Prolonged erythrocyte auto-incubation as an alternative model for oxidant generation system

Silva, Danilo Grünig Humberto da; Chaves, Nayara Alves; Miyamoto, Sayuri; Almeida, Eduardo Alves de

doi:10.1016/j.tiv.2019.01.006

Cited by 6 publications

(7 citation statements)

References 86 publications

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“…To accomplish this aim, pharmacological doses of Mel have been employed (µM) in line with what already reported in both in vitro and in vivo investigations [15]. In particular, the choice of µM Mel doses is supported by da Silva and co-authors [19], who recently described a beneficial effect of 100 µM Mel on erythrocytes exposed to oxidative stress, as well as by Venegas and co-authors [30] reporting about the therapeutical effect of Mel administered in rats at a µM dose.…”

Section: Discussionmentioning

confidence: 99%

“…As a second step, 100 µM Mel, pharmacological dose also used in other in vitro studies [19] has been considered. Mel doses higher than 100 µM, though not causing lipid peroxidation, have not been used as out of pharmacological range used in both in vivo and in vitro investigations [15].…”

Section: Discussionmentioning

confidence: 99%

“…Its anti-inflammatory activity, immune enhancement, prevention of carcinogenesis and tumor promotion are already known [13,14], while its antioxidant power remains not completely clarified [15,16], as both antioxidant and pro-oxidant activity are described, depending on concentration and cell target [17,18]. Though plenty of experiments showed that Mel is a powerful free radical scavenger and antioxidant at different extent detected on lipids and proteins [19], other in vitro studies highlight its context specific pro-oxidant actions, namely in cancer cells [14].…”

Section: Introductionmentioning

confidence: 99%

“…In order to understand whether Mel displays anti- or pro-oxidant action on Band 3 protein, lipid peroxidation possibly induced by Mel has been monitored [21]. To this end, pharmacological Mel doses (µM) have been used according to other in vivo and in vitro studies [15,19]. Successively, the Mel antioxidant effect has been verified by exposing erythrocytes to 300 μM H 2 O 2 and then evaluating cell shape alterations, rate constant for SO 4 = uptake through Band 3 protein along with Band 3 protein expression levels [22,23].…”

Section: Introductionmentioning

confidence: 99%

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Melatonin Protects Band 3 Protein in Human Erythrocytes against H2O2-Induced Oxidative Stress

Morabito

Marino

2019

Molecules

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The beneficial effect of Melatonin (Mel), recognized as an anti-inflammatory and antioxidant compound, has been already proven to prevent oxidative stress-induced damage associated to lipid peroxidation. As previous studies modeled the impact of oxidative stress on Band 3 protein, an anion exchanger that is essential to erythrocytes homeostasis, by applying H2O2 at not hemolytic concentrations and not producing lipid peroxidation, the aim of the present work was to evaluate the possible antioxidant effect of pharmacological doses of Mel on Band 3 protein anion exchange capability. The experiments have been performed on human erythrocytes exposed to 300 μM H2O2-induced oxidative stress. To this end, oxidative damage has been verified by monitoring the rate constant for SO4= uptake through Band 3 protein. Expression levels of this protein Mel doses lower than 100 µM have also been excluded due to lipid peroxidation, Band 3 protein expression levels, and cell shape alterations, confirming a pro-oxidant action of Mel at certain doses. On the other hand, 100 µM Mel, not provoking lipid peroxidation, restored the rate constant for SO4= uptake, Band 3 protein expression levels, and H2O2-induced cell shape alterations. Such an effect was confirmed by abolishing the endogenous erythrocytes antioxidant system. Therefore, the present findings show the antioxidant power of Mel at pharmacological concentrations in an in vitro model of oxidative stress not associated to lipid peroxidation, thereby confirming Band 3 protein anion exchange capability measurement as a suitable model to prove the beneficial effect of Mel and support the use of this compound in oxidative stress-related diseases affecting Band 3 protein.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Melatonin Protects Band 3 Protein in Human Erythrocytes against H2O2-Induced Oxidative Stress

Morabito

Marino

2019

Molecules

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“…Among antioxidants introduced by food or adopted in clinic therapy to counteract OS, melatonin (Mel) was considered in the present study [29,30]. Melatonin, a neuroendocrine hormone primarily produced by the pineal gland, has been shown to exert its antioxidant power on lipids and proteins [31], though its antioxidant activity depends on concentration and cell target [32,33].…”

Section: Introductionmentioning

confidence: 99%

High Glucose Concentrations Affect Band 3 Protein in Human Erythrocytes

et al. 2020

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Hyperglycemia is considered a threat for cell homeostasis, as it is associated to oxidative stress (OS). As erythrocytes are continuously exposed to OS, this study was conceived to verify the impact of either diabetic conditions attested to by glycated hemoglobin (Hb) levels (>6.5% or higher) or treatment with high glucose (15–35 mM, for 24 h) on erythrocyte homeostasis. To this aim, anion exchange capability through the Band 3 protein (B3p) was monitored by the rate constant for SO42− uptake. Thiobarbituric acid reactive species (TBARS), membrane sulfhydryl groups mostly belonging to B3p, glutathione reduced (GSH) levels, and B3p expression levels were also evaluated. The rate constant for SO42− uptake (0.063 ± 0.001 min−1, 16 min in healthy volunteers) was accelerated in erythrocytes from diabetic volunteers (0.113 ± 0.001 min−1, 9 min) and after exposure to high glucose (0.129 ± 0.001in−1, 7 min), but only in diabetic volunteers was there an increase in TBARS levels and oxidation of membrane sulfhydryl groups, and a decrease in both GSH and B3p expression levels was observed. A combined effect due to the glycated Hb and OS may explain what was observed in diabetic erythrocytes, while in in vitro hyperglycemia, early OS could explain B3p anion exchange capability alterations as proven by the use of melatonin. Finally, measurement of B3p anion exchange capability is a suitable tool to monitor the impact of hyperglycemia on erythrocytes homeostasis, being the first line of high glucose impact before Hb glycation. Melatonin may be useful to counteract hyperglycemia-induced OS at the B3p level.

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Ginseng polysaccharide attenuates red blood cells oxidative stress injury by regulating red blood cells glycolysis and liver gluconeogenesis

Wang¹,

Zhao²,

Yang³

et al. 2023

Journal of Ethnopharmacology

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Prolonged erythrocyte auto-incubation as an alternative model for oxidant generation system

Cited by 6 publications

References 86 publications

Melatonin Protects Band 3 Protein in Human Erythrocytes against H2O2-Induced Oxidative Stress

Melatonin Protects Band 3 Protein in Human Erythrocytes against H2O2-Induced Oxidative Stress

High Glucose Concentrations Affect Band 3 Protein in Human Erythrocytes

Ginseng polysaccharide attenuates red blood cells oxidative stress injury by regulating red blood cells glycolysis and liver gluconeogenesis

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