Proliferative activity of oocytes in multi-oocyte follicles of bovine ovary

Abstract

We characterized the proliferative activity of multi-oocyte follicles with anti-nuclear antigen of proliferating cells (PCNA). Ovaries (n = 12) from heifers were processed for histology. From 789 multi-oocytes follicles observed, only 11 were considered appropriated for immunostaining, since they presented all nuclei of the oocytes clearly visible. All multi-oocyte follicles were positive for PCNA, but some oocytes showed no proliferative activity. We conclude that oocytes in multi-oocyte follicles seem to be in different stages of the cell cycle.

Comparison of Ovarian Morphology and Follicular Disturbances between Two Inbred Strains of Cotton Rats (Sigmodon hispidus)

“…We also evaluated the proliferative activities in the oocytes of SOF, MOF, and DNOs (Figure 7). In mammalian ovaries, although immunoreactivity for PCNA, a cell proliferation marker, was detected in the oocytes of ruminants such as cattle [12] and sheep [34], it was observed in the GCs and theca cells of mice [35]. In HIS/Hiph, oocytes in SOFs and MOFs or DNOs also showed positivity for PCNA regardless of follicular developmental stage (Figure 7a-c and Figure S1a,b, Supplementary Materials).…”