Promoting early neovascularization of SIS-repaired abdominal wall by controlled release of bioactive VEGF

Tang, Rui; Wang, Xin; Zhang, Hanying; Liu, Xi; Feng, Xueyi; Zhu, Xiaoqiang; Lu, Xinwu; Wu, Fei

doi:10.1039/c7ra11954b

Cited by 13 publications

(14 citation statements)

References 38 publications

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“…Also the weaker cell–ECM bonds act locally as stress concentration or local structural defects, amplifying locally the stress field and leading to lower resistance to fracture of the native tissue [ 42 , 43 ]. The Young’s modulus of dHCM and ultimate tensile strength are both higher than SIS [ 44 ] but lower than acellular amnion membrane [ 45 ]. Nevertheless, the ability to suture dHCM was also indirectly demonstrated during our decellularization process, used in our case to identify the two sides of the dHCM.…”

Section: Discussionmentioning

confidence: 99%

Decellularized Human Chorion Membrane as a Novel Biomaterial for Tissue Regeneration

et al. 2020

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Although some placenta-derived products are already used for tissue regeneration, the human chorion membrane (HCM) alone has been poorly explored. In fact, just one study uses decellularized HCM (dHCM) with native tissue architecture (i.e., without extracellular matrix (ECM) suspension creation) as a substrate for cell differentiation. The aim of this work is to fully characterize the dHCM for the presence and distribution of cell nuclei, DNA and ECM components. Moreover, mechanical properties, in vitro biological performance and in vivo biocompatibility were also studied. Our results demonstrated that the HCM was successfully decellularized and the main ECM proteins were preserved. The dHCM has two different surfaces, the reticular layer side and the trophoblast side; and is biocompatible both in vitro and in vivo. Importantly, the in vivo experiments demonstrated that on day 28 the dHCM starts to be integrated by the host tissue. Altogether, these results support the hypothesis that dHCM may be used as a biomaterial for different tissue regeneration strategies, particularly when a membrane is needed to separate tissues, organs or other biologic compartments.

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Section: Discussionmentioning

confidence: 99%

Decellularized Human Chorion Membrane as a Novel Biomaterial for Tissue Regeneration

et al. 2020

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“…Besides the preparation of PEG-peptide conjugates to improve the peptide circulation half-life in vivo and, consequently, to diminish the dose necessary for efficient administration, the application of polymer particles for peptide drug delivery can be also matter of choice [16,17,18]. Despite the fact that the works devoted to the development of efficient delivery systems of C-peptide were not discovered, several examples on preparation of encapsulated forms of insulin can be found in the current literature [19,20,21,22,23].…”

Section: Introductionmentioning

confidence: 99%

Novel Formulations of C-Peptide with Long-Acting Therapeutic Potential for Treatment of Diabetic Complications

et al. 2019

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The development and application of novel nanospheres based on cationic and anionic random amphiphilic polypeptides with prolonged stability were proposed. The random copolymers, e.g., poly(l-lysine-co-d-phenylalanine) (P(Lys-co-dPhe)) and poly(l-glutamic acid-co-d-phenylalanine) (P(Glu-co-dPhe)), with different amount of hydrophilic and hydrophobic monomers were synthesized. The polypeptides obtained were able to self-assemble into nanospheres. Such characteristics as size, PDI and ζ-potential of the nanospheres were determined, as well as their dependence on pH was also studied. Additionally, the investigation of their biodegradability and cytotoxicity was performed. The prolonged stability of nanospheres was achieved via introduction of d-amino acids into the polypeptide structure. The cytotoxicity of nanospheres obtained was tested using HEK-293 cells. It was proved that no cytotoxicity up to the concentration of 500 µg/mL was observed. C-peptide delivery systems were realized in two ways: (1) peptide immobilization on the surface of P(Glu-co-dPhe) nanospheres; and (2) peptide encapsulation into P(Lys-co-dPhe) systems. The immobilization capacity and the dependence of C-peptide encapsulation efficiency, as well as maximal loading capacity, on initial drug concentration was studied. The kinetic of drug release was studied at model physiological conditions. Novel formulations of a long-acting C-peptide exhibited their effect ex vivo by increasing activity of erythrocyte Na+/K+-adenosine triphosphatase.

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“…Micro-nano particle materials include natural and synthetic materials such as collagen, dextran, chitosan, gelatin, silica, PLGA, etc. [56][57][58][59][60][61][62][63][64][65] Natural biomaterials usually have good biocompatibility, hydrophilicity, and biodegradability. Biomaterials with good biocompatibility can be readily recognized and tolerated by native body.…”

Section: Micro-nano Particle Embedding Methodsmentioning

confidence: 99%

“… 66 Growth factors are usually water-soluble and they can be encapsulated into the hydrophilic biomaterial micro-nanoparticles by aqueous method without any contact with the water/oil interface, organic solvents or polymers to avoid loss of bioactivity. 62 Biodegradability meets the requirements of scaffold degradation with new tissue formation. Therefore natural biomaterials can be used to prepare micro-nano particle for delivering factors.…”

Section: Loading Methods Of Growth Factors On Aliphatic Polyester Sca...mentioning

confidence: 99%

“…Similarly, in Tang's study, to release VEGF in a sustained manner with the degradation of PLGA and maintain its bioactivity concurrently, dextran nanoparticles (DNPs) loaded with vascular endothelial growth factor 165 (VEGF165) were pre-formulated by dual-aqueous phase separation method and then electrospun into the PLGA polymer fiber membrane. 62 The prepared VEGF/DNPs-PLGA membrane was sandwiched by dual-layer SIS to construct a SIS-DNPs/VEGF-PLGA-SIS (SVDPS) composite scaffold, which significantly promoted early therapeutic neovascularization within 2 weeks post-surgery.…”

Section: Loading Methods Of Growth Factors On Aliphatic Polyester Sca...mentioning

confidence: 99%

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Growth factor loading on aliphatic polyester scaffolds

Shen

2021

RSC Adv.

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Promoting early neovascularization of SIS-repaired abdominal wall by controlled release of bioactive VEGF

Abstract: Insufficient early neovascularization post-operation is thought to be the main reason of surgical recurrence of porcine small intestinal submucosa (SIS)-repaired abdominal wall defects.

Cited by 13 publications

References 38 publications

Decellularized Human Chorion Membrane as a Novel Biomaterial for Tissue Regeneration

Decellularized Human Chorion Membrane as a Novel Biomaterial for Tissue Regeneration

Novel Formulations of C-Peptide with Long-Acting Therapeutic Potential for Treatment of Diabetic Complications

Growth factor loading on aliphatic polyester scaffolds

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