Prolonged gene expression in primary porcine pancreatic cells using an Epstein–Barr virus-based episomal vector

An OriP/EBNA‐1‐based baculovirus vector with prolonged and enhanced transgene expression

“…The EBNA‐1 and Ori P sequences used in this report were from a commercial plasmid pCEP4. Previous research reported that pCEP4‐derived vectors mediated prolonged and enhanced transgene expression in mammalian cells 24, 25, usually under selective pressure. However, the expression of EBNA‐1 was not investigated in detail in these works.…”

Liver-directed gene therapy of diabetic rats using an HVJ-E vector containing EBV plasmids expressing insulin and GLUT 2 transporter

“…30 For example, it has been shown that while intravenous or intraportal treatment of STZ-induced diabetic rats with a nonviral vector expressing the insulin gene improved their blood glucose levels, the animals became hyperglycemic again within 1 week. 25,31 The duration of gene expression can be improved by employing the EBVbased plasmid vector in various tissues, 10,11,32,33 even though some reports showed prolonged gene expression without the EBV replicon. 34,35 With regard to insulin gene delivery into liver, recently, Yasutomi et al 33 reported that hydrodynamic-based transfection of diabetic mice liver with naked EBV-based plasmid vectors containing the proinsulin gene resulted in efficient hormone production.…”

“…The application of this plasmid in various cells has been developed. [10][11][12] EBV is of particular interest as a vector because of its latent infection property, which is characterized by autonomous replication and nuclear retention of the EBV genome in host cells. 13 The persistent maintenance of the EBV genome has been analyzed in depth and is mediated by the replication origin of the latent viral DNA (oriP) and EBV nuclear antigen 1 (EBNA-1).…”

Gene Therapy for Type 1 Diabetes

“…Gene vectors which transduce islets Adeno-associated virus [179][180][181][182][183][184][185][186][187][188] Episomal DNA [160][161][162][163][164] Herpes simplex virus 194,195 Adenovirus [165][166][167][168][169][170][171][172][173][174][175][176][177][178] HIV-1 [190][191][192] Cationic liposomes 162,163,173 Baculovirus 196 Peptide fusion domains 21,22,197 MoLV retrovirus 189 Genes that promote islet allo-/xenograft survival in vitro and in vivo and/or beta cell survival in culture Antiapoptotic genes: Cytokines: Immunoregulatory genes: bcl-2 183,185,194,198 bcl-xL 22,199 Heme oxygenase-1 20...…”