Promoting effect of 5-azacytidine on the myogenic differentiation of bone marrow stromal cells

Burlacu, Alexandrina; Rosca, Ana‐Maria; Maniu, Horia; Titorencu, Irina; Dragan, Emanuel; Jinga, V.; Simionescu, Maya

doi:10.1016/j.ejcb.2007.09.003

Cited by 52 publications

(43 citation statements)

References 28 publications

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“…A variety of protocols regarding MSC differentiation into cardiomyocytes have been described, such as stimulus using demethylating agents 2,6,[10][11][12] , pool of growth factors 13 , co-culture with normal cardiomyocytes 7 , and specific gene insertions 4 . Despite satisfactory results, the in vitro differentiation into functional cardiomyocytes remains questionable.…”

Section: Introductionmentioning

confidence: 99%

Diferenciação de células-tronco mesenquimais derivadas do tecido adiposo em cardiomiócitos

Carvalho¹,

Daibert²,

Monteiro³

et al. 2013

Arq. Bras. Cardiol.

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Section: Introductionmentioning

confidence: 99%

Diferenciação de células-tronco mesenquimais derivadas do tecido adiposo em cardiomiócitos

Carvalho¹,

Daibert²,

Monteiro³

et al. 2013

Arq. Bras. Cardiol.

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“…5-azacytidine, a demethylating agent, has been reported to induce BMMSCs to differentiate into various myogenic cell types including cardiomyocytes and SkM [24]. However, the ability of 5-azacytadine to induce different myogenic lineages in MSCs may vary depending on the source of these progenitors, since in another report, only BMMSCs but not umbilical cord-or cord blood-derived MSCs can be induced by this agent into cardiomyocytes [63].…”

Section: Discussionmentioning

confidence: 99%

“…We therefore explored the myogenic differentiation capacity of these progenitor cells. It has been reported that low levels of horse serum or 5-azacytidine has the ability to promote BMMSC differentiation into myogenic lineages including cardiomyocytes and SkM [24,31]. To examine whether the hDEMSCs could differentiate into a cardiomyocytic lineage, we cultured these cells in MM without or with 5-azacytodine supplementation (MM1 and MM2, respectively), assessing first for expression of the cardiac-specific genes cardiac troponin T (TNT) and connexin-43.…”

Section: Hde-mscs Poorly Differentiate Toward a Cardiomyocytic Phenotypementioning

confidence: 99%

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Age-Related Decreases of Serum-Response Factor Levels in Human Mesenchymal Stem Cells Are Involved in Skeletal Muscle Differentiation and Engraftment Capacity

Ting

Yen

2014

Stem Cells and Development

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Skeletal muscle (SkM) comprise *40% of human body weight. Injury or damage to this important tissue can result in physical disability, and in severe cases is difficult for its endogenous stem cell-the satellite cellto reverse effectively. Mesenchymal stem cells (MSC) are postnatal progenitor/stem cells that possess multilineage mesodermal differentiation capacity, including toward SkM. Adult bone marrow (BM) is the beststudied source of MSCs; however, aging also decreases BMMSC numbers and can adversely affect differentiation capacity. Therefore, we asked whether human sources of developmentally early stage mesenchymal stem cells (hDE-MSCs) isolated from embryonic stem cells, fetal bone, and term placenta could be cellular sources for SkM repair. Under standard muscle-inducing conditions, hDE-MPCs differentiate toward a SkM lineage rather than cardiomyocytic or smooth muscle lineages, as evidenced by increased expression of SkM-associated markers and in vitro myotube formation. In vivo transplantation revealed that SkM-differentiated hDE-MSCs can efficiently incorporate into host SkM tissue in a mouse model of SkM injury. In contrast, adult BMMSCs do not express SkM-associated genes after in vitro SkM differentiation nor engraft in vivo. Further investigation of possible factors responsible for this difference in SkM differentiation potential revealed that, compared with adult BMMSCs, hDE-MSCs expressed higher levels of serum response factor (SRF), a transcription factor critical for SkM lineage commitment. Moreover, knockdown of SRF in hDE-MSCs resulted in decreased expression of SkM-related genes after in vitro differentiation and decreased in vivo engraftment. Our results implicate SRF as a key factor in age-related SkM differentiation capacity of MSCs, and demonstrate that hDEMSCs are possible candidates for SkM repair.

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“…Seifert et al (2008) did not write down significant toxic ALP changes in subjects who ingested 20 grams/day of ribose with serial measurements by day 14. Burlacu et al (2008) investigated the extent of conversion in an attempt to increase the myogenic commitment of bone marrow stromal cells (BMSC) isolated from the adult rat tibia. The results revealed that cultured cells lost the native expression of ALP with time.…”

Section: Discussionmentioning

confidence: 99%

The effect of the antioxidant drug “U-74389G” on alkaline phosphatase levels during ischemia reperfusion injury in rats

TSOMPOS¹,

PANOULİS²,

TOUTOUZAS³

et al. 2014

jecm

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Article HistoryReceived 16 / 03 / 2014 Accepted 10 / 05 / 2014The aim of this experimental study was to examine the effect of the antioxidant drug "U-74389G", on rat model and particularly in a liver ischemia reperfusion (IR) protocol. The beneficial effect or non-effectiveness of that molecule were studied biochemically using mean blood alkaline phosphatase levels (ALP). Forty rats of mean weight 231.875 gr were used in the study. ALP levels were measured 60 min after reperfusion (groups A and C) and 120 min after reperfusion (groups B and D) with administration of the drug U-74389G in groups C and D. U-74389G administration non-significantly increased the ALP levels by 19.76%±15.06% (p=0.1784), but by 31.91%±7.69% (p=0.0001) for predicted values. Reperfusion time non-significantly decreased the ALP levels by 25.21%±14.83% (p=0.0675), also by 6.38%±9.30% (p=0.3650) for predicted values. However, U-74389G administration and reperfusion time together non-significantly increased the ALP levels by 8.08%±9.19% (p=0.3696), but also by 17.75%±4.79% (p=0.0005) for predicted values. This study indicates that U-74389G administration and its interaction with reperfusion time non-significantly increased short-term effects on ALP levels. However, the predicted ALP values adjusted for rats weight, kept significantly increased ones, which stand for the restorating effects of U-74389G, that are slower than it seems.

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Promoting effect of 5-azacytidine on the myogenic differentiation of bone marrow stromal cells

Cited by 52 publications

References 28 publications

Diferenciação de células-tronco mesenquimais derivadas do tecido adiposo em cardiomiócitos

Diferenciação de células-tronco mesenquimais derivadas do tecido adiposo em cardiomiócitos

Age-Related Decreases of Serum-Response Factor Levels in Human Mesenchymal Stem Cells Are Involved in Skeletal Muscle Differentiation and Engraftment Capacity

The effect of the antioxidant drug “U-74389G” on alkaline phosphatase levels during ischemia reperfusion injury in rats

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