Prolonged transendothelial migration of human haematopoietic stem and progenitor cells (HSPCs) towards hydrogel-released SDF1

Sobkow, Lidia; Seib, F. Philipp; Prodanov, Ljupco; Kurth, Ina; Drichel, Juliane; Bornhäuser, Martin; Werner, Carsten

doi:10.1007/s00277-011-1155-x

Cited by 10 publications

(3 citation statements)

References 46 publications

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“…For example, stromal derived factor 1 (SDF‐1), expressed by tissue‐resident cells, can guide stem cell homing and migration at the injured site 221, 222. Taking advantage of the high affinity interaction between heparan sulfate and SDF‐1, heparin‐cross linked collagen gels223 and starPEG‐heparin hydrogels224 retained SDF‐1 and significantly enhanced progenitor cell migration within the functionalized hydrogels. In addition, collagen‐based matrices loaded with SDF‐1 encapsulated within alginate microspheres enhanced the recruitment of bone marrow derived cells and local CXCR4 + cells in a murine model of ischemic skeletal muscle 225.…”

Section: Engineering the Regenerative Microenvironment With Signalmentioning

confidence: 99%

Engineering the Regenerative Microenvironment with Biomaterials

Rice¹,

Martino²,

Laporte³

et al. 2012

Adv Healthcare Materials

355

310

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Modern synthetic biomaterials are being designed to integrate bioactive ligands within hydrogel scaffolds for cells to respond and assimilate within the matrix. These advanced biomaterials are only beginning to be used to simulate the complex spatio-temporal control of the natural healing microenvironment. With increasing understanding of the role of growth factors and cytokines and their interactions with components of the extracellular matrix, novel biomaterials are being developed that more closely mimic the natural healing environments of tissues, resulting in increased efficacy in applications of tissue repair and regeneration. Herein, the important aspects of the healing microenvironment, and how these features can be incorporated within innovative hydrogel scaffolds, are presented.

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Section: Engineering the Regenerative Microenvironment With Signalmentioning

confidence: 99%

Engineering the Regenerative Microenvironment with Biomaterials

Rice¹,

Martino²,

Laporte³

et al. 2012

Adv Healthcare Materials

355

310

View full text Add to dashboard Cite

show abstract

“…The presentation of these signals in an HSC culture system can be precisely controlled using biomaterial scaffolds. In fact, biomaterial scaffolds have already been designed to precisely control the presentation of growth factors (e.g., stem cell factor (SCF), stromal cell-derived factor 1 (SDF1) 18 19 , cell surface ligands (e.g., cadherins, Jagged1) 20 21 , ECM components (e.g., fibronectin (FN), heparan sulfate) 22 and topographical features 23 24 to recapitulate the bone marrow (BM) microenvironment in vitro. These modular toolboxes along with in-depth in vitro analysis provide tools to facilitate the understanding of autocrine and paracrine signaling in HSC regulation.…”

mentioning

confidence: 99%

Distinguishing autocrine and paracrine signals in hematopoietic stem cell culture using a biofunctional microcavity platform

Müller

Wang

Qiao

et al. 2016

Sci Rep

Self Cite

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Homeostasis of hematopoietic stem cells (HSC) in the mammalian bone marrow stem cell niche is regulated by signals of the local microenvironment. Besides juxtacrine, endocrine and metabolic cues, paracrine and autocrine signals are involved in controlling quiescence, proliferation and differentiation of HSC with strong implications on expansion and differentiation ex vivo as well as in vivo transplantation. Towards this aim, a cell culture analysis on a polymer microcavity carrier platform was combined with a partial least square analysis of a mechanistic model of cell proliferation. We could demonstrate the discrimination of specific autocrine and paracrine signals from soluble factors as stimulating and inhibitory effectors in hematopoietic stem and progenitor cell culture. From that we hypothesize autocrine signals to be predominantly involved in maintaining the quiescent state of HSC in single-cell niches and advocate our analysis platform as an unprecedented option for untangling convoluted signaling mechanisms in complex cell systems being it of juxtacrine, paracrine or autocrine origin.

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“…Several experimental techniques have been introduced for this purpose because many more factors and environmental conditions are involved in the migration of HSPCs than in the migration of other cells. Sobkow et al [9] attempted to regulate the migration of HSPCs by mimicking the endothelial lining of the BM using a Transwell, human umbilical vascular endothelial cells, and a hydrogel containing stromal cell-derived factor 1 (SDF-1). De Barros et al [10] constructed a spheroid with mesenchymal stem cells (MSCs) or osteoblasts (OBs), which are involved in the homing of HSPCs in the BM, to study the movement of HSPCs by simulating the BM niche.…”

Section: Introductionmentioning

confidence: 99%

A Platform for Studying of the Three-Dimensional Migration of Hematopoietic Stem/Progenitor Cells

Lee

Kim

Kang

et al. 2019

Tissue Eng Regen Med

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BACKGROUND: Hematopoietic stem/progenitor cells (HSPCs) have the property to return to the bone marrow, which is believed to be critical in situations such as HSPC transplantation. This property plays an important role in the stemness, viability, and proliferation of HSPCs, also. However, most in vitro models so far have not sufficiently simulated the complicate environment. Here, we proposed a three-dimensional experimental platform for the quantitative study of the migration of HSPCs. METHODS: After encapsulating osteoblasts (OBs) in alginate beads, we quantified the migration of HSPCs into the beads due to the physical environment using digital image processing. Intermittent hydrostatic pressure (IHP) was used to mimic the mechanical environment of human bone marrow without using any biochemical factors. The expression of stromal cellderived factor 1 (SDF-1) under IHP was measured. RESULTS: The results showed that the presence of OBs in the hydrogel scaffold initiate the movement of HSPCs. Furthermore, the IHP promotes the migration of HSPCs, even without the addition of any biochemical factors, and the results were confirmed by measuring SDF-1 levels. CONCLUSION: We believe this suggested three-dimensional experimental platform consisting of a simulated in vivo physical environment and encapsulated OBs should contribute to in vitro migration studies used to investigate the effects of other external factors.

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Prolonged transendothelial migration of human haematopoietic stem and progenitor cells (HSPCs) towards hydrogel-released SDF1

Cited by 10 publications

References 46 publications

Engineering the Regenerative Microenvironment with Biomaterials

Engineering the Regenerative Microenvironment with Biomaterials

Distinguishing autocrine and paracrine signals in hematopoietic stem cell culture using a biofunctional microcavity platform

A Platform for Studying of the Three-Dimensional Migration of Hematopoietic Stem/Progenitor Cells

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