Prolonged postsurgical recovery period and adverse effects of a leptin application in endotoxemic obese rodents

Poppendieck, Sonja; Kapapa, Melanie; Haas, Philipp; Kielstein, Heike

doi:10.1016/j.lfs.2013.06.015

Cited by 3 publications

(4 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Animals recovered well from the dual implantation surgery, in agreement with literature reports of normalized behavior within three days of surgery. 16 Basal and pharmacological hemodynamic measures were consistent when assessed chronically over five weeks or during repeated acute administration. Reaction to the injection alone was limited, indicating that the procedure induces minimal stress, allowing accurate physiological data to be obtained from both normal and obese conscious animals.…”

Section: Discussionmentioning

confidence: 86%

“…Obese patients and animals exhibit prolonged post-surgical recovery and a higher incidence of infection. 16,29 Additionally, as with the hypertensive models mentioned above, obesity is associated with augmented hemodynamic responses to multiple stressors. [30][31][32] Therefore, it is important to validate both the instrumentation and experimental procedures of new techniques in target disease states.…”

mentioning

confidence: 97%

“…Acute pharmacological testing of hemodynamic responses in tandem with radio-telemetry has previously been achieved through oral administration, 7,12 repeated cannulation, 9,11 intraperitoneal injection 13,14 or commonly via indwelling intravenous catheters. [15][16][17] While the latter provides the vascular access required for accurate assessment of acute pharmacological responses, open catheters increase the risk of infection and thus are generally only used for short-term investigations requiring patency for only a few days. 15,[18][19][20] This style of catheter also requires the animal to be tethered for the length of the experimental period, 15,20,21 maintaining a degree of restraint.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Dual implantation of a radio-telemeter and vascular access port allows repeated hemodynamic and pharmacological measures in conscious lean and obese rats

et al. 2014

View full text Add to dashboard Cite

Expansion of physiological knowledge increasingly requires examination of processes in the normal, conscious state. The current study describes a novel approach combining surgical implantation of radio-telemeters with vascular access ports (VAPs) to allow repeated hemodynamic and pharmacological measures in conscious rats. Dual implantation was conducted on 16-week-old male lean and obese Zucker rats. Continued viability one month after surgery was observed in 67% of lean and 44% of obese animals, giving an overall 54% completion rate. Over the five-week measurement period, reliable and reproducible basal mean arterial pressure and heart rate measures were observed. VAP patency and receptor-independent vascular reactivity were confirmed by consistent hemodynamic responses to sodium nitroprusside (6.25 µg/kg). Acutely, minimal hemodynamic responses to repeated bolus administration of 0.2 mL saline indicated no significant effect of increased blood volume or administration stress, making repeated acute measures viable. Similarly, repeated administration of the β-adrenoceptor agonist dobutamine (30 µg/kg) at 10 min intervals resulted in reproducible hemodynamic changes in both lean and obese animals. Therefore, our study demonstrates that this new approach is viable for the acute and chronic assessment of hemodynamic and pharmacological responses in both lean and obese conscious rats. This technique reduces the demand for animal numbers and allows hemodynamic measures with minimal disruption to animals' welfare, while providing reliable and reproducible results over several weeks. In conclusion, dual implantation of a radio-telemeter and VAP introduces a valuable technique for undertaking comprehensive studies involving repeated pharmacological tests in conscious animals to address important physiological questions.

show abstract

Section: Discussionmentioning

confidence: 86%

mentioning

confidence: 97%

mentioning

confidence: 99%

See 1 more Smart Citation

Dual implantation of a radio-telemeter and vascular access port allows repeated hemodynamic and pharmacological measures in conscious lean and obese rats

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Heparinized saline (250 units/mL) was injected through the cannula in the second and fourth day to maintain cannula patency (Martin et al, 1996). Post-surgery, animals were housed individually and cardiovascular recordings were performed 4 days after the surgery (Poppendieck et al, 2013).…”

Section: Methodsmentioning

confidence: 99%

Moderate Physical Training Ameliorates Cardiovascular Dysfunction Induced by High Fat Diet After Cessation of Training in Adult Rats

et al. 2019

View full text Add to dashboard Cite

We aimed to test whether moderate physical training can induce long-lasting protection against cardiovascular risk factors induced by high fat diet (HFD) intake, even after cessation of training. 90-days-old Wistar rats were submitted to a sedentary lifestyle or moderate physical training, three times a week, for 30 days. Following this, at 120 days-of age, sedentary and trained rats received a hypercaloric diet (HFD) or a commercial diet normal fat diet (NFD) for 30 days. Body weight (BW) and food intake were evaluated weekly. At 150 days-of age, hemodynamic measures (systolic, diastolic, mean blood pressure, pulse pressure, pulse interval and heart rate) were made via an indwelling femoral artery catheter. Beat-to-beat data were analyzed to calculate power spectra of systolic blood pressure (SBP) and pulse interval. After euthanasia, mesenteric fat pads were removed and weighted and total blood was stored for later analysis of lipid profile. Consumption of a HFD increased blood pressure (BP), pulse pressure, low frequency BP variability, BW gain, fat pad stores and induced dyslipidemia. Interestingly, prior physical training was able to partially protect against this rise in BP and body fat stores. Prior physical training did not totally protect against the effects of HFD consumption but previously trained animals did demonstrate resistance to the development of cardiometabolic alterations, which illustrate that the benefits of physical training may be partially maintained even after 30 days of detraining period.

show abstract

Prolonged High Fat Diet Worsens the Cellular Response to a Small, Covert-like Ischemic Stroke

et al. 2019

View full text Add to dashboard Cite

Prolonged postsurgical recovery period and adverse effects of a leptin application in endotoxemic obese rodents

Cited by 3 publications

References 55 publications

Dual implantation of a radio-telemeter and vascular access port allows repeated hemodynamic and pharmacological measures in conscious lean and obese rats

Dual implantation of a radio-telemeter and vascular access port allows repeated hemodynamic and pharmacological measures in conscious lean and obese rats

Moderate Physical Training Ameliorates Cardiovascular Dysfunction Induced by High Fat Diet After Cessation of Training in Adult Rats

Prolonged High Fat Diet Worsens the Cellular Response to a Small, Covert-like Ischemic Stroke

Contact Info

Product

Resources

About