Prolongation of Cardiac Xenograft Survival by Double Filtration Plasmapheresis and Ex Vivo Immunoadsorption

Sato, Yuichi; Kimikawa, Masaaki; Suga, Hideiku; Hayasaka, Y; Teraoka, Satoshi; Agishi, Tetsuzo; Ota, Kazuo

doi:10.1097/00002480-199207000-00122

Cited by 4 publications

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“…The survival time of the transplanted heart extended to 240±141 minutes from 3 minutes when the natural antibodies were removed by plasmapheresis and immunoadsorption simultaneously 24). In this report, IgM and IgG were reduced to 93.9%, and 85.5% respectively 24). In our study, IgM and IgG were reduced respectively to 65.3%, 77.4% by plasmapheresis.…”

Section: Discussionsupporting

confidence: 57%

“…In another study,23) survival of the xenotransplanted heart in which IgM and IgG were respectively reduced to 95.9%, 80.2% by plasmapheresis, was prolonged to 107±47 minutes, copared to hearts without plasmapheresis, which failed in 9±5 minutes. The survival time of the transplanted heart extended to 240±141 minutes from 3 minutes when the natural antibodies were removed by plasmapheresis and immunoadsorption simultaneously 24). In this report, IgM and IgG were reduced to 93.9%, and 85.5% respectively 24).…”

Section: Discussionmentioning

confidence: 59%

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Effect of Hyperkalemia and Hemolysis Caused by Hyperacute Rejection on Cardiac Function in Pig to HumanEx VivoXenogeneic Cardiac Perfusion Model

Kim

Lee

et al. 2011

Korean Circ J

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Background and ObjectivesHyperacute rejection (HAR) is a major obstacle to successful xenotransplantation of vascularized organs. This study was conducted to observe the effect of hemolysis of perfused human whole blood on pig heart function, and determine the major risk factors for preservation of xenoperfused cardiac function using ex-vivo pig to human xenogeneic cardiac perfusion model.Materials and MethodsHarvested pig hearts were perfused with normal human whole blood (group 1), two different types of pre-treated human whole blood (group 2: immunoglobulins were depleted by plasmapheresis, group 3: pre-treated with plasmapheresis, GAS914, cobra venom factor (CVF) and steroid), and normal porcine whole blood as control (group 4) for 3 hours.ResultsDuration of heart beat was significantly prolonged in group 2 and group 3. Histological examination showed widespread HAR features but was gradually delayed in groups 2 and 3 compared to group 1. The absolute levels of serum creatine kinase-MB and Troponin I increased gradually, and was lower in group 3. Serum hemoglobin levels were rapidly increased in groups 3 and 4, compared to group 1. Extracellular potassium level increased sharply from the beginning of blood perfusion in groups 1, 2 and 3, compared to group 4.ConclusionPretreatment of human whole blood, including immunoglobulin depletion, CVF and steroid reduced and delayed the destruction of pig myocardium by HAR. However, the increased extracellular potassium levels in groups 1, 2 and 3 reflected that these treatments could not prohibit myocardial injury by HAR.

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Section: Discussionsupporting

confidence: 57%

Section: Discussionmentioning

confidence: 59%