Proliferative Activity and Viability of Fibroblast and Glioblastoma Cell on Various Types of Carbon Nanotubes

Bobrinetskii, I. I.; Morozov, R. A.; Seleznev, A. S.; Podchernyaeva, R. Ya.; Lopatina, Olga

doi:10.1007/s10517-012-1690-z

Cited by 5 publications

(3 citation statements)

References 10 publications

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“…However, this reduced cell survival ratio was in contrast to the results of Tilton et al [ 44 ], who postulated a MWCNT-dependent regulation of pathways that increase cell proliferation. In turn, Bobrinetskii et al [ 45 ] showed a decreased proliferative activity of human embryo fibroblast and glioblastoma cells cultivated on various types of carbon nanotubes. R28 gene expression analysis revealed an increased downregulation in CDH2 expression after cultivation on MWCNT-covered silicon wafer slices, which may be an explanation for the reduced cell growth.…”

Section: Discussionmentioning

confidence: 99%

Properties of Retinal Precursor Cells Grown on Vertically Aligned Multiwalled Carbon Nanotubes Generated for the Modification of Retinal Implant-Embedded Microelectrode Arrays

Johnen

Meissner

Krug

et al. 2016

Journal of Ophthalmology

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Background. To analyze the biocompatibility of vertically aligned multiwalled carbon nanotubes (MWCNT), used as nanomodification to optimize the properties of prostheses-embedded microelectrodes that induce electrical stimulation of surviving retinal cells. Methods. MWCNT were synthesized on silicon wafers. Their growth was achieved by iron particles (Fe) or mixtures of iron-platinum (Fe-Pt) and iron-titanium (Fe-Ti) acting as catalysts. Viability, growth, adhesion, and gene expression of L-929 and retinal precursor (R28) cells were analyzed after nondirect and direct contact. Results. Nondirect contact had almost no influence on cell growth, as measured in comparison to reference materials with defined levels of cytotoxicity. Both cell types exhibited good proliferation properties on each MWCNT-coated wafer. Viability ranged from 95.9 to 99.8%, in which better survival was observed for nonfunctionalized MWCNT generated with the Fe-Pt and Fe-Ti catalyst mixtures. R28 cells grown on the MWCNT-coated wafers showed a decreased gene expression associated with neural and glial properties. Expression of the cell cycle-related genes CCNC, MYC, and TP53 was slightly downregulated. Cultivation on plasma-treated MWCNT did not lead to additional changes. Conclusions. All tested MWCNT-covered slices showed good biocompatibility profiles, confirming that this nanotechnology is a promising tool to improve prostheses bearing electrodes which connect with retinal tissue.

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Section: Discussionmentioning

confidence: 99%

Properties of Retinal Precursor Cells Grown on Vertically Aligned Multiwalled Carbon Nanotubes Generated for the Modification of Retinal Implant-Embedded Microelectrode Arrays

Johnen

Meissner

Krug

et al. 2016

Journal of Ophthalmology

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“…The authors also argued that the potential genotoxicity of these NPs could be attributed to the particle shape. 66 On the other hand, Bobrinetskii et al 67 examined the effect of SWCNTs on cell viability and proliferation of human embryo fibroblasts and glioblastoma cells. They found that SWCNTs have a low cytotoxic activity on these cells.…”

Section: Swcnts and Mammalian Embryomentioning

confidence: 99%

Impact of single-walled carbon nanotubes on the embryo: a brief review

Moustafa¹,

Mfoumou²,

Roman³

et al. 2016

IJN

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Carbon nanotubes (CNTs) are considered one of the most interesting materials in the 21st century due to their unique physiochemical characteristics and applicability to various industrial products and medical applications. However, in the last few years, questions have been raised regarding the potential toxicity of CNTs to humans and the environment; it is believed that the physiochemical characteristics of these materials are key determinants of CNT interaction with living cells and hence determine their toxicity in humans and other organisms as well as their embryos. Thus, several recent studies, including ours, pointed out that CNTs have cytotoxic effects on human and animal cells, which occur via the alteration of key regulator genes of cell proliferation, apoptosis, survival, cell–cell adhesion, and angiogenesis. Meanwhile, few investigations revealed that CNTs could also be harmful to the normal development of the embryo. In this review, we will discuss the toxic role of single-walled CNTs in the embryo, which was recently explored by several groups including ours.

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“…Numerous studies have been devoted to analysis of the toxicity of CNTs to mammal cells, but the results obtained remain controversial, which may be related with the different methods of obtaining CNTs and dif ferent degrees of their purification (Veetil and Ye, 2009;Wang et al, 2011;Bobrinetskii et al, 2012Bobrinetskii et al, , 2013Alarifi et al, 2014;Ramon Azcon et al, 2014;Dong and Ma, 2015).…”

Section: Introductionmentioning

confidence: 99%

Neuronal differentiation of PC12 cells and mouse neural stem cells on carbon nanotube films

et al. 2016

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In development of methods of stimulation of regeneration of nerve tissues and creation of new generation bioelectronic devices, studying the interaction of nerve cells with specially developed scaffolds with different characteristics of the surface within a nanometer range is a necessary stage. Carbon nanotubes (CNTs), flexible graphene films rolled up into nanosized cylindrical tubes, may represent a promising mate rial for making these scaffolds. CNTs were obtained by chemical vapor deposition. Analysis of PC12 cells cul tivated on quartz glasses covered with CNT films using electron and optical microscopy have been performed. It has been demonstrated that CNTs stimulate proliferation and do not inhibit neuronal differentiation of the PC12 cells. The possibility of obtaining neurons differentiated from mouse neural stem cells on quartz glasses covered with the CNT films has been shown. The data obtained indicate the possibility of using CNT films produced by chemical vapor deposition on quartz glasses as an electroconductive substrate for obtaining cells of neural origin and, possibly, mature neurons and studying their functions.

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Proliferative Activity and Viability of Fibroblast and Glioblastoma Cell on Various Types of Carbon Nanotubes

Cited by 5 publications

References 10 publications

Properties of Retinal Precursor Cells Grown on Vertically Aligned Multiwalled Carbon Nanotubes Generated for the Modification of Retinal Implant-Embedded Microelectrode Arrays

Properties of Retinal Precursor Cells Grown on Vertically Aligned Multiwalled Carbon Nanotubes Generated for the Modification of Retinal Implant-Embedded Microelectrode Arrays

Impact of single-walled carbon nanotubes on the embryo: a brief review

Neuronal differentiation of PC12 cells and mouse neural stem cells on carbon nanotube films

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