Proline Accumulation and <i>AtP5CS2</i> Gene Activation Are Induced by Plant-Pathogen Incompatible Interactions in <i>Arabidopsis</i>

Fabro, Georgina; Kovács, Izabella; Pavet, Valeria; Szabados, László; Alvarez, María Elena

doi:10.1094/mpmi.2004.17.4.343

Cited by 268 publications

(211 citation statements)

References 49 publications

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“…The significant negative correlation between disease incidence and L-proline showed that higher L-proline reduced disease incidence. These results support earlier findings (Fabro et al, 2004). However, G x T interaction was not significant and this may limit the genetic improvement of L-proline content in tomato.…”

Section: Discussionsupporting

confidence: 89%

Distribution of organic metabolites after Fusarium wilt incidence in tomato (Solanum lycopersicum L.)

Alsamir¹,

Mahmood²,

Ahmad³

et al. 2017

Aust J Crop Sci

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Tomato is an important vegetable crop worldwide and Fusarium wilt is a significant disease of tomato in many countries. One hundred and fifty three diverse tomato genotypes collected from local and exotic sources were screened for resistance to F. solani in the greenhouse. Significant variation in genotype response to inoculation was observed. The organic metabolite profiles of resistant and susceptible genotypes were assessed to determine the basis of resistance. Significant genetic variation was observed for ABA, malic acid, citric acid, fructose, glucose, sucrose, L-proline and myo-inositol. The disease treatment produced significant changes in fructose, glucose, proline and sucrose and significant genotype-by-treatment interaction was observed for ABA, citric acid, fructose, glucose, malic acid and sucrose, indicating that genetic improvement of these traits is feasible. Disease incidence was strongly associated with citric acid (R 2 = 0.84), sucrose (R 2 = 0.72) and L-proline (R 2 = 0.76). Principal component analysis confirmed that citric acid and L-proline were important in determining plant disease response. Genetic variation for Fusarium wilt resistance can be used to develop new tomato cultivars with improved disease resistance.

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Section: Discussionsupporting

confidence: 89%

Distribution of organic metabolites after Fusarium wilt incidence in tomato (Solanum lycopersicum L.)

Alsamir¹,

Mahmood²,

Ahmad³

et al. 2017

Aust J Crop Sci

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“…Since increased Pro content could be seen in both ggt1-1 and ndpk2 and exogenous H 2 O 2 could also elicit an increase in Pro content, it is unlikely that this phenotype is caused by altered amino acid metabolism in ggt1-1. Instead, it is consistent with the data of Fabro et al (2004) who observed that leaves infiltrated with xanthine and xanthine oxidase to generate ROS also had an up to fourfold increase in Pro content. The increase in Pro content was linearly related to the amount of xanthine oxidase infiltrated into the leaves indicating a dependence of Pro content on ROS production.…”

Section: Increased Ros Levels Stimulate Aba-induced Pro Accumulationsupporting

confidence: 92%

“…One possibility is that the increased Pro levels of ggt1-1 and ndpk2 were caused by altered expression of P5CS2. Fabro et al (2004) have shown that P5CS2 was upregulated by incompatible plant-pathogen interactions that are known to also increase ROS levels. Alternatively, the increased Pro observed in ggt1-1 and ndpk2 could be caused by up regulation of P5CS1 enzyme activity, down regulation of ProDH activity, altered abundance of the P5CS1 or ProDH proteins, altered levels of substrate for Pro synthesis or altered feedback regulation of Pro metabolism.…”

Section: Increased Ros Levels Stimulate Aba-induced Pro Accumulationmentioning

confidence: 99%

Altered ABA, proline and hydrogen peroxide in an Arabidopsis glutamate:glyoxylate aminotransferase mutant

2007

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Plant responses to abiotic stress are determined both by the severity of the stress as well as the metabolic status of the plant. Abscisic acid (ABA) is a key component in integrating these various signals and controlling downstream stress responses. By screening for plants with decreased RD29A:LUC expression, we isolated two alleles, glutamate:glyoxylate transferase1-1 (ggt1-1) and ggt1-2, of a mutant with altered ABA sensitivity. In addition to reduced ABA induction of RD29A, ggt1-1 was altered in ABA and stress regulation of D 1 -pyrroline-5-carboxylate synthase, proline dehydrogenase and 9-cis-epoxycarotenoid dioxygenase 3, which encode enzymes involved in Pro and ABA metabolsim, respectively. ggt1-1 also had altered ABA and Pro contents after stress or ABA treatments while root growth and leaf water loss were relatively unaffected. A light-dependent increase in H 2 O 2 accumulation was observed in ggt1-1 consistent with the previously characterized role of GGT1 in photorespiration. Treatment with exogenous H 2 O 2 , as well as analysis of a mutant in nucleoside diphosphate kinase 2 which also had increased H 2 O 2 content but is not involved in photorespiration or amino acid metabolism, demonstrated that the greater ABA stimulation of Pro accumulation in these mutants was caused by altered H 2 O 2 content as opposed to other metabolic changes. The results suggest that metabolic changes that alter H 2 O 2 levels can affect both ABA accumulation and ABA sensitivity.

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“…Proline accumulation has been observed in dry conditions (Choudhary et al, 2005), high salt concentration (Yoshiba et al, 1995), high light intensity and UV radiation (Saradhi et al, 1995), heavy metals (Schat et al, 1997), oxidative stress (Yang et al, 2009) and biotic stress (Fabro et al, 2004;Haudecoeur et al, 2009).…”

Section: Resultsmentioning

confidence: 99%

The presence of Microlobius foetidus cause changes in the antioxidant defense of Urochloa decumbens?

et al. 2015

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Urochloa decumbens (Stapf) R. D. Webster (Poaceae) is an exotic species with has spread rapidly through the Cerrado area of Pantanal, Mato Grosso do Sul, Brazil. It has covered the soil aggressively turning it into cultivated pastures. Thus, it has become a challenge to protect native areas due its capacity of exclusion of native species. It has been observed that Microlobius foetidus (Jacq.) M.Sousa & G.Andrade species (Fabaceae) shows a dominant pattern over the development of U. decumbens. This work shows that M. foetidus interfere on the natural growth of U. decumbens within 10 m ratio. Between 15 and 20 m, it was observed an increase of Importance Value index (IVI) and Relative cover (RC) values. It was also observed a variation on the antioxidant defense system of U. decumbens within 10m ratio from M. foetidus. The enzymes superoxide dismutase, catalase and peroxidase present higher levels of activity then those found for glutathione reductase. This data indicates that M. foetidus may have an effect on U. decumbens, increase the activity of antioxidant enzymes. This effect probably happens as means to neutralize the toxic effects of the oxygen generated due to the presence of allelochemicals, which increases oxidative stress.Keywords: antioxidant enzymes, signal grass, garlic stick, competition. A presença de

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Proline Accumulation and AtP5CS2 Gene Activation Are Induced by Plant-Pathogen Incompatible Interactions in Arabidopsis

Cited by 268 publications

References 49 publications

Distribution of organic metabolites after Fusarium wilt incidence in tomato (Solanum lycopersicum L.)

Distribution of organic metabolites after Fusarium wilt incidence in tomato (Solanum lycopersicum L.)

Altered ABA, proline and hydrogen peroxide in an Arabidopsis glutamate:glyoxylate aminotransferase mutant

The presence of Microlobius foetidus cause changes in the antioxidant defense of Urochloa decumbens?

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