Promotion of bone formation using highly pure porous β-TCP combined with bone marrow-derived osteoprogenitor cells

Dong, Jian; Uemura, Toshimasa; Shirasaki, Yoshio; Tateishi, Tetsuya

doi:10.1016/s0142-9612(02)00193-x

Cited by 212 publications

(170 citation statements)

References 34 publications

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“…As may be seen, macropores in the range 100-500 lm account for most of the pore area, similarly manner to what occurs in human trabecular bone. It is agreed [21][22][23][24][25] that 100 lm is the lower size limit at which macropores enable a scaffold to provide cell migration and vascularisation; thus it may be said that the proposed scaffolds meet this requirement. A compressive strength of 7 ± 0.6 MPa was determined for the sample cubes.…”

Section: Resultsmentioning

confidence: 99%

Biocompatible glass–ceramic materials for bone substitution

Brovarone

Verné

Robiglio

et al. 2007

J Mater Sci: Mater Med

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A new bioactive glass composition (CEL2) in the SiO 2 -P 2 O 5 -CaO-MgO-K 2 O-Na 2 O system was tailored to control pH variations due to ion leaching phenomena when the glass is in contact with physiological fluids. CEL2 was prepared by a traditional meltingquenching process obtaining slices that were heat-treated to obtain a glass-ceramic material (CEL2GC) that was characterized thorough SEM analysis. Pre-treatment of CEL2GC with SBF was found to enhance its biocompatibility, as assessed by in vitro tests. CEL2 powder was then used to synthesize macroporous glass-ceramic scaffolds. To this end, CEL2 powders were mixed with polyethylene particles within the 300-600 lm size-range and then pressed to obtain crack-free compacted powders (green). This was heat-treated to remove the organic phase and to sinter the inorganic phase, leaving a porous structure. The biomaterial thus obtained was characterized by X-ray diffraction, SEM equipped with EDS, density measurement, image analysis, mechanical testing and in vitro evaluation, and found to be a glass-ceramic macroporous scaffold with uniformly distributed and highly interconnected porosity. The extent and size-range of the porosity can be tailored by varying the amount and size of the polyethylene particles.

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Section: Resultsmentioning

confidence: 99%

Biocompatible glass–ceramic materials for bone substitution

Brovarone

Verné

Robiglio

et al. 2007

J Mater Sci: Mater Med

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“…For example, the cells' adhesive, proliferation, and differentiation processes may be adversely affected by exposure of the cells to air, humidity, and temperature changes and various forms of radiation. [2][3][4][5] If possible, rapidly infusing cells into implantable scaffolds would reduce their exposure to these environmental changes, potentially improving the quality of the tissue engineered from the cells. Further, reducing the seeding time makes it possible to perform this task at the point of care, i.e., in the surgical theater, for example.…”

Section: Introductionmentioning

confidence: 99%

“…Using other techniques, cells are merely deposited on the surfaces of scaffolds. [2][3][4][5][6] As a result, the distribution of the new cultured tissue in the scaffold is not uniform and the tissue repair process is consequently delayed after implantation. 4,6 Rapid and uniform cell seeding-while maintaining cell viability and functionality-is the paragon for enabling tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

Effect of surface acoustic waves on the viability, proliferation and differentiation of primary osteoblast-like cells

Li¹,

Friend²,

Yeo³

et al. 2009

Biomicrofluidics

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Surface acoustic waves ͑SAWs͒ have been used as a rapid and efficient technique for driving microparticles into a three-dimensional scaffold matrix, raising the possibility that SAW may be effective in seeding live cells into scaffolds, that is, if the cells were able to survive the infusion process. Primary osteoblast-like cells were used to specifically address this issue: To investigate the effects of SAW on the cells' viability, proliferation, and differentiation. Fluorescence-labeled osteoblastlike cells were seeded into polycaprolactone scaffolds using the SAW method with a static method as a control. The cell distribution in the scaffold was assessed through image analysis. The cells were far more uniformly driven into the scaffold with the SAW method compared to the control, and the seeding process with SAW was also significantly faster: Cells were delivered into the scaffold in seconds compared to the hour-long process of static seeding. Over 80% of the osteoblastlike cells were found to be viable after being treated with SAW at 20 MHz for 10-30 s with an applied power of 380 mW over a wide range of cell suspension volumes ͑10-100 ᐉ͒ and cell densities ͑1000-8000 cells/ ᐉ͒. After determining the optimal cell seeding parameters, we further found that the treated cells offered the same functionality as untreated cells. Taken together, these results show that the SAW method has significant potential as a practical scaffold cell seeding method for tissue and orthopedic engineering.

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“…A tissue engineering approach combining an osteoconductive biomaterial scaffold and bone marrow mesenchymal stem cells has also been examined to heal or augment bone regeneration [10, 15,16,17,19,38,39]. This study examined the effects of autologous biological therapy through isolation of multiple growth factors using a twostage buffy coat (BC) sequestration protocol followed by concentration using an ultrafiltration device.…”

Section: Introductionmentioning

confidence: 99%