Proliferative effects of five traditional Nigerian medicinal plant extracts on human breast and bone cancer cell lines

Engel, Nadja; Falodun, Abiodun; Kragl, Udo

doi:10.1016/j.jep.2011.07.023

Cited by 70 publications

(50 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is because people are more concern about the side effects of several syntetic medicines which can be a toxic. [4][5] The medicinal properties of traditional medicinal plants have been investigated in the recent scientific developments for treatment of many metabolic diseases. The extract of these plants from various places in the world are reported to possess several pharmacological activities including anti-inflammatory, [6][7] antidiabetic, [8][9][10][11] antimalarial, 12 antimicrobial, [13][14] anti TB, 15 anthelmintic activity, 16 anticancer, [17][18] cylindroxanthones A-C (1-3 etc.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Capacity of Some Selected Medicinal Plants in East Nusa Tenggara, Indonesia: The Potential of Sterculia quadrifida R.Br.

Lulan¹,

Fatmawati

Santoso

et al. 2018

FRA

View full text Add to dashboard Cite

Background:Medicinal plants derived natural product such as alkaloids, flavonoids and terpenes. The traditional uses claim that medicinal plants of NTT are potential folk medicine but very little research has been conducted on these plants. Objective: The present study is directed towards evaluating naturally effective antioxidant of twenty-four traditional medicinal plants collected from Kupang, East Nusa Tenggara (NTT) using in vitro models. Method: The antioxidant activities were determined by using the free radical scavenging assays 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2-2"-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). Analysis of total flavonoid and phenolic contents were expressed as Quercetin Equivalent (QE) and Gallic Acid Equivalent (GAE), respectively. Statistical methods are used in data analysis include mean or standard deviation, and regression analysis. Result: Methanol extract of Sterculia quadrifida R. Br. root exhibited the highest DPPH radical scavenging activity with IC 50 value of 3.11μg/mL, followed by Schleichera oleosa and Euphorbia hirta L. extracts with IC 50 values of 10.05 and 10.09 μg/mL, respectively. Methanol extract of S. quadrifida also showed highest ABTS radical scavenging activity with IC 50 value of 7.29 μg/mL, followed by Eugenia jambolana Lam. and Lamea grandus with IC 50 value of 9.15 and 12.29 μg/mL, respectively. S. quadrifida extract showed high flavonoid and phenolic content with 661.85 mg of GAE and 116.84 mg of QE per 100 g of extracts. Conclusion: The present study gives scientific evidences that twenty four samples of NTT plants have high free radical scavenging capacity. Strong radical scavenging activity of these plants especially S. quadrifida could be considered as a potential source of natural antioxidants.

show abstract

Section: Introductionmentioning

confidence: 99%

Antioxidant Capacity of Some Selected Medicinal Plants in East Nusa Tenggara, Indonesia: The Potential of Sterculia quadrifida R.Br.

Lulan¹,

Fatmawati

Santoso

et al. 2018

FRA

View full text Add to dashboard Cite

show abstract

“…In some cases, LC-MS/MS methods are also used for profiling of bioactive components in herbs on a LTQ Orbitrap instrument [142,143].…”

Section: Miscellaneous Compoundsmentioning

confidence: 99%

Recent developments in qualitative and quantitative analysis of phytochemical constituents and their metabolites using liquid chromatography–mass spectrometry

Guo

Chen

et al. 2013

Journal of Pharmaceutical and Biomedical Analysis

166

View full text Add to dashboard Cite

“…Cells suspension was transferred to FACS tubes (BD Biosciences, Franklin Lakes, NJ) and fixed in 70% ethanol for 12 h at À20 C. After washing with PBS, cells were incubated with RNase (1 mg/mL) at 37 C for 30 min. Finally, cells were resuspended in propidium iodide (50 mg/mL) for at least 3 h at þ4 C protected from light until flow cytometric analysis (Engel et al, 2011;Nebe et al, 2006). Flow cytometric measurements were performed on the flow cytometer BD FACSCalibur (BD Biosciences).…”

Section: Flow Cytometric Measurement Of Cell Proliferationmentioning

confidence: 99%

Novel anticancer alkene lactone fromPersea americana

Falodun¹,

Engel

Kragl³

et al. 2013

Pharmaceutical Biology

View full text Add to dashboard Cite

Context: Persea americana Mill (Lauraceae) root bark is used in ethnomedicine for a variety of diseases including cancer. Objective: To isolate and characterize the chemical constituent in P. americana, and also to determine the anticancer property of a new alkene lactone from the root bark of P. americana. Materials and methods: The MCF-7 cells were treated with different concentrations of the pure compound for 48 h. The percentage of cells in the various phases, online monitoring of metabolic changes and integrin receptor expression determined by flow cytometry. Results: One novel alkene lactone (4-hydroxy-5-methylene-3-undecyclidenedihydrofuran-2 (3H)-one) (1) was isolated and characterized using 1D-NMR, 2D-NMR, infrared, UV and MS. At a concentration of 10 mg/mL, significant reduction of proliferation of MCF-7 was induced while MCF-12 A cell was significantly stimulated by 10 mg/mL. The IC 50 value for MCF-7 cells is 20.48 mg/mL. Lower concentration of 1 harbor no significant effect on either MCF-7 or MCF-12A. The apoptotic rates of MCF-7 cells were increased significantly. At the final concentration 10 mg/mL, up to 80% of all breast cancer cells were dead. On the non-tumorigenic cell line MCF-12A, the same concentrations (1 and 10 mg/mL) of compound 1 caused significant enhanced apoptotic rates. A total of 1 mg/mL of 1 caused a decrease of a4-, a6-, b1-and b3-integrin expression. Conclusions: The compound caused a stimulatory effect on non-tumorigenic MCF-12A cells with respect to cell adhesion while tumorigenic MCF-7 cells detached continuously. This is the first report on the anticancer effects of this class of compound.

show abstract

Proliferative effects of five traditional Nigerian medicinal plant extracts on human breast and bone cancer cell lines

Cited by 70 publications

References 21 publications

Antioxidant Capacity of Some Selected Medicinal Plants in East Nusa Tenggara, Indonesia: The Potential of Sterculia quadrifida R.Br.

Antioxidant Capacity of Some Selected Medicinal Plants in East Nusa Tenggara, Indonesia: The Potential of Sterculia quadrifida R.Br.

Recent developments in qualitative and quantitative analysis of phytochemical constituents and their metabolites using liquid chromatography–mass spectrometry

Novel anticancer alkene lactone fromPersea americana

Contact Info

Product

Resources

About