Proline-Modified UIO-66 as Nanocarriers to Enhance <i>Candida rugosa</i> Lipase Catalytic Activity and Stability for Electrochemical Detection of Nitrofen

Cheng, Yongfa; Lai, Oi‐Ming; Tan, Chin Ping; Panpipat, Worawan; Cheong, Ling‐Zhi; Shen, Cai

doi:10.1021/acsami.0c17134

Cited by 24 publications

(4 citation statements)

References 37 publications

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“…The decreased catalytic activity of the MOF immobilized lipase during recycling test might be due to the following reasons: 1) Degradation of the ZIFs’ structure due to prolong soaking of the immobilized lipase in PBS during hydrolytic experiment resulted in leakage of the enzyme; 2) leakage of the enzyme during the washing step in the recycling test [13] . Catalytic stability of immobilized CRL using biomimetic mineralization was significantly better than that of surface immobilization [14] and channel immobilization [15] as shown in our previous study.…”

Section: Resultssupporting

confidence: 60%

Biomimetic Mineralization of Metal Ion‐Doped Lipase into ZIF‐8 Framework for Enhanced Hydrolytic Activity in Biphasic System

Lin

Cheng

et al. 2022

ChemistrySelect

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Present work encapsulated metal ion dopped‐Candida rugosa lipase (CRL) into ZIF structure through biomimetic mineralization and evaluated hydrolytic activity of the immobilized lipase using a biphasic system. Magnesium ion (Mg2+) doped‐CRL with high hydrolytic activity (237 % of free CRL) was successfully synthesized. Circular dichroism and fluorescence spectroscopy analysis revealed Mg2+ doping has changed the conformation of CRL resulted in enhanced structural stability of CRL. The synthesized CRL/Mg2+@ZIF‐8 had a mesoporous (3.88 nm) structure with good crystallinity. Mg2+ doping increased the catalytic activity, substrates affinity (lower Km of 0.4102 mM) and catalytic stability of immobilized CRL. Catalytic activity of CRL/Mg2+@ZIF‐8 (0.25±0.012 U) was two‐fold higher than that of CRL@ZIF‐8 (0.10±0.008 U). The synthesized CRL/Mg2+@ZIF‐8 was used in biosensing of a protox‐inhibiting herbicides (nitrofen) in fruit samples. CRL/Mg2+@ZIF‐8 can be used for biosensing of nitrofen with wide detection range (0‐50 μM), low detection limit (0.47 μM) and good recovery rate.

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Section: Resultssupporting

confidence: 60%

Biomimetic Mineralization of Metal Ion‐Doped Lipase into ZIF‐8 Framework for Enhanced Hydrolytic Activity in Biphasic System

Lin

Cheng

et al. 2022

ChemistrySelect

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“…MOF NPs are characterized by their high porosity, large surface area and high thermal stability. UiO-66 is a porous zirconium-based MOF with open octahedral and tetrahedral cavities [ 40 ], and it has been developed for many applications, such as drug delivery systems [ 41 – 43 ], biosensors [ 44 , 45 ], gas/heavy metal absorbents [ 33 , 46 ], and biomedicine. In this study, we used UiO-66 as a drug-loading platform by coencapsulating PFA and TPZ, and a PDA coating was added to prevent premature drug leakage.…”

Section: Resultsmentioning

confidence: 99%

Polydopamine-coated UiO-66 nanoparticles loaded with perfluorotributylamine/tirapazamine for hypoxia-activated osteosarcoma therapy

Chen

Feng

et al. 2021

J Nanobiotechnol

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Background Hypoxia is a characteristic of solid tumors that can lead to tumor angiogenesis and early metastasis, and addressing hypoxia presents tremendous challenges. In this work, a nanomedicine based on oxygen-absorbing perfluorotributylamine (PFA) and the bioreductive prodrug tirapazamine (TPZ) was prepared by using a polydopamine (PDA)-coated UiO-66 metal organic framework (MOF) as the drug carrier. Results The results showed that TPZ/PFA@UiO-66@PDA nanoparticles significantly enhanced hypoxia, induced cell apoptosis in vitro through the oxygen-dependent HIF-1α pathway and decreased oxygen levels in vivo after intratumoral injection. In addition, our study demonstrated that TPZ/PFA@UiO-66@PDA nanoparticles can accumulate in the tumor region after tail vein injection and effectively inhibit tumor growth when combined with photothermal therapy (PTT). TPZ/PFA@UiO-66@PDA nanoparticles increased HIF-1α expression while did not promote the expression of CD31 in vivo during the experiment. Conclusions By using TPZ and PFA and the enhanced permeability and retention effect of nanoparticles, TPZ/PFA@UiO-66@PDA can target tumor tissues, enhance hypoxia in the tumor microenvironment, and activate TPZ. Combined with PTT, the growth of osteosarcoma xenografts can be effectively inhibited. Graphic abstract

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“…Besides, Cheng et al immobilized Pseudomonas aeruginosa lipase with UiO-66 and proline-modified UiO-66 as carriers to prepare nitrophenol biosensor. Due to the addition of proline, allosteric activation changed the conformation of enzyme, increasing its catalytic activity and improving the sensor’s electrochemical performance ( Cheng et al, 2021 ). Due to high requirements for immobilized materials in terms of pH and temperature, enzymes are easily inactivated after long-term storage.…”

Section: Application Of Uio-66-based Electrochemical Biosensorsmentioning

confidence: 99%

Research Progress of UiO-66-Based Electrochemical Biosensors

Zhang

et al. 2022

Front. Chem.

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UiO-66, as a member of the MOFs families, is widely employed in sensing, drug release, separation, and adsorption due to its large specific surface area, uniform pore size, easy functionalization, and excellent stability. Especially in electrochemical biosensors, UiO-66 has demonstrated excellent adsorption capacity and response signal, which significantly improves the sensitivity and specificity of detection. However, the existing application research remains in its infancy, lacking systematic methods, and recycling utilization and exclusive sensing of UiO-66 still require further improvement. Therefore, one of the present research objectives is to explore the breakthrough point of existing technologies and optimize the performance of UiO-66-based electrochemical biosensors (UiO-66-EBs). In this work, we summarized current experimental methods and detection mechanisms of UiO-66-EBs in environmental detection, food safety, and disease diagnosis, analyzed the existing problems, and proposed some suggestions to provide new ideas for future research.

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Proline-Modified UIO-66 as Nanocarriers to Enhance Candida rugosa Lipase Catalytic Activity and Stability for Electrochemical Detection of Nitrofen

Cited by 24 publications

References 37 publications

Biomimetic Mineralization of Metal Ion‐Doped Lipase into ZIF‐8 Framework for Enhanced Hydrolytic Activity in Biphasic System

Biomimetic Mineralization of Metal Ion‐Doped Lipase into ZIF‐8 Framework for Enhanced Hydrolytic Activity in Biphasic System

Polydopamine-coated UiO-66 nanoparticles loaded with perfluorotributylamine/tirapazamine for hypoxia-activated osteosarcoma therapy

Research Progress of UiO-66-Based Electrochemical Biosensors

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