Proliferative responses of cultured rat prostate and human benign prostatic hyperplasia

Mistry, Damu; Buchanan, Lynn; Dattani, Gopal; Weaver, Patrick; Riches, Andrew

doi:10.1002/pros.2990030310

Cited by 9 publications

(6 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, in the present study, an in vitro model of induced DNA synthesis in cultured rat ventral prostate (Mistry et al, 1982;Buchanan & Riches, 1985, 1986 …”

mentioning

confidence: 85%

“…Organ culture Ventral prostate from young adult Wistar rats (4-6 months old) was maintained in serum free and serum-supplemented (5, 10 or 20% foetal calf serum) organ culture for 4 days (Riches et al, 1976;Mistry et al, 1982;Buchanan & Riches, 1985, 1986 …”

Section: Methodsmentioning

confidence: 99%

“…During the final 24h of the culture period, each culture was labelled with 37KBq (ljCi) of '25lododeoxyuridine (I-UdR: specific activity 185 GBq mg-', Amersham) as previously described (Mistry et al, 1982). The explants were then fixed in Bouins fluid, washed in 70% alcohol and weighed (Riches et al, 1976 Figure 1 illustrates the effects of 4 x 10-I M Extracyt, estramustine and oestradiol-17/3 on the proliferative response of rat ventral prostate to 4 x 10-6M testosterone following four days of organ culture in medium containing 0, 5, 10 or 20% foetal calf serum.…”

Section: Lododeovyuridine (I Udr) Uptakementioning

confidence: 99%

“…Using the same procedure, Figure 5 demonstrates the effects of 4 x 10 -9, 4 x 10-7 and 4 x 10 5M Estracyt, Emeyt, estramustine and oestradiol-17/B on the proliferative response to 4 x 10 -9M testosterone, which is the minimum dose of testosterone for maximum stimulation of I-UdR uptake in serum-free cultures of rat ventral prostate (Mistry et al, 1982). Treatment with 4 x 10-9 and 4 x 10 M oestradiol-17(3 reduced the testosterone response to the level of the testosterone-free controls (P < 0.01), whereas equimolar concentrations of the estramustine compounds were less effective.…”

Section: Lododeovyuridine (I Udr) Uptakementioning

confidence: 99%

See 3 more Smart Citations

Testosterone-induced DNA synthesis in cultured rat ventral prostate: effects of estracyt and its derivatives

Buchanan¹,

Ac²

1987

Br J Cancer

View full text Add to dashboard Cite

Summary Testosterone-induced DNA synthesis in cultured rat ventral prostate was used to compare the direct effects of EstracytR and EmcytR with that of their metabolite, estramustine, and their carrier-hormone, oestradiol-17/ on prostatic growth. In serum-supplemented medium (5-20% FCS), all the compounds were equally effective in suppressing testosterone stimulated DNA synthesis which was reduced by between 40-50%, whereas in serum-free medium the estramustine compounds were consistently less effective than oestradiol-17,B. In the presence of 4 x 10 -9M testosterone in serum-free medium, stimulated DNA synthesis was reduced by 15-30% following incubation with 4 x 10-7 M of EstracytR, EmcytR and estramustine and by 60% with 4 x 10-7 M oestradiol-17f3. Thus, none of the estramustine compounds appear to offer any selective advantage over that of oestradiol-17/1 in suppressing prostatic DNA synthesis at the target tissue level.Prostatic cancer is the most common malignancy of the male urogenital tract and a leading cause of death due to cancer in aging men (Chisholm, 1981;Flanders, 1984 (Tew, 1983;Hoisaeter, 1984).In both the rat (Plym-Forshell & Nilsson, 1974;Hoisaeter, 1976aHoisaeter, , 1977 Hoisaeter & Bakke, 1983) and man (PlymForshell et al., 1976;Sandberg, 1983), Estracyt is rapidly dephosphorylated to yield estramustine and its dehydrogenated counterpart, estromustine. These metabolites are selectively retained in the prostate by interactions with estramustine binding protein (Forsgren et al., 1979(Forsgren et al., , 1981Bjork et al., 1982) and subsequent intracellular hydrolysis of these compounds slowly liberates free nitrogen mustard and oestrogen moieties. However, the question remains whether the antiprostatic actions of Estracyt are mediated by the oestrogen moiety, the nitrogen mustard moiety or the intact drug complex itself.While in vivo studies have shown that Estracyt is a more potent inhibitor of rat prostatic DNA synthesis than either its hormone or cytostatic parts (Hoisaeter, 1976b(Hoisaeter, , 1977, such comparisons are limited by the extensive metabolism of Estracyt in vivo. Thus, in the present study, an in vitro model of induced DNA synthesis in cultured rat ventral prostate (Mistry et al., 1982;Buchanan & Riches, 1985, 1986

show abstract

“…Thus, in the present study, an in vitro model of induced DNA synthesis in cultured rat ventral prostate (Mistry et al, 1982;Buchanan & Riches, 1985, 1986 …”

mentioning

confidence: 85%

Section: Methodsmentioning

confidence: 99%

Section: Lododeovyuridine (I Udr) Uptakementioning

confidence: 99%

Section: Lododeovyuridine (I Udr) Uptakementioning

confidence: 99%

See 2 more Smart Citations

Testosterone-induced DNA synthesis in cultured rat ventral prostate: effects of estracyt and its derivatives

Buchanan¹,

Ac²

1987

Br J Cancer

View full text Add to dashboard Cite

show abstract

“…Ventral prostatic tissue from 4-6-month-old Wistar rats was maintained in organ culture for up to six days as previously described [5]. In designated cultures, the explants were placed either onto strips of Millipore filter (0.45 pm) mounted on stainless-steel grids or directly onto the grids or submerged in culture medium.…”

Section: Materials and Methods Organ Culturementioning

confidence: 99%

Proliferative responses of rat ventral prostate: Effects of variations in organ culture media and methodology

Buchanan¹,

Riches²

1986

The Prostate

View full text Add to dashboard Cite

Effects of variations in organ culture media and methodology on the proliferative activity of rat ventral prostate and its response to testosterone were investigated quantitatively by using the incorporation of [125I]-iododeoxyuridine (125I-UdR) to monitor DNA synthesis. In serum-free medium, maximal increases in DNA synthesis occurred when testosterone was introduced during the first 48 hours of the culture. Supplementation of the medium with 5% fetal calf serum did not alter the responses in control or testosterone-treated cultures, whereas the addition of insulin (3 micrograms/ml) alone or in combination with serum promoted cell proliferation in testosterone-free cultures and enhanced the stimulatory effect of testosterone. Unlike grid-supported cultures, suspension cultures of rat ventral prostate exhibited increased proliferative activity both in the presence and absence of testosterone. Thus, variations in experimental procedure may account for differences in the proliferative activity of rat prostate between organ culture studies.

show abstract

150‐kDa Proteins in Dog Serum Bind 1.5‐kDa Growth‐Promoting Factors for Androgen‐Independent Canine Prostatic Epithelial Cells

CHEVALIER,

MCKERCHER,

CHAPDELAINE

1993

Journal of Andrology

View full text Add to dashboard Cite

Fractions obtained by gel filtration or ultrafiltration of dog serum were tested for their mitogenic activity on canine prostatic epithelial cells: two prostatic growth factor (PGF) entities were found, a major one of 150 kDa (PGF‐I) and a minor one of 1.5–2.0 kDa (PGF‐II). Treatment and/or extraction with acetic acid, hydrochloric acid, or acidified‐ethanol of preparations enriched in PGF‐I obtained either by ion‐exchange chromatography, acetone precipitation, or retention by ultrafiltration membrane (cut‐off 30 kDa) resulted, upon gel filtration, in the detection of a mitogenic activity eluting mainly at the position of PGF‐II. Acid hydrolysis and proteolysis of PGF‐II led to a loss of activity. It is proposed that, in dog serum, mitogenic peptides for prostatic epithelial cells of 1.5 kDa (PGF‐II) are found in their free form and/or in association with proteins of 150 kDa (PGF‐I).

show abstract

Proliferative responses of cultured rat prostate and human benign prostatic hyperplasia

Cited by 9 publications

References 19 publications

Testosterone-induced DNA synthesis in cultured rat ventral prostate: effects of estracyt and its derivatives

Testosterone-induced DNA synthesis in cultured rat ventral prostate: effects of estracyt and its derivatives

Proliferative responses of rat ventral prostate: Effects of variations in organ culture media and methodology

150‐kDa Proteins in Dog Serum Bind 1.5‐kDa Growth‐Promoting Factors for Androgen‐Independent Canine Prostatic Epithelial Cells

Contact Info

Product

Resources

About