Proliferative and Migration Activity of JEG-3 Trophoblast Cell Line in the Presence of Cytokines

Sokolov, D. N.; Furaeva, K. N.; Степанова, О. И.; Selkov, S. A.

doi:10.1007/s10517-015-3013-7

Cited by 10 publications

(5 citation statements)

References 53 publications

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“…There is a number of IL-1b sources in the uteroplacental contact zone: trophoblast, uNK cells, and others [2]. Previously, we showed IL-1b ability to enforce JEG-3 cells proliferation in 10 U/mL concentration [3]. Here, we observed no cell mortality growth in this concentration while exceeded concentrations lead to trophoblast mortality growth (Figure 1(D)).…”

Section: Discussionsupporting

confidence: 58%

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The uteroplacental contact zone cytokine influence on NK cell cytotoxicity to trophoblasts

Bazhenov

Михайлова

Nikolaenkov

et al. 2020

Gynecological Endocrinology

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Section: Discussionsupporting

confidence: 58%

“…We showed that IFNc in 1000 U/mL concentration enforced trophoblast mortality but this effect could not be spotted for 40 and 400 U/mL concentrations (Figure 1(D)). We have shown earlier that it was in 40 and 400 U/mL concentrations that IFNc enforced JEG-3 cells proliferation [3].…”

Section: Discussionmentioning

confidence: 86%

The uteroplacental contact zone cytokine influence on NK cell cytotoxicity to trophoblasts

Bazhenov

Михайлова

Nikolaenkov

et al. 2020

Gynecological Endocrinology

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“…Both models had the same vasoactive factors being increased by GBS: IL-6 and CXCL-8. Although IL-6 regulation of trophoblast cell migration is still debated ( Champion et al, 2012 ; Sokolov et al, 2015 ), its roles in vascular dysfunction are well-established. IL-6 has increased levels in preeclamptic patients ( Lockwood et al, 2008 ) and promotes thrombogenesis, increased expression of endothelial adhesion molecules, and vascular permeability ( Roldán et al, 2003 ), increased vascular fibrosis, immune cell recruitment, endothelial activation, and its further dysfunction ( Didion, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Uvaol Prevents Group B Streptococcus-Induced Trophoblast Cells Inflammation and Possible Endothelial Dysfunction

Silva

Botelho

et al. 2021

Front. Physiol.

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Group B Streptococcus (GBS) infection during pregnancy is involved in maternal sepsis, chorioamnionitis, prematurity, fetal infection, neonatal sepsis, and neurodevelopmental alterations. The GBS-induced chorioamnionitis leads to a plethora of immune and trophoblast cells alterations that could influence endothelial cells to respond differently to angiogenic mediators and alter placental vascular structure and function in pregnant women. In this context, preventive measures are needed to reduce such dysfunctions. As such, we evaluated the effects of a non-lethal exposure to inactivated GBS on trophoblast cells and chorionic villi explants, and if the treatment with uvaol would mitigate these effects. The concentration of 106 CFU of GBS was chosen since it was unable to reduce the HTR-8/SVneo cell line nor term chorionic villi explant viability. Raman spectroscopy of trophoblast cells showed significant alterations in their biochemical signature, mostly reverted by uvaol. GBS exposure increased HTR-8/SVneo cells IL-1β and IFN-γ production, phagocytosis, oxidative stress, and decreased trophoblast cell migration. The Ea.hy926 endothelial cell line produced angiopoietin-2, CXCL-8, EGF, FGF-b, IL-6, PlGF, sPECAM-1, and VEGF in culture. When co-cultured in invasion assay with HTR-8/SVneo trophoblast cells, the co-culture had increased production of angiopoietin-2, CXCL-8, FGF-b, and VEGF, while reduced sPECAM-1 and IL-6. GBS exposure led to increased CXCL-8 and IL-6 production, both prevented by uvaol. Chorionic villi explants followed the same patterns of production when exposed to GBS and response to uvaol treatment as well. These findings demonstrate that, even a non-lethal concentration of GBS causes placental inflammation and oxidative stress, reduces trophoblast invasion of endothelial cells, and increases CXCL-8 and IL-6, key factors that participate in vascular dysregulation observed in several diseases. Furthermore, uvaol treatment prevented most of the GBS-provoked changes. Hence, uvaol could prevent the harmful effects of GBS infection for both the mother and the fetus.

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“…The levels of the secreted MMP-2 and MMP-9 were significantly elevated by IL-8 treatment, as was the trophoblast expression of integrin α5 and β1, compared to the non-treated cells [ 24 , 26 ]. Furthermore, the viability and proliferation of HTR-8/SVneo cells increased following IL-8 treatment [ 26 , 54 ], as well as HTR-8/SVneo- and JEG-3-cell migration [ 26 , 55 ]. IL-8 has also been designated as one of the key EVT-secreted factors involved in spiral artery remodeling [ 45 ].…”

Section: Il-6 and Il-8 In A Healthy Pregnancymentioning

confidence: 99%

IL-6 and IL-8: An Overview of Their Roles in Healthy and Pathological Pregnancies

Vilotić

Nacka-Aleksić

Pirković

et al. 2022

IJMS

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Interleukin-6 (IL-6) is an acknowledged inflammatory cytokine with a pleiotropic action, mediating innate and adaptive immunity and multiple physiological processes, including protective and regenerative ones. IL-8 is a pro-inflammatory CXC chemokine with a primary function in attracting and activating neutrophils, but also implicated in a variety of other cellular processes. These two ILs are abundantly expressed at the feto-maternal interface over the course of a pregnancy and have been shown to participate in numerous pregnancy-related events. In this review, we summarize the literature data regarding their role in healthy and pathological pregnancies. The general information related to IL-6 and IL-8 functions is followed by an overview of their overall expression in cycling endometrium and at the feto-maternal interface. Further, we provide an overview of their involvement in pregnancy establishment and parturition. Finally, the implication of IL-6 and IL-8 in pregnancy-associated pathological conditions, such as pregnancy loss, preeclampsia, gestational diabetes mellitus and infection/inflammation is discussed.

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Proliferative and Migration Activity of JEG-3 Trophoblast Cell Line in the Presence of Cytokines

Cited by 10 publications

References 53 publications

The uteroplacental contact zone cytokine influence on NK cell cytotoxicity to trophoblasts

The uteroplacental contact zone cytokine influence on NK cell cytotoxicity to trophoblasts

Uvaol Prevents Group B Streptococcus-Induced Trophoblast Cells Inflammation and Possible Endothelial Dysfunction

IL-6 and IL-8: An Overview of Their Roles in Healthy and Pathological Pregnancies

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