Promotion of natural tooth repair by small molecule GSK3 antagonists

Abstract

The restoration of dentine lost in deep caries lesions in teeth is a routine and common treatment that involves the use of inorganic cements based on calcium or silicon-based mineral aggregates. Such cements remain in the tooth and fail to degrade and thus normal mineral volume is never completely restored. Here we describe a novel, biological approach to dentine restoration that stimulates the natural formation of reparative dentine via the mobilisation of resident stem cells in the tooth pulp. Biodegradable, clinically-approved collagen sponges are used to deliver low doses of small molecule glycogen synthase kinase (GSK-3) antagonists that promote the natural processes of reparative dentine formation to completely restore dentine. Since the carrier sponge is degraded over time, dentine replaces the degraded sponge leading to a complete, effective natural repair. This simple, rapid natural tooth repair process could thus potentially provide a new approach to clinical tooth restoration.

Use of 0.4‐Tesla static magnetic field to promote reparative dentine formation of dental pulp stem cells through activation of p38 MAPK signalling pathway

“…Severe tooth trauma caused by deep caries removal or tooth preparation may result in necrosis of the dental pulp tissue if the natural reparative dentine formation is insufficient to effect a proper repair (Neves et al . ). The present results have shown that the application of 0.4‐T static magnetic field promotes DPSC proliferation, migration and dentinogenic differentiation through the activation of the p38 MAPK signalling pathway.…”

“…Neves et al . () applied three different glycogen synthase kinase 3 (GSK3) inhibitors to exposed animal dental pulp and found that all three antagonist groups exhibited a more obvious reparative dentine formation tendency than the sham control group or a mineral trioxide aggregate (MTA) group. Several studies have shown that the dentinogenic behaviour of DPSCs is affected by the mechanical properties of the extracellular microenvironment.…”

Inibidores Da GSK-3: Uma Nova Estratégia Para a Regeneração Dental

“…Em um estudo coordenado por Neves et al (2017), foram testados inibidores da molécula de GSK-3, BIO (2'Z, 3'E)-6-bromoindirubina-3'-oxima),…”

“…No procedimento experimental, realizado por Neves et al (2017), a perfuração foi realizada nos dois primeiros molares superiores dos animais e as esponjas foram inseridas e deixadas por 4-6 semanas antes da remoção. Para proteger a polpa da contaminação externa e estimular o reparo da dentina, a lesão foi oclusa com o agregado de trióxido de minério (MTA).…”

“…Os autores enfatizam que a esponja de colágeno utilizada como veículo dos fármacos é degradada ao longo do tempo, sendo substituída por uma nova dentina, levando ao reparo natural, completo e efetivo. Este processo rápido e simples de reparação dos dentes pode fornecer uma nova abordagem para a restauração na clínica dentária (NEVES et al, 2017).…”