Prolonged detection of Zika virus RNA in urine samples during the ongoing Zika virus epidemic in Brazil

Campos, Renata de Mendonça; Cirne-Santos, Cláudio César; Meira, Guilherme Louzada Silva; Santos, Luana L. R.; Meneses, Marcelo D. de; Friedrich, Johannes; Jansen, Stéphanie; Ribeiro, Mário Sérgio; Cruz, Igor C. da; Schmidt‐Chanasit, Jonas; Ferreira, Daniel

doi:10.1016/j.jcv.2016.02.009

Cited by 99 publications

(57 citation statements)

References 7 publications

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“…This is related to the fact that more than half of the specimens were collected before CDC and Canada's National Microbiology Laboratory (NML) recommended PCR testing of urine, in addition to blood (21). In our study, almost a third of positive urine specimens were detected Ͼ10 days after symptom onset, which confirms previous data showing that virus shedding in urine continues after the resolution of viremia (8,20,22). Bingham et al showed that the urine of 82% of patients with confirmed ZIKV disease tested positive after 5 days from symptom onset, whereas the blood of none of the patients tested positive when it was collected after 5 days from symptom onset (8).…”

Section: Discussionsupporting

confidence: 91%

Evaluation of Altona Diagnostics RealStar Zika Virus Reverse Transcription-PCR Test Kit for Zika Virus PCR Testing

et al. 2017

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With the emerging Zika virus (ZIKV) epidemic, accessible real-time reverse transcription-PCR (rRT-PCR) assays are needed to streamline testing. The commercial Altona Diagnostics RealStar ZIKV rRT-PCR test kit (Altona PCR) has been approved for emergency use authorization by the U.S. FDA. Our aim was to verify the Altona PCR by comparing it to the CDC-designed dual-target ZIKV rRT-PCR reference assay (reference PCR) and describe the demographics of patients tested for ZIKV by rRT-PCR in Ontario, Canada. A large set of clinical specimens was tested for ZIKV by the Altona PCR and the reference PCR. Positive or equivocal specimens underwent PCR and Sanger sequencing targeting the ZIKV NS5 gene. A total of 671 serum specimens were tested by the reference PCR: 58 (8.6%) were positive, 193 (28.8%) were equivocal, and 420 (62.6%) were negative. Ninety percent of the reference PCR-positive patients were tested in the first 5 days after symptom onset. The Altona PCR was performed on 284/671 specimens tested by the reference PCR. The Altona PCR was positive for 53/58 (91%) reference PCR-positive specimens and 16/193 (8%) reference PCR-equivocal specimens; the ZIKV NS5 PCR was positive for all 68 Altona PCR-positive specimens and negative for all 181 Altona PCR-negative specimens that underwent the NS5 PCR. The Altona PCR has very good sensitivity (91%) and specificity (97%) compared to the reference PCR. The Altona PCR can be used for ZIKV diagnostic testing and has less extensive verification requirements than a laboratory-developed test.

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Section: Discussionsupporting

confidence: 91%

Evaluation of Altona Diagnostics RealStar Zika Virus Reverse Transcription-PCR Test Kit for Zika Virus PCR Testing

et al. 2017

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“…In the initial phase of the disease (first seven days) viral nucleic acids can be detected in the serum (Haug et al 2016). Sample involves serum of patients or umbilical cord sample of infants, urine, nasopharyngeal swab, saliva, amniotic fluid, CSF, and frozen and fixed placenta Shinohara et al 2016;Tognarelli et al 2015;Leung et al 2015;Gourinat et al 2015;Balm et al 2012;de M Campos et al 2016). Shedding of virus in different body fluids like urine (persists for 15À20 days) has to be elucidated to establish a better diagnostic assay combined with exact sample of choice (Moulin et al 2016).…”

Section: Diagnosis Surveillance and Monitoringmentioning

confidence: 99%

Zika virus – emergence, evolution, pathology, diagnosis, and control: current global scenario and future perspectives – a comprehensive review

Singh

Dhama

Malik

et al. 2016

Veterinary Quarterly

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This review converses the Zika virus which has attained global concern due to its rapid pandemic potential and impact on humans. Though Zika virus was first isolated in 1947, till the recent large-scale outbreak which occurred in Micronesia, in 2007, the virus was placed into the innocuous pathogen category. The World Health Organization on 1 February 2016 declared it as a 'Public Health Emergency of International Concern.' Of the note, American as well as Pacific Island strains/isolates is relatively closer to Asian lineage strains. The African and American strains share more than 87.5% and 95% homologies with Asian strains/isolates, respectively. Asian strains form independent clusters, except those isolated from China, suggesting relatively more diversity than African strains. Prevention and control are mainly aimed at the vector population (mosquitoes) with Aedes aegypti being the main species. Surveys in Africa and Asia indicated seropositivity in various animal species. However, so far its natural reservoir is unknown. There is an urgent need to understand why Zika virus has shifted from being a virus that caused mild illness to unforeseen birth defects as well as autoimmune-neurological problems. Unfortunately, an effective vaccine is not available yet. Availability of cryo-electron microscopy based on 3.8 A resolution revealing mature Zika virus structure and the probable virus attachment site to host cell would provide critical insights into the development of antiviral treatments and vaccines.

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“…Hastalardan elde edilen idrar (18) , tükrük (4) , anne sütü (24) , amniyotik sıvı (8) , plasental dokularda (52) ZİKV saptanmıştır. ZİKV salgını sırasında mikrosefali olgularında saptanan artış, virüsün perinatal yolla bebeğe bulaşı konusunda endişeleri arttırmış, dikkatlerin bu konu üzerine yoğunlaşmasına neden olmuştur.…”

Section: Si̇vri̇si̇nek Dişi Bulaşunclassified

“…RT-PCR testlerinin tanıda en başarılı olduğu dönem klinik bulgular geliştikten sonraki ilk 7 gündür (66) . Bununla beraber ZİKV RNA'sının idrar, tükrük gibi materyallerde serumdan daha uzun süre pozitif saptanabildiğini ileri süren yayınlar bulunmaktadır (2,10,18) . Fonseca ve ark.…”

Section: Taniunclassified

Novel Global Threat: Zika Virus Infections

Nemli¹,

Demırdal²

2016

Ankem Derg

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Prolonged detection of Zika virus RNA in urine samples during the ongoing Zika virus epidemic in Brazil

Cited by 99 publications

References 7 publications

Evaluation of Altona Diagnostics RealStar Zika Virus Reverse Transcription-PCR Test Kit for Zika Virus PCR Testing

Evaluation of Altona Diagnostics RealStar Zika Virus Reverse Transcription-PCR Test Kit for Zika Virus PCR Testing

Zika virus – emergence, evolution, pathology, diagnosis, and control: current global scenario and future perspectives – a comprehensive review

Novel Global Threat: Zika Virus Infections

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