Proliferation patterns of cervical cells as visualized in Leiden liquid cytology slides

BackgroundAlthough liquid-based cytology (LBC) is now recommended for cervical cancer screening, it requires expensive automated devices and materials. To evaluate the efficiency of inexpensive LBC methods relying on an inexpensive fixative liquid, Easyfix®, we compared the results obtained by the liquid-based cytology (LBC) diagnoses performed by cytocentrifugations (Papspin® and Turbitec®) with those obtained by histology. Furthermore, we evaluated the efficiency of the fixative liquid, Easyfix®, to preserve HPV DNA in the collected samples.Method266 LBC were compared with 174 colposcopies and 91 Loop Electrosurgical Excision Procedure (LEEP). Among the LBC, 51 were performed using the Papspin® system and 215 were performed using the Turbitec® system. To control the quality of the preservation liquid, Easyfix®, we correlated the results of HCII assays with those of HPV PCR.ResultsFor Papspin® and Turbitec® systems, the sensitivities were respectively 82.6% (95% CI: 61.2–95.0%, p < 0.001) and 75.0% (95% CI: 64.4–89.8%, p < 0.001) and the specificities were 92.6% (95%CI: 76.5–99.1%, p < 0.001) and 96.2% (95% CI: 91.3–98.7%, p < 0.001). We find no statistical difference between the results of the both systems (p = ns). The sensitivity of the HCII was 86.4% (95% IC: 77.4–92.8%, p < 0.001) and the specificity was 39.4% (95% CI: 31.2–48.1%, p < 0.001). The comparison between HCII and HPV-PCR shows a good correlation: the kappa was 0.89.ConclusionLBC performed by cytocentrifugations are inexpensive, reduce inadequate smears, show excellent efficiency and allow HPV detection by molecular biology.

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“…One main advantage of LBC is that they allow ancillary techniques such as those used in immunocytochemistry or molecular biology [28-32]. …”

Section: Discussionmentioning

confidence: 99%

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Garbar¹,

Mascaux²,

Fontaine³

2005

Cytojournal

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“…[20][21][22] That is an attractive feature and applies also to BoonFix ® samples. 23 Alves et al 24 reported that in spite of different methodologies, the 2 automated, commercial American methods (SurePath ® and ThinPrep ® ) and a manual Brazilian system (DNA-Citoliq, Digene Brazil, São Paulo, Brazil) result in adequately preserved cells, allowing precise morphologic evaluation. This indicates that the direct fixation of the cells after sampling is the most important factor in the morphology of thin-layer slides.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic Parameters in Liquid-Based Cervical Cytology Using a Coagulant Suspension Fixative

Boon¹,

Ouwerkerk‐Noordam²,

Suurmeijer³

et al. 2005

Acta Cytologica

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“…The sensitivities of the conventional Pap smear and LBC tests are estimated to be 70% -80% and 85% -95%, respectively [9]. LBC is currently recommended for cervical cancer screening [10] with a major advantage of allowing ancillary techniques such as those used in immunocytochemistry and molecular biology [11][12][13][14][15][16][17]. Cell blocks made from cell or tissue remnants of LBC can also be used for immunocytochemistry or immunohistochemistry of specific biomarkers to accurate diagnosis of malignancy [16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Use of Liquid-Based Cytology (LBC) and Cell Blocks from Cell Remnants for Cytologic, Immunohistochemical, and Immunocytochemical Diagnosis of Malignancy

Sakamoto¹,

Takenaka²,

Ushimaru³

et al. 2012

OJPathology

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Great advances in screening have lowered the death rate from cervical cancer in the advanced countries. The major advances in cervical cancer screening include the Papanicolaou (Pap) test and liquid-based cytology (LBC). In this study, we aimed to use cell remnants from LBC specimens from uterine cervix and endometrium, aspirates from breast and thyroid tumors, and liquid samples (ascites, pleural effusion, and urine). Cell blocks made from cell remnants of LBC specimens were immunohistochemically or immunocytochemically stained for several biomarkers including certain tumor markers such together with hematoxylin and eosin staining for accurate diagnosis of malignancies in different samples. The findings from the cell blocks stained with these biomarkers combined with those from Pap stain led to easily diagnosis of the presence or absence of malignancies. Our findings suggest the utility of LBC and cell blocks from cell remnants in cytologic diagnosis in certain specimens.

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Proliferation patterns of cervical cells as visualized in Leiden liquid cytology slides

Cited by 7 publications

References 19 publications

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Diagnostic Parameters in Liquid-Based Cervical Cytology Using a Coagulant Suspension Fixative

Use of Liquid-Based Cytology (LBC) and Cell Blocks from Cell Remnants for Cytologic, Immunohistochemical, and Immunocytochemical Diagnosis of Malignancy

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