Promoters with Cancer Cell-Specific Activity for Melanoma Gene Therapy

Pleshkan, V V; Alekseenko, I V; Zinovyeva, M V; Виноградова, Т. В.

doi:10.32607/20758251-2011-3-2-13-21

Cited by 7 publications

(4 citation statements)

References 82 publications

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“…Репарационные системы, которые причастны к канцерогенезу: (1) система репарации 2-х нитевых разрывов ДНК; (2) система репарации неспраренных оснований (mismatch repair); (3) система эксцизионной репарации [18].80-90 % опухолей имеют дефекты в генах системы репарации [19,24,25,26,27,28,29]. Такой механизм способствует появлению клеточных клонов, содержащих все больше и больше вновь возникающих мутаций.…”

Section: таблица 1 биологическая функция протоонкогенов и антионкогеновunclassified

Tumor biology: molecular genetics mechanisms of initiation, promotion and progression

Агеева

Golubinskaya²,

Makalish³

et al. 2022

J Educ Health Sport

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For all tumors, regardless of type and tissue, there are a number of common features. These include high proliferative activity, disturbances in the mechanisms of apoptosis and "invisibility" for the cells of the immune system. At the basis of all these disorders lies a malfunction in the interaction between signaling proteins inside the cell, due to a violation of the expression of genes responsible for their synthesis. The aim of the review is to study the molecular biological mechanisms of carcinogenesis, characteristics and features of tumor cells that affect the development of the disease, progression, drug resistance and clinical results and the possibilities of targeted therapy of oncological diseases. Material and methods. The search for literature sources was carried out using PubMed, CyberLeninka, eLibrary, Google Scholar databases; the search depth was 15 years. Results. Recent works have shown the evolution of views on the classification of tumors from the point of view of the mechanisms of damage to the processes of cellular regulation and histogenesis, as well as the development of the modern theory of carcinogenesis, ideas about the mechanisms of development of tumor diseases and the prospects of modern therapies. Conclusion. The study of molecular biological mechanisms of carcinogenesis, characteristics and features of tumor cells is not only fundamental, but also of applied importance in order to predict the development of the disease, progression, drug resistance, clinical results and the possibilities of targeted therapy.

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Section: таблица 1 биологическая функция протоонкогенов и антионкогеновunclassified

Tumor biology: molecular genetics mechanisms of initiation, promotion and progression

Агеева

Golubinskaya²,

Makalish³

et al. 2022

J Educ Health Sport

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“…Those included promoters of oncogenes; promoters of genes involved in modulating the cell cycle; and promoters of genes known to enhance cell survival, angiogenesis, cancer progression, and metastasis. Additionally, promoters that are both tumor-specific and tissue-specific have been identified that can potentially target reporter gene expression in a particular tissue type, for example, genes involved in melanin biosynthesis (Pleshkan, Alekseenko, Zinovyeva, Vinogradova, & Sverdlov, 2011). …”

Section: Promotersmentioning

confidence: 99%

Molecular-Genetic Imaging of Cancer

Minn¹,

Menezes²,

Sarkar³

et al. 2014

Advances in Cancer Research

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Molecular-genetic imaging of cancer using nonviral delivery systems has great potential for clinical application as a safe, efficient, noninvasive tool for visualization of various cellular processes including detection of cancer, and its attendant metastases. In recent years, significant effort has been expended in overcoming technical hurdles to enable clinical adoption of molecular-genetic imaging. This chapter will provide an introduction to the components of molecular-genetic imaging and recent advances on each component leading to safe, efficient clinical applications for detecting cancer. Combination with therapy, namely, generating molecular-genetic theranostic constructs, will provide further impetus for clinical translation of this promising technology.

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“…Yet some of these promoters can be used only in some specific cancer types and this fact limits their clinical applications. 1 Survivin is a member of the apoptosis inhibitors family (AIP) whose expression and activity increase in 60% to 70% of cancers but not in adult normal tissues. Recent studies suggest that overexpression of survivin is associated with resistance to chemotherapy, hormone therapy, and radiotherapy in many types of cancers.…”

Section: Introductionmentioning

confidence: 99%

“…Yet some of these promoters can be used only in some specific cancer types and this fact limits their clinical applications. 1…”

Section: Introductionmentioning

confidence: 99%

Survivin Promoter-Driven DFF40 Gene Expression Sensitizes Melanoma Cancer Cells to Chemotherapy

2021

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Downregulation of the apoptotic protein DNA fragmentation factor 40 (DFF40) is correlated with poor overall survival in some malignancies, including melanoma. In this study, DFF40 gene expression driven by survivin promoter, a tumor-specific promoter, was used to selectively induce cytotoxicity in melanoma cells. The activity and strength of survivin promoter were examined in B16F10 murine melanoma, and L929 murine normal fibroblast cell lines using enhanced green fluorescent protein reporter assay and reverse transcription polymerase chain reaction. The effect of expression of DFF40 under the control of cytomegalovirus (CMV) or survivin promoter on viability of cancerous and normal cells was determined by MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide] assay. Apoptosis induction by expression of DFF40 was evaluated using Annexin-V/propidium iodide staining. Our findings showed high activity of survivin promoter comparable to the control promoter (ie, CMV) in melanoma cells, while survivin activity in normal cells was negligible. Survivin promoter-derived DFF40 gene expression led to selective inhibition of cell viability and induction of apoptosis in cancerous cells. Low and sublethal concentrations of a chemotherapeutic drug, dacarbazine, significantly enhanced the growth inhibitory effect of DFF40 gene therapy. Combination of survivin-driven gene therapy and chemotherapy could be considered as a potential therapeutic treatment for melanoma and possibly other malignancies with similar features.

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Promoters with Cancer Cell-Specific Activity for Melanoma Gene Therapy

Cited by 7 publications

References 82 publications

Tumor biology: molecular genetics mechanisms of initiation, promotion and progression

Tumor biology: molecular genetics mechanisms of initiation, promotion and progression

Molecular-Genetic Imaging of Cancer

Survivin Promoter-Driven DFF40 Gene Expression Sensitizes Melanoma Cancer Cells to Chemotherapy

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