Promoting the activation of T cells with glycopolymer-modified dendritic cells by enhancing cell interactions

Abstract

Dendritic cell (DC) modification to enhance antigen presentation is a valuable strategy in cancer immune therapy. Other than focusing on regulating interactions between DC and antigens, we intend to promote cell interactions between DC and T cell by cell surface engineering. T cell activation is greatly improved and generates higher tumor toxicity with the aid of the synthetic glycopolymer modified on the DC surface, although the glycopolymer alone shows no effect. The great promotion of DC–T cell attraction is revealed by cell image tracking in terms of both frequency and duration of contacts. Our findings provide a new method of T cell activation by these engineered “sweet DCs.” This strategy is beneficial for developing more efficient DC-based vaccines.

Ultrahigh Efficiency and Minimalist Intracellular Delivery of Macromolecules Mediated by Latent-Photothermal Surfaces

“…pMAG or pMAM was then grafted on the cell membranes by glycopolymers-chloroalkane, as previously reported. 32,34 The cells were observed by fluorescence microscopy, and images of randomly chosen fields were captured.…”

“…Compared with the control group, the expression of the intracellular nerve cell markers, β3-tubulin and the nestin gene, increased 76-fold and 7-fold, respectively (Figure 5B), and the purity of the neurofilaments increased significantly (Figure 5C). The MWNTs/PDMS delivery device can also be exploited for the development of new therapies such as dendritic cell (DC) vaccines, 31,32 which activate T-cells to promote the body's immune response, providing new possibilities for cancer treatment. 33 We showed that pDNA expressing HaloTag protein (pHTP) could be delivered into DC cells using the MWNTs/PDMS device; poly(N-methacryloylglucosamine) (pMAG) and poly(N-methacryloylmannosamine) (pMAM) could then be grafted to the cell membrane by glycopolymerschloroalkane (Figure 5D).…”

Cell-based biocomposite engineering directed by polymers

“…26 For another example, cell surface engineering of glycopolymers promoted the contacts between different cell types through carbohydrate–lectin binding. 215,216 In this way, glycopolymer-engineered cancer cells induced a strong stimulation to M1-type macrophages, and glycopolymer-engineered dendritic cells also activated T cells much more efficiently. 215,216 Accordingly, such a glycopolymer-based cell engineering strategy displayed great potential in cancer immunotherapy.…”

“…215,216 In this way, glycopolymer-engineered cancer cells induced a strong stimulation to M1-type macrophages, and glycopolymer-engineered dendritic cells also activated T cells much more efficiently. 215,216 Accordingly, such a glycopolymer-based cell engineering strategy displayed great potential in cancer immunotherapy. Moreover, the DNA coatings on different cells were used to control cell–cell interactions by virtue of the sequence-dependent DNA hybridization.…”

The Applications of Metabolic Glycoengineering

“…Dendritic cell (DC) modification for enhancement of antigen presentation has emerged as a highly successful strategy in tumor immune therapy. Previous studies demonstrated that synthetic glycopolymer modified on the DC surface through facilitating DC interaction with T cells markedly elevated T cell activation and results in higher tumor toxicity ( Yu et al, 2020 ).…”