Prolonging the longevity of <i>ex situ</i> conserved seeds by storage under anoxia

Groot, S.P.C.; Groot, L. de; Kodde, Jan; Treuren, R. van

doi:10.1017/s1479262114000586

Cited by 105 publications

(82 citation statements)

References 40 publications

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“…Seed WC appears to be decisive for seed responsivity to O 2 during ageing. At higher WC O 2 may promote seed longevity by allowing O 2 -mediated metabolism, whereas decreasing O 2 levels increases seed survival at slower ageing rates (3,5,37,40). The minor effects of O 2 levels on viability loss observed here (Fig.…”

Section: Discussionmentioning

confidence: 66%

Abscisic acid-determined seed vigour differences do not influence redox regulation during ageing

Schausberger

Roach

Stöggl

et al. 2019

Biochemical Journal

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word count = 187)High seed quality is a key trait to achieve successful crop establishment required for optimum yield and sustainable production. Seed storage conditions greatly impact on two key seed quality traits; seed viability (ability to germinate and produce normal seedlings) and vigour (germination performance). Accumulated oxidative damage accompanies loss of seed vigour and viability during ageing, indicating that redox control is key to longevity. Here, we studied the effects of controlled deterioration at 40 °C and 75% relative humidity ("ageing") under two different O 2 concentrations (21 and 78% O 2 ) in Brassica oleracea. Two B. oleracea genotypes with allelic differences at two QTL that result in differences in abscisic acid (ABA) signalling and seed vigour were compared. Ageing led to a similar loss in germination speed in both genotypes that was lost faster under elevated O 2 . In both genotypes, an equal oxidative shift in the glutathione redox state and a minor loss of α-tocopherol progressively occurred before seed viability was lost. In contrast, ABA levels were not affected by ageing.In conclusion, both ABA signalling and seed ageing impact on seed vigour, but not necessarily through the same biochemical mechanisms.

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Section: Discussionmentioning

confidence: 66%

Abscisic acid-determined seed vigour differences do not influence redox regulation during ageing

Schausberger

Roach

Stöggl

et al. 2019

Biochemical Journal

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“…ultra‐drying, is unpredictable (Ballesteros & Walters, ), reducing the likelihood of cumulative oxidative stress (Wang et al ., ), e.g. by vacuum sealing (Van Treuren et al ., ), storing under anoxia (Groot et al ., ) and lowering temperature (Li & Pritchard, ) appear to be promising ways of extending seed life span. Striving to achieve the maximum storage potential of crop and wild species’ seeds will be a valuable contribution to long‐term food security and plant conservation.…”

Section: Discussionmentioning

confidence: 99%

Seed life span and food security

Colville

Pritchard

2019

New Phytologist

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Summary Much is known about the inter‐specific distribution of life span in a wide diversity of vertebrates and in adult plants, but not for seeds, yet the functional trait seed life span underpins global agriculture, plant species conservation and seed persistence in the soil. We sourced data for five storage conditions (soil seed bank; high temperature – high humidity accelerated ageing; temperate, cooler, open storage; cool, dry, refrigerator; and cold, dry, freezer); and analysed the distribution of orthodox seed life span amongst crop and wild species. In all cases, whether for maximum known in situ life span in the soil seed bank (417 species), or for half‐lives (P50s) ex situ (732 species), the distribution is right‐skewed. The finding that seeds of > 50% of species are likely to have life spans ≤ 20% of the longest recorded under the same conditions has implications for future research on the evolution of seed traits and gene bank collections management.

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“…Indirect assays suggest that lipid oxidation is one of the molecular causes associated with the loss in viability (Stewart and Bewley, 1980). This process can be impeded by storing the seeds in the cold and/or anoxic instead of ambient conditions (Harrington, 1963;Groot et al, 2014). We compared the seeds of five wheat accessions harvested in 1998 and stored until 2013 in either ambient or cold conditions.…”

Section: Automatized Ms/ms Validation and Acyl Structure Annotationmentioning

confidence: 99%

Structure Annotation and Quantification of Wheat Seed Oxidized Lipids by High-Resolution LC-MS/MS

2017

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Lipid oxidation is a process ubiquitous in life, but the direct and comprehensive analysis of oxidized lipids has been limited by available analytical methods. We applied high-resolution liquid chromatography-mass spectrometry (LC-MS) and tandem mass spectrometry (MS/MS) to quantify oxidized lipids (glycerides, fatty acids, phospholipids, lysophospholipids, and galactolipids) and implemented a platform-independent high-throughput-amenable analysis pipeline for the high-confidence annotation and acyl composition analysis of oxidized lipids. Lipid contents of 90 different naturally aged wheat (Triticum aestivum) seed stocks were quantified in an untargeted high-resolution LC-MS experiment, resulting in 18,556 quantitative mass-to-charge ratio features. In a posthoc liquid chromatography-tandem mass spectrometry experiment, high-resolution MS/MS spectra (5 mD accuracy) were recorded for 8,957 out of 12,080 putatively monoisotopic features of the LC-MS data set. A total of 353 nonoxidized and 559 oxidized lipids with up to four additional oxygen atoms were annotated based on the accurate mass recordings (1.5 ppm tolerance) of the LC-MS data set and filtering procedures. MS/MS spectra available for 828 of these annotations were analyzed by translating experimentally known fragmentation rules of lipids into the fragmentation of oxidized lipids. This led to the identification of 259 nonoxidized and 365 oxidized lipids by both accurate mass and MS/MS spectra and to the determination of acyl compositions for 221 nonoxidized and 295 oxidized lipids. Analysis of 15-year aged wheat seeds revealed increased lipid oxidation and hydrolysis in seeds stored in ambient versus cold conditions. Lipid oxidation is a process inevitably connected to life. Although essential for all respiring organisms, oxygen can form reactive species (ROS). The accumulation of these compounds leads to the phenomenon commonly referred to as oxidative stress in microbes and plant and animal tissues. ROS play a role in the attack against microbial pathogens by host cells (Imlay, 2013), time-dependent death of dormant seeds (Lee et al., 2010), human diseases (Coyle and Puttfarcken, 1993;Giugliano et al., 1996), in aging (Sohal and Weindruch, 1996;Sohal et al., 2002), and in signaling processes (Gilroy et al., 2016;Mignolet-Spruyt et al., 2016) Unlike oxidized fatty acids (FAs), oxidized lipids are hardly available commercially. Targeted liquid chromatography-mass spectrometry (LC-MS) analysis of oxidized lipids using authentic standards is not very common (Hui et al., 2010;Ravandi et al., 2014). Often, oxidized lipids are detected using tandem mass spectrometry (MS/MS), where the collision-induced products of precursor ions (PRs) are scanned for fragments or neutral losses (NLs) that correspond to oxidized acyl residues expected in oxidized lipids (Spickett and Pitt, 2015), and in some cases, higher resolution MS/MS spectra are provided to support the identification (Buseman et al., 2006;Vu et al., 2012).High-resolution mass spectrometry (MS) allows for ...

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Prolonging the longevity of ex situ conserved seeds by storage under anoxia

Cited by 105 publications

References 40 publications

Abscisic acid-determined seed vigour differences do not influence redox regulation during ageing

Abscisic acid-determined seed vigour differences do not influence redox regulation during ageing

Seed life span and food security

Structure Annotation and Quantification of Wheat Seed Oxidized Lipids by High-Resolution LC-MS/MS

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