Promoter trapping in Magnaporthe grisea

Liu, Xiao-Hong; Lu, Jianping; Wang, Jiaoyu; Min, Hang; Lin, Fu‐Cheng

doi:10.1631/jzus.2006.b0028

Cited by 3 publications

(2 citation statements)

References 19 publications

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“…Hence, it can be confirmed that the brittleness of boundaries is more relevant to the precipitation of carbonitride than the concentration of Cu in the subscale layer [16]. Figure 13 shows that original control range of N for this high strength weathering steel varies from 0.01% to 0.02%, sometimes even to 0.03%.…”

Section: Net Crack Defect Profile Of High Strength Weathering Steelmentioning

confidence: 73%

Influence of V–N Microalloying on the High-Temperature Mechanical Behavior and Net Crack Defect of High Strength Weathering Steel

Qing

Wang

Dou

et al. 2015

High Temperature Materials and Processes

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The influence of V-N microalloying on the high-temperature mechanical behavior of high strength weathering steel is discussed through thermomechanical simulation experiment. The difference of tensile strength caused by variation of [%V][%N] appears after proeutectoid phase change, and the higher level of [%V] [%N] is, the stronger the tensile strength tends to be. The ductility trough apparently becomes deeper and wider with the increase of [%V] [%N]. When the level of [%V][%N] reaches to 1.7 Â 10 −3 , high strength weathering steel shows almost similar reduction of area to 0.03% Nb-containing steel in the temperature range of 800-900℃, however, the ductility trough at the lowtemperature stage is wider than that of Nb-containing steel. Moreover, the net crack defect of bloom is optimized through the stable control of N content in low range under the precondition of high strength weathering steel with sufficient strength.

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Section: Net Crack Defect Profile Of High Strength Weathering Steelmentioning

confidence: 73%

Influence of V–N Microalloying on the High-Temperature Mechanical Behavior and Net Crack Defect of High Strength Weathering Steel

Qing

Wang

Dou

et al. 2015

High Temperature Materials and Processes

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“…This method has been used to identify tissue specific and temporally regulated genes and promoters in Arabidopsis thaliana [7,27,17]. Xiao-hong and co-workers [9] used two promoter-trapping vectors pCBGFP and pEGFPHPH, to transform protoplasts of Magnaporthe grisea and established that the promoter trapping technique can be used as a tool for functional genomics analysis. Groover and co-workers [8] reported the use of gene and enhancer trap vectors (cGT-1 and cET-1 respectively) carrying the β-glucuronidase (GUS) reporter gene into the poplar genome via Agro bacterium tumefaciens transformation to identify a new class of vascular-expressed gene tagged by enhancer trap line cET-1-pop1-145.…”

Section: Gene Trapping In Usementioning

confidence: 99%

Gene Trapping: A Powerful Tool of Functional Genomics to Identify Novel Genes

Kumari¹,

Sharma²,

Kumar³

2018

biop

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Gene trapping is a type of insertional mutagenesis that disrupts gene function by the integration of a vector in the intergen eric sequences. It provides an important and unique method for studying the relationship between gene expression and function and a powerful tool to characterize novel genes and analyze their importance in biological phenomena. It is performed with gene trap vectors that simultaneously mutate and report the expression of the endogenous gene at the site of insertion. Based on the component of gene expression cassette which they exploit, trap vectors are classified as: enhancer tr ap vectors, promoter trap vectors, gene trap vectors, poly A trap vectors and secretory trap vectors. Vectors are introduced in embryonic stem cells in mice and leaves or floral parts in case of plants by electroporation or virus-mediated transformation. The transformed cells are selected on the basis of selectable markers. Insertion events are detected and the trapped lines are established. This technique has been used to identify tissue specific and temporally regulated genes in plants and mice. It proves to be a powerful tool of functional genomics.

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Bidirectional promoter trapping T-DNA for insertional mutagenesis in Verticillium dahliae

et al. 2014

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Transfer DNA (T-DNA)-based random insertional mutagenesis is a universal forward genetic approach for gene identification and cloning in many phytopathogenic fungi. In a large number of randomly selected transformants, screening for mutants with a specific phenotype is laborious, especially for pathogenicity-defective mutants. To accelerate mutant screening and gene identification, a bidirectional promoter-trapping Ti binary vector, 1300-bisGFP-hyg, was constructed and deployed in this study. More than 6000 Verticillium dahliae transformants were obtained by the mediation of Agrobacterium tumefaciens carrying the vector. One thousand randomly selected transformants were cultured on Czapek-Dox and on Czapek-Dox plus cotton root extract media plates. The cultured transformants with green fluorescent protein (GFP) expression or changes in phenotype were selected and used in virulence or promoter-trapping assays. Based on the virulence assay of 60 transformants, the pathogenicity of 17 of these mutants was compromised. Ten pathogenicity-defective mutants were found with GFP expression, and 6 with expression in Czapek-Dox plus cotton root extract media specifically. Using TAIL-PCR (thermal asymmetric interlaced polymerase chain reaction), the T-DNA insertion sites were identified in 8 GFP-expressing transformants, including 5 pathogenicity-defective mutants and 3 unaffected transformants. Promoters of 6 genes were successfully trapped using the T-DNA method in this study. The nonpathogenic transformant 24C9 was the subject of additional investigation. It displayed strong GFP expression on water agar medium supplemented with cotton root extracts and on cotton seedling stems. The results obtained by Southern blot and quantitative real-time PCR confirmed that the transcription level of VdUGPU (encoding UTP-glucose-1-phosphate uridylyltransferase) was significantly reduced owing to T-DNA insertion in the gene promoter region. These results indicate that the bidirectional promoter-trapping Ti vector, combined with induction medium that contains root exudates, can be useful for identification of pathogenicity-related and functional genes in V. dahliae.

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Promoter trapping in Magnaporthe grisea

Cited by 3 publications

References 19 publications

Influence of V–N Microalloying on the High-Temperature Mechanical Behavior and Net Crack Defect of High Strength Weathering Steel

Influence of V–N Microalloying on the High-Temperature Mechanical Behavior and Net Crack Defect of High Strength Weathering Steel

Gene Trapping: A Powerful Tool of Functional Genomics to Identify Novel Genes

Bidirectional promoter trapping T-DNA for insertional mutagenesis in Verticillium dahliae

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