confidence: 99%

Prospectus of cultured meat—advancing meat alternatives

2010

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“…The highest percent fusion (3%, P < 0.05) displayed by wapiti SC exposed to ITT-CF was considerably lower than the fusion percentage of sheep (35-80%; Dodson et al 1987;Vierck et al 1995), turkey (80-90%;McFarland et al 1991), horse (20-60%; Erickson et al 1998) and fish (60-90%; Mulvaney and Cyrino 1995) primary SC cultures exposed to differentiative treatments. The reason ITT and ITT-CF promoted some fusion, whereas other differentiation procedures did not, is not known.…”

Section: Media Characterizationmentioning

confidence: 99%

Isolation and culture of wapiti (Cervus elaphus) satellite cells

Burton¹,

Shipley²,

Byrne³

et al. 2000

Can. J. Anim. Sci.

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[303][304][305][306][307][308][309]. Myogenic satellite cells (SC) were isolated from the sternomandibularis muscles of two, 227 kg, male wapiti (Cervus Elaphus) and studied in primary cell culture. Wapiti-derived SC were capable of attaching to culture substrata and following the myogenic program of proliferation and differentiation to form multinucleated myotubes. Wapiti SC attached equally well to pig skin gelatin (PSG), fibronectin (FN), Matrigel ® and plastic (P > 00.05), but cell viability measured at 120 h varied depending on initial substratum type. Pig skin gelatin (0.02% wt vol -1 ) was chosen for the majority of subsequent experimentation for cost efficiency. The greatest amount of wapiti SC proliferation was observed in media containing 10% (vol/vol -1 ) horse serum (HS), 15% HS and 15% fetal bovine serum (FBS) (P > 0.05). Wapiti SC proliferated more when exposed to HS and FBS than to sheep serum (SS) (P < 0.05). No proliferation, differentiation or decrease in cell viability was observed in Dulbecco's Modified Eagle Medium (DMEM) + 1% HS, DMEM + 2% HS, DMEM + 3% HS or DMEM + 4% HS (P > 0.05) after 120 h in vitro. Proliferation of SC was doubled when insulin was added to both 10% HS-and 2% HS-containing media (P < 0.05). Although insulin alone in serum-containing media did not promote fusion of wapiti SC, two defined media (ITT and ITT-CF) that contain insulin did promote fusion of wapiti SC cultures. ITT-CF induced 3% fusion of wapiti SC into myotubes, and ITT induced 1% (P < 0.05). There was also an increase in total cell numbers in SC exposed to ITT-CF in comparison with ITT, ovine defined media (ODM) or ovine defined media-Modified (ODM-Mod) )(P < 0.05). Although defined media differed in their ability to induce proliferation or differentiation (P < 0.05), the substrata on which the SC were plated did not influence the defined media effect on SC activity (P > 0.05). Satellite cells exposed to ITT and ITT-CF differed morphologically from SC exposed to ODM and ODM-Mod, which may suggest that formulation differences are influencing wapiti-derived SC proliferation and differentiation. Des cellules satellites (CS) myogènes isolées du muscle sternomandibularis de deux wapitis mâles (Cervus elaphus) de 227 kg ont été examinées en culture primaire. Les cellules étaient capables de se fixer sur les substrats de culture et, après une phase myogène de prolifération et de différenciation, de former des myotubes plurinucléés. Des cellules adhéraient tout aussi bien à la gélatine de peau de porc (GPP), à la fibronectine (FN), au Matrigel ® et au plastique (P > 0,05), mais leur viabilité après 120 h variait selon le type initial de substrat. C'est la gélatine de peau de porc (0,02 % en poids/volume) qui, pour des raisons de rendement économique, a été retenue pour la majorité des essais subséquents. La prolifération la plus abondante des cellules était obtenue dans des milieux de culture contenant 10 % ou 15 % en volume de sérum de cheval (SCh), et 15 % de sérum de bovin fétal (SBF), P > 0,05. La prolifération...

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Proliferation of the turkey myogenic satellite cell in a serum-free medium

Cited by 39 publications

References 22 publications

Muscle satellite cell heterogeneity: in vitro and in vivo evidences for populations that fuse differently

Muscle satellite cell heterogeneity: in vitro and in vivo evidences for populations that fuse differently

Prospectus of cultured meat—advancing meat alternatives

Isolation and culture of wapiti (Cervus elaphus) satellite cells

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