Promoter analysis and immunolocalisation show that puroindoline genes are exclusively expressed in starchy endosperm cells of wheat grain

Wiley, Paul R.; Tosi, Paola; Evrard, Alexandre; Lovegrove, Alison; Jones, Huw; Shewry, Peter R.

doi:10.1007/s11103-007-9139-x

Cited by 57 publications

(37 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Maize (Galuszka et al, 2005) Aleurone Arabinoxylan Wheat (Beaugrand et al, 2005;Beaugrand, Reis, et al, 2004;Guillon et al, 2004;Lovegrove et al, 2013;Philippe et al, 2006;Philippe et al, 2007;Robert et al, 2011;Suliman et al, 2013); oat, rye ; barley Xylan Wheat (Lovegrove et al, 2013) Xyloglucan Barley (13),(14)--D-glucan Wheat Philippe et al, 2006;Robert et al, 2011;; barley Callose Wheat (Philippe et al, 2006); barley (Wilson et al, 2012) Mannan Barley Ferulic acid Wheat (Philippe et al, 2007;Robert et al, 2011) p-Coumaric acid Wheat Tranquet et al, 2009) Xylanase Wheat (Beaugrand, Cronier, et al, 2004;Beaugrand et al, 2005;Beaugrand, Reis, et al, 2004) Glycosyltransferase Wheat (Suliman et al, 2013) Oxalate oxidase Wheat ( Wheat (Van Herpen et al, 2008;Wiley et al, 2007) Oleosin / Caleosin Rice (Chen et al, 2012); oat (Heneen et al, 2008) Puroindolines Wheat (Capparelli et al, 2005) Transfer cells Arabinoxylan Wheat Lovegrove et al, 2013;Robert et al, 2011); oat, rye, barley …”

Section: Cytokinin Dehydrogenasementioning

confidence: 99%

“…In addition to immunogold and immunofluorescence labeling, several studies have been carried out using peroxidase-or alkaline phosphatase-linked detection systems (Chen, Chyan, Jiang, Chen, & Tzen, 2012;Wiley, et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Plant tissues can be used to produce prints revealing a remarkable amount of anatomical detail, even without staining, which can be useful in recording developmental changes over time. This technique has been used, for instance, to localize puroindolines in starchy endosperm cells of wheat grain (Wiley, et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Current potential and limitations of immunolabeling in cereal grain research

Vázquez-Gutiérrez

Langton

2015

Trends in Food Science & Technology

View full text Add to dashboard Cite

Immunolabeling techniques have made a valuable contribution to cereal grain research during the past decade in terms of precise localization of specific compounds. While these techniques have several limitations, such as the availability and specificity of the antibodies, immunolabeling has proven especially useful in cereal studies seeking a better understanding of grain development and characterization. According to the literature reviewed in this paper, immunolabeling techniques will continue to be a useful tool in the characterization and localization of cereal grain components.

show abstract

Section: Cytokinin Dehydrogenasementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Current potential and limitations of immunolabeling in cereal grain research

Vázquez-Gutiérrez

Langton

2015

Trends in Food Science & Technology

View full text Add to dashboard Cite

show abstract

“…Several endosperm-specific expression promoters have been isolated and analyzed from rice (Rasmussen and Donaldson, 2006), wheat (Wiley et al, 2007), maize (Russell and Fromm, 1997), and barley (Choi et al, 2003). However, few promoters with endosperm-specific expression have been examined in plant genetic transformation studies, as the transgenic plants showed unsatisfactory expression levels and patterns (Li et al, 2008;Qu et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Cloning and functional analysis of the promoter of a maize starch synthase III gene (ZmDULL1)

Jiang

Hong

et al. 2015

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. The ZmDULL1 gene encodes a starch synthase and is a determinant of the structure of endosperm starch in maize (Zea mays L.). However, little is known regarding the regulatory mechanism of the ZmDULL1 gene. In this study, we isolated and characterized the ZmDULL1 promoter (PDULL1), which is the 5' flanking region of ZmDULL1 in maize. Sequence analysis showed that several cisacting elements important for endosperm expression (GCN4_motif and AACA-element) were located within the promoter. A series of PDULL1 deletion derivatives, PDULL1-1-PDULL1-4, from the translation start code (-1676, -1216, -740, and -343) were fused to the b-glucuronidase (GUS) reporter gene. Each deletion construct was transformed into rice using the Agrobacterium-mediated method, and then GUS activity was measured in transgenic plants. The results showed that PDULL1 was an endosperm-specific promoter. Further analysis showed that the promoter sequence (-343 to -1 base pairs) was sufficient for mediating GUS gene expression in endosperm. These results indicate that the region from -343 to -1 base pairs 5469 PDULL1 is an endosperm-specific promoter ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (2): 5468-5479 (2015) of PDULL1 is valuable for transgenic rice breeding and genetic engineering studies.

show abstract

“…Molecular genetic studies of the friabilin genes in different types of wheat have suggested that PINA is a major contributor of grain softness (Chen et al 2006;Giroux et al 2000), and functions with PINB (Amoroso et al 2004). Puroindoline genes are expressed in starchy endosperm cells, and their products are developmentally accumulated in the endosperm (Digeon et al 1999;Turnbull et al 2003a;Wiley et al 2007). PINA and PINB proteins are co-localized to the starch granule surface in the wheat endosperm, possibly due to the association of positively charged friabilins with polar lipids on the surface of starch (Feiz et al 2009).…”

mentioning

confidence: 99%

Localization of transgene-derived friabilins in rice endosperm cells

Fujiwara

Suzuki

Kudo

et al. 2014

Plant Biotechnology

View full text Add to dashboard Cite

Puroindoline-a (Pina), puroindoline-b (Pinb) genes in the wheat hardness-locus region encode 15-kDa friabilin proteins, whose accumulation in the endosperm leads to grain softness texture. In wheat, the PINA and PINB friabilins are associated with starch granules in the endosperm cell, while there is no friabilin in rice. The rice endosperm structure consisting of compound starch granules is fundamentally different from that in wheat. We previously produced two different lines of transgenic rice plants with the large genomic fragment including Pina or Pinb of Aegilops tauschii. However, localization of exogenous friabilins in the rice endosperm cell still remains to be determined. In the present study, we stacked the two different transgenic rice lines. The F 4 seeds of the stacked line, in which the homozygosity of the Pina and Pinb transgenes was checked by FISH analysis, were used for histochemical analysis of the endosperm cell. Immunodetection of PINA and PINB proteins using the Durotest antibody showed that wheat-derived friabilins were localized between compound starch granules as well as between starch granules in the rice endosperm cell. This suggests that such localization of the friabilins might prevent tight interaction between the compound starch granules and between the starch granules in the rice endosperm, leading to its soft texture.

show abstract

Promoter analysis and immunolocalisation show that puroindoline genes are exclusively expressed in starchy endosperm cells of wheat grain

Cited by 57 publications

References 37 publications

Current potential and limitations of immunolabeling in cereal grain research

Current potential and limitations of immunolabeling in cereal grain research

Cloning and functional analysis of the promoter of a maize starch synthase III gene (ZmDULL1)

Localization of transgene-derived friabilins in rice endosperm cells

Contact Info

Product

Resources

About