Promotion of pro‐osteogenic responses by a bioactive ceramic coating

Abstract

The objective of this study was to analyze the responses of bone-forming osteoblasts to Ti-6Al-4V implant material coated with silica-calcium phosphate nanocomposite (SCPC50). Osteoblast differentiation at the interface with SCPC50-coated Ti-6Al-4V was correlated to the adsorption of high amount of serum proteins, high surface affinity to fibronectin, Ca uptake from and P and Si release into the medium. SCPC50-coated Ti-6Al-4V adsorbed significantly more serum protein (p < 0.05) than control uncoated substrates. Moreover, Western blot analysis showed that the SCPC50 coating had a high affinity for serum fibronectin. Protein conformation analyses by FTIR showed that the ratio of the area under the peak for amide I/amide II bands was significantly higher (p < 0.05) on the surface of SCPC50-coated substrates than that on the surface of the control uncoated substrates. Moreover, ICP À OES analyses indicated that SCPC50-coated substrates withdrew Ca ions from, and released P and Si ions into, the tissue culture medium, respectively. In conjunction with the favorable protein adsorption and modifications in medium composition, MC3T3-E1 osteoblast-like cells attached to SCPC50coated substrates expressed 10-fold higher level of mRNA encoding osteocalcin and had significantly higher production of osteopontin and osteocalcin proteins than cells attached to the uncoated Ti-6A1-4V substrates. In addition, osteoblast-like cells attached to the SCPC50-coated substrates produced significantly lower levels of the inflammatory and osteoclastogenic cytokines, IL-6, IL-12p40, and RANKL than those attached to uncoated Ti-6Al-4V substrates. These results suggest that SCPC50 coating could enhance bone integration with orthopedic and maxillofacial implants while minimizing the induction of inflammatory bone cell responses. V

Early osteoblast responses to orthopedic implants: Synergy of surface roughness and chemistry of bioactive ceramic coating

“…However, bioceramic dissolution in presence and absence of cells is not analogous, in fact, the dissolution-precipitation reactions and ECM mineralization are strongly cell-mediated. 12,13 The adsorption of proteins and biological macromolecules is considered to be the first stage during implant integration, followed by cell attachment. 14 Protein adsorption on biomaterials, however, is not an "across-the-board" phenomenon; the surface chemistry of the material controls the species and conformation of proteins that it adsorbs.…”

“…21 In vitro studies have shown that SCPC50 coating stimulated biological hydroxyapatite formation, enhanced osteoblast differentiation, and induced a low level immunogenic response as compared to the cells attached to uncoated Ti-6Al-4V substrates. 13,21,22 The objective of the present study is to investigate the synergistic effects of surface roughness and material chemistry of SCPC50 coating on modulating key early cellular functions leading to osteoblast differentiation on orthopedic implants. Temporal variations in cell morphology and cytoskeletal organization have been analyzed and correlated to the topography and ionic dissolution of the bioactive ceramic.…”

Acceleration of Alveolar Ridge Augmentation Using a Low Dose of Recombinant Human Bone Morphogenetic Protein-2 Loaded on a Resorbable Bioactive Ceramic

“…In conjunction with the absorption of high Ca concentration, the attached stem cells produced calcified nodules and mineralized extracellular matrix (27,28) . On the mRNA level, qRT-PCR revealed that osteopontin and osteocalcin mRNA expression by osteoblast-like cells attached to SCPC50 was significantly higher than that on SCPC10, HA-200 or polystyrene after 2 days in culture (30)(31)(32)(33) .…”

Synthesis and characterization of a novel scaffold for bone tissue engineering based on Wharton's jelly

“…Silica, in turn, plays an important role in bone marrow cell differentiation, increasing the phenotypic expression of osteoblasts . In addition, it has been demonstrated that this type of implant coating induces higher protein adsorption, enhancing bone integration while minimizing the induction of inflammatory responses …”

“…19 In addition, it has been demonstrated that this type of implant coating induces higher protein adsorption, enhancing bone integration while minimizing the induction of inflammatory responses. 20 The aforementioned issues prompted the present work, in which a novel use of WJ as a three-dimensional matrix in combination with BC (HA and btricalcium phosphate, b-TCP) and BG particles, is proposed. This kind of combination would generate a synergistic effect resulting in a bone-like tissue, enhancing the biological and mechanical behavior of BCO.…”