Prologue

Whittaker, Tom

doi:10.1017/cbo9780511693960.002

Cited by 19 publications

(22 citation statements)

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“…Lactate dehydrogenase was chosen as a marker for cytoplasmic contamination, fumarase and citrate synthase as markers for the presence of mitochondria. Acetylcholinesterase was chosen as a marker for external synaptosome membranes, microsomes derived from cholinergic neuronal cell bodies, and cholinergic axonal fragments (WHITTAKER, 1965), butyrylcholinesterase as a marker for glial cell fragments (WHITTAKER and BARKER, 1972). Table 2 indicates that the two nuclear populations appeared to be virtually free of significant contamination with other subcellular components.…”

Section: Ncmentioning

confidence: 99%

Studies on Rna Synthesis in Two Populations of Nuclei From the Mammalian Cerebral Cortex

Thompson

1973

Journal of Neurochemistry

182

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A method is presented for the rapid separation of cell nuclei from the rabbit cerebral cortex into two populations. The first of these consists largely of nuclei with the morphological characteristics of neuronal nuclei, the second almost entirely of nuclei with the morphological characteristics of glial cell nuclei. From studies based upon sensitivity to the toxin α‐amanitin, the ratio of incorporation of different bases, ionic requirements and differential sensitivity to actinomycin D, it is concluded that under both the classical low and high salt conditions described by other workers, two enzymes are active in RNA synthesis. The presence of a third enzyme of low activity cannot be excluded. No qualitative difference in the number of enzymes involved in RNA synthesis in neuronal and glial cell nuclei has been found, but there are quantitative differences in activity between the two nuclear populations.

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Section: Ncmentioning

confidence: 99%

Studies on Rna Synthesis in Two Populations of Nuclei From the Mammalian Cerebral Cortex

Thompson

1973

Journal of Neurochemistry

182

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“…The influences of drugs on the uptake of 5-hydroxytryptamine by nerve-ending particles of rabbit brain stem Sm,-Recent progress in the technique of isolation of nerve-ending particles (synaptosomes) and synaptic vesicles from brain (Whittaker, 1959 ; DeRobertis, Rodriguez de Lores Arnaiz & Pellegrino de Iraldi, 1962) has made it possible to investigate the detailed mechanisms of uptake, binding and release of biogenic amines at the subcellular level. Maynert & Kuriyama (1964) found that nerveending particles or synaptic vesicles of brain, when incubated in a medium containing noradrenaline or 5-hydroxytryptamine (S-HT), can take up these amines from the medium against the concentration gradient, and they suggested that both nerve ending particles and synaptic vesicles possessed a transport system for noradrenaline and 5-HT.…”

mentioning

confidence: 99%

The influences of drugs on the uptake of 5-hydroxytryptamine by nerve-ending particles of rabbit brain stem

Segawa

Kuruma

1968

Journal of Pharmacy and Pharmacology

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“…This may imply that acetylcarbocholine and acetylsilicocholine have to be classified as partial agonists (Table 1). Acetylcarbocholine was studied extensively by Whittaker (1954) for its action on acetylcholinesterase. In a series of related esters this isostere of acetylcholine appeared to be hydrolysed most quickly by the esterase.…”

mentioning

confidence: 99%

Acetylcarbocholine and acetylsilicocholine: Directly or indirectly acting cholinergic spasmogens?

Henderson

Ariëns

Ellenbroek

et al. 1968

Journal of Pharmacy and Pharmacology

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The cholinergic and anticholinergic actions of nitrogen-free isosteres of acetylcholine and benzilylcholine are described. Esters of two kinds of choline analogues, carbocholine and silicocholine, were used. The spasmogenic activity of acetylcarbocholine and acetylsilicocholine on the guinea-pig ileum was identified as an indirect cholinergic action, in contrast to the direct cholinergic action of furtrethonium and the mainly noncholinergic action of barium ions. In addition to this indirect cholinergic action, both esters show a weak anticholinergic and a weak noncompetitive "papaverinelike" spasmolytic activity. The corresponding benzilyl esters, although without an onium group, are relatively potent anticholinergic compounds.

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Prologue

Cited by 19 publications

References 0 publications

Studies on Rna Synthesis in Two Populations of Nuclei From the Mammalian Cerebral Cortex

Studies on Rna Synthesis in Two Populations of Nuclei From the Mammalian Cerebral Cortex

The influences of drugs on the uptake of 5-hydroxytryptamine by nerve-ending particles of rabbit brain stem

Acetylcarbocholine and acetylsilicocholine: Directly or indirectly acting cholinergic spasmogens?

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