Proline status of genetically stable salt-tolerant Brassica juncea L. somaclones and their parent cv. Prakash

Jain, Sunita; Nainawatee, H. S.; Jain, R. K.; Chowdhury, J. B.

doi:10.1007/bf00235357

Cited by 51 publications

(26 citation statements)

References 25 publications

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“…Similar results were also reported by Fougrere et al (1991) in alfalfa. Relatively, salt tolerant plants of Brassica juncea showed higher degree of osmotic adjustment in the leaves and a higher critical point concentration of NaCl, at which the endogenous level of free proline rose sharply, than did the relatively salt sensitive genotypes (Jain et al, 1991). Higher proline accumulation was found in salt tolerant B. juncea plants with better growth than the control (Kirti et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

Response of maize (Zea mays L.) to seed priming with NaCl and salinity stress

Bakht

Shafi²,

Jamal³

et al. 1970

Span. j. agric. res.

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Salinity is one of the biggest limitants for agriculture in semi-arid areas of the world. An experiment was conducted to study the effect of seed priming with 6 dS m–1 NaCl on growth and yield responses of two maize cultivars (Azam and Sarhad yellow) exposed to three levels of salinity (0, 6, 8 dS m–1). Statistical analysis of the data revealed that cultivars, seed priming with saline water (6 dS m–1) and subsequent exposure to salinity stress had a significant (p < 0.05) effect on germination, days to emergence, plant height, shoot fresh weight, shoot dry weight, leaf area, shoot Na+, K+, proline, abscisic acid contents and yield variables. The results suggested that increasing salinity level had a negative effect on the growth and development of both cultivars under study. Analysis of the data also revealed that maize cv Azam performed better than cv. Sarhad yellow when exposed to different levels of salinity. Priming of cv Azam with NaCl resulted in earlier emergence (2 days) and germination rate (31.92%), plant height (12%), shoot proline (950.33 μg g–1 fresh weight) and ABA levels (0.983 and 1.203 μg g–1 fresh weight) and yield (36% than the non-primed treatment). These results suggest that priming of maize seeds with NaCl before sowing induces physiological and biochemical changes, which resulted in better performance when subsequently exposed to different levels of salinity.

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Section: Discussionmentioning

confidence: 99%

Response of maize (Zea mays L.) to seed priming with NaCl and salinity stress

Bakht

Shafi²,

Jamal³

et al. 1970

Span. j. agric. res.

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“…Proline is a stress indicator (Liu and Zhu 1997) increased heavily in plants under salt stress. Based on the literature and our data, proline has intensively accumulated in salt-sensitive plants (Jain et al 1991). In such case, TH321 and TH310 cultivars could be more sensitive than ST10 and ST162 cultivars (Fig.…”

Section: Proline Contentmentioning

confidence: 96%

Case study of a biological control: Geobacillus caldoxylosilyticus (IRD) contributes to alleviate salt stress in maize (Zea mays L.) plants

Abdelkader

Esawy

2011

Acta Physiol Plant

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The inevitable exposure of crop plants to salt stress is a major environmental problem emerged from the presence of excess NaCl radicals in the soil. Handling the problem in maize plants using a biological agent was the main interest of the present study. The non-pathogenic, halophytic, facultative aerobic bacterium Geobacillus caldoxylosilyticus IRD that was isolated from Marakopara pond in the Atoll Tikehau (French Polynesian, 2005) and found tolerant to salt stress until 3.5% NaCl (w/v). An artificial symbiosis was achieved by inoculating Geobacillus sp. into 5-day-old maize cultivars of triple hybrids (321 and 310) and singlet hybrids (10 and 162). Thereafter, maize seedlings were exposed to 350 mmol NaCl for 10 days. The data revealed that Geobacillus sp. had interacted with salinized maize and improved maize overall growth, dry weight and relative water content. Na ? accumulation was six times less and Cl -accumulation was 13 times less in the tips of salinized maize seedlings upon Geobacillus sp. inoculation. Salinized maize without Geobacillus viewed decayed cortical cells of seedlings. In addition, proline content was two times higher in salinized seedlings lacking Geobacillus. Photosynthetic pigments and antioxidant enzymes were significantly regulated upon inoculation. Beyond this study, we presented a novel insight into a possible role of Geobacillus caldoxylosilyticus bacteria in controlling/protecting maize plants against high salt stress.

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“…Selfed seeds of the five R1 generation lines, SR2P1-2 and SR3P2-1, SR3P6-1 and SR3P6-2 derived from in vitro selected SR-2 and SR-3 somaclones, respectively and CP5-2 from a non-selected somaclone CP-5, and the parent variety Prakash of B. juncea were used in this study. The protocols followed for in vitro selection and characterization of salt-tolerant variants have been described earlier (Jain et al, 1990;1991b). Samples consisted of 5-day-old seedlings, leaf tissue of 6-week-old plants and 10-day-old siliquae.…”

Section: Methodsmentioning

confidence: 99%

“…We have selected genetically stable salt-tolerant plants of Indian mustard (Brassica juncea L.), an important oil-seed crop, by screening in vitro the highly morphogenic cotyledon explant cultures on high NaC1 media (Jain et al, 1990;1991a). The selected plants had higher rates of osmoregulation (Jain et al, 1991b) and performed better for biomass, seed yield and oil content as compared to the parent and a non-selected line under salt-stress (Jain et al, 1990). In this communication, we report the saltstress effected changes in electrophoretic polypeptide pattern of seedlings, leaves and siliqua tissue of in vitro selected salt tolerant variants (SR-2 and SR-3), a non-selected somaclonal line (CP-5) and parent cv.…”

Section: Introductionmentioning

confidence: 99%

Salt-tolerance in Brassica juncea L. II. Salt-stress induced changes in polypeptide pattern of in vitro selected NaCl-tolerant plants

et al. 1992

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The effect of salt-stress was studied on SDS-PAGE pattern of polypeptides in seedling, leaf and siliqua tissues of four genetically stable lines (SR2P1-2, SR3P2-1, SR3P6-1 and SR3P6-2) of in vitro selected NaCl-tolerant plants, a non-selected somaclone (CP5-2) and parent variety Prakash of Brassica juncea L. Seedlings were raised in 0, 50, 100 and 150 meq/1 NaC1 solutions and plants were irrigated with nutrient solution with 0, 30, 60 & 90 meq/l NaC1. Salinity induced distinct genotype specific changes in polypeptide pattern of leaf and siliqua tissues, while it had no effect on the polypeptide pattern of seedlings, radicle or hypocotyl tissues in any of the lines. In leaves, at vegetative stage, a high molecular weight protein of 53.2 kD while disappeared at 60 mM and higher NaCI level in cv. Prakash and SR3P2-1, it appeared in SR2P1-2 and CP5-2 only at these higher salt levels and in SR3P6 lines it was present irrespective of stress conditions. Differences were also observed for a 93.8 kD protein which appeared anew under stress in cv. Prakash, CP5-2 and SR2P1-2, while it was absent in SR-3 lines. Intensity of the 57.3 kD protein decreased in cv. Prakash, increased in SR-2 and CP-5 lines whereas remained unchanged in SR-3 lines under salt-stress. In siliquae, salt stress induced the expression of four new polypeptides (56.1-70.8 kD) at 60 mM NaC1 in cv. Prakash, and at 30mM in SR2P1-2, SR3P2-1 and SR3P6-1 lines, while these were present in CP5-2 and SR3P6-2 even in the absence of NaC1 stress.

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Proline status of genetically stable salt-tolerant Brassica juncea L. somaclones and their parent cv. Prakash

Cited by 51 publications

References 25 publications

Response of maize (Zea mays L.) to seed priming with NaCl and salinity stress

Response of maize (Zea mays L.) to seed priming with NaCl and salinity stress

Case study of a biological control: Geobacillus caldoxylosilyticus (IRD) contributes to alleviate salt stress in maize (Zea mays L.) plants

Salt-tolerance in Brassica juncea L. II. Salt-stress induced changes in polypeptide pattern of in vitro selected NaCl-tolerant plants

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