Promoter Methylation of MGMT Gene in Serum of Patients with Esophageal Squamous Cell Carcinoma in North East India

Das, Mandakini; Sharma, Sandeep; Sekhon, Gaganpreet Singh; Saikia, Basanta; Mahanta, Jagadish; Phukan, Rup Kumar

doi:10.7314/apjcp.2014.15.22.9955

Cited by 21 publications

(13 citation statements)

References 24 publications

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“…The current results demonstrated that no correlation was found between MGMT promoter methylation and gender, cigarette smoking, or drinking behavior, which were consistent with the results of an individual study. Baumann et al [36] reported the correlation between MGMT promoter methylation and tumor differentiation among 132 patients, but other 2 studies involving 335 patients showed no correlation between MGMT promoter methylation and tumor differentiation [27,29]. The current results demonstrated that MGMT promoter methylation was not linked to tumor differentiation.…”

Section: Discussionsupporting

confidence: 53%

“…Figure 1 shows the detailed steps of the potential literature search. Based on the selection criteria for the eligible studies, eventually 17 articles were identified in this meta-analysis [21,22,[27][28][29][30][31][32][33][34][35][36][37][38][39][40][41] these studies included a total of 1,368 patients with esophageal carcinoma and 1,489 nonmalignant controls. Sixteen studies evaluated the association between MGMT promoter methylation and esophageal carcinoma risk in cancer vs. nonmalignant controls [21,22,[28][29][30][31][32][33][34][35][36][37][38][39][40][41].…”

Section: Discussionmentioning

confidence: 99%

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Clinical Significance of O-6-Methylguanine-DNA-Methyltransferase Promoter Methylation in Patients with Esophageal Carcinoma: A Systematic Meta-Analysis

Zhang

Tong²

2017

Dig Dis

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Background: The correlation between O-6-methylguanine-DNA-methyltransferase (MGMT) promoter methylation and esophageal cancer remains controversial. This study was conducted to evaluate the clinical effect of MGMT promoter methylation on esophageal carcinoma patients. Methods: A literature search was conducted in the PubMed, EMBASE, EBSCO, and Cochrane Library databases. The overall OR and corresponding 95% CI were calculated using the random-effects model. Results: Finally, 17 eligible studies were identified in this meta-analysis; these studies included a total of 1,368 patients with esophageal carcinoma and 1,489 with nonmalignant controls. MGMT promoter methylation was significantly higher in esophageal carcinoma tissue samples than in nonmalignant tissue samples (OR 3.64, p < 0.001). Promoter methylation of the MGMT gene was not associated with gender, cigarette smoking, drinking behavior, or tumor differentiation, but MGMT promoter methylation was correlated with age (≥60 vs. <60 years: OR 1.64, p = 0.028), lymph node status (positive status vs. negative status: OR 2.39, p = 0.024), and clinical stage (stages 3-4 vs. 1-2: OR 10.59, p < 0.001). Conclusions: Our findings suggest that MGMT promoter methylation may be correlated with esophageal cancer carcinogenesis and could be associated with age, lymph node status, and clinical stage.

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Section: Discussionsupporting

confidence: 53%

Section: Discussionmentioning

confidence: 99%

Clinical Significance of O-6-Methylguanine-DNA-Methyltransferase Promoter Methylation in Patients with Esophageal Carcinoma: A Systematic Meta-Analysis

Zhang

Tong²

2017

Dig Dis

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“…Nine of ten case-control studies from SEAR demonstrated a higher risk of oesophageal cancer in SLT users 30 46 47 48 49 51 52 54 56 while one study did not report any similar risk 55 . The study from EMR reported a significant positive association between SLT use and oesophageal cancer 50 while the report from EUR 53 did not find a positive association.…”

Section: Resultsmentioning

confidence: 96%

“…Oesophageal cancer: Risk of oesophageal cancer in SLT users has been evaluated in 15 studies (11 from SEAR 9 30 46 47 48 49 51 52 54 55 56 , three EUR 13 45 53 and one EMR 50 ). Only three were cohort 9 13 45 while the rest 12 were case-control studies 30 46 47 48 49 50 51 52 53 54 55 56 . Of the cohort studies, one report each from SEAR and EUR showed significant positive association between SLT use and oesophageal cancer 9 45 .…”

Section: Resultsmentioning

confidence: 99%

Relationship between type of smokeless tobacco & risk of cancer

Gupta

Sinha³

et al. 2018

Indian Journal of Medical Research

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Background & objectives:Causative linkages of smokeless tobacco (SLT) use with oral potentially malignant disorders and cancers of oral cavity, oesophagus and pancreas have been reported. Published meta-analyses have provided pooled risk estimates for major cancers caused by SLT, both on global and regional levels. This systematic review was aimed at summarizing the available studies on occurrence and mortality risk of common cancers due to various SLT products.Methods:PubMed and Google Scholar databases were systematically searched from 1985 till January 2018 for observational studies on SLT and cancer. The included studies were evaluated and data were extracted and reviewed.Results:The review included 80 studies providing 121 risk estimates for various cancers. Majority of the studies from South-East Asian Region (SEAR) and Eastern Mediterranean Region (EMR) showed a significant positive association of SLT use with oral [odds ratio (OR) ranging from 1.48 to 27.4] and oesophageal cancers (OR between 2.06 and 12.8), while studies from European Region (EUR) reported a positive association with pancreatic cancer (OR between 1.6 and 2.1). Cancer-related mortality was evaluated in a few reports with higher risk of mortality for lung (OR between 2.0 and 9.1), cervical (OR 2.0) and prostate (OR 2.1) cancers. A wide variation was noted in the association of various cancers and specific SLT products based on their nature, methods of use and inherent toxicity. The majority of chewing tobacco products displayed higher risk for oral and oesophageal cancers while the same was not observed for snus.Interpretation & conclusions:This review emphasizes on the significantly positive association of SLT use with oral and oesophageal cancers in SEAR and EMR and pancreatic cancer in EUR. Mortality estimates for SLT-associated cancers need further analysis. Risk analysis for cancers of other sites in SLT users also requires multicentric well-designed studies.

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“…In this population, the promoter hypermethylation of certain genes may contribute toward greater risk at the molecular level for EC. 3 It has been reported that exposure to different carcinogens, namely, tobacco, alcohol, and betel nut consumption or smoking are associated with increased DNA methylation at certain genes. In Southeast Asia, smoking, alcohol consumption, and chewing of betel nut with or without tobacco are the major causes of oral cancer.…”

Section: Introductionmentioning

confidence: 99%

Alteration in the expression of MGMT and RUNX3 due to non-CpG promoter methylation and their correlation with different risk factors in esophageal cancer patients

et al. 2017

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Promoter methylation reflects in the inactivation of different genes like O 6 -methylguanine-DNA methyltransferase DNA repair gene and runt-related transcription factor 3, a known tumor suppressor gene in various cancers such as esophageal cancer. The promoter methylation was evaluated for O 6 -methylguanine-DNA methyltransferase and runtrelated transcription factor 3 in CpG, CHH, and CHG context (where H is A, T, or C) by next-generation sequencing. The methylation status was correlated with quantitative messenger RNA expression. In addition, messenger RNA expression was correlated with different risk factors like tobacco, alcohol, betel nut consumption, and smoking habit. CpG methylation of O 6 -methylguanine-DNA methyltransferase promoter had a positive association in the development of esophageal cancer (p < 0.05), whereas runt-related transcription factor 3 promoter methylation showed no significant association (p = 1.0) to develop esophageal cancer. However, the non-CpG methylation, CHH, and CHG were significantly correlated with O 6 -methylguanine-DNA methyltransferase (p < 0.05) and runt-related transcription factor 3 (p < 0.05) promoters in the development of esophageal cancer. The number of cytosine converted to thymine (C→T) in O 6 -methylguanine-DNA methyltransferase promoter showed a significant correlation between cases and controls (p < 0.05), but in runt-related transcription factor 3 no such significant correlation was observed. Besides, messenger RNA expression was found to be significantly correlated with promoter hypermethylation of O 6 -methylguanine-DNA methyltransferase and runt-related transcription factor 3 in the context of CHG and CHH (p < 0.05). The CpG hypermethylation in O 6 -methylguanine-DNA methyltransferase showed positive (p < 0.05) association, whereas in runtrelated transcription factor 3, it showed contrasting negative association (p = 0.23) with their messenger RNA expression. Tobacco, betel nut consumption, and smoking habits were associated with altered messenger RNA expression of O 6 -methylguanine-DNA methyltransferase (p < 0.05) and betel nut consumption and smoking habits were associated with runt-related transcription factor 3 (p < 0.05). There was no significant association between messenger RNA expression of O 6 -methylguanine-DNA methyltransferase and runt-related transcription factor 3 with alcohol consumption (p = 0.32 and p = 0.15). In conclusion, our results suggest that an aberrant messenger RNA expression may be the outcome of

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Promoter Methylation of MGMT Gene in Serum of Patients with Esophageal Squamous Cell Carcinoma in North East India

Cited by 21 publications

References 24 publications

Clinical Significance of O-6-Methylguanine-DNA-Methyltransferase Promoter Methylation in Patients with Esophageal Carcinoma: A Systematic Meta-Analysis

Clinical Significance of O-6-Methylguanine-DNA-Methyltransferase Promoter Methylation in Patients with Esophageal Carcinoma: A Systematic Meta-Analysis

Relationship between type of smokeless tobacco & risk of cancer

Alteration in the expression of MGMT and RUNX3 due to non-CpG promoter methylation and their correlation with different risk factors in esophageal cancer patients

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