Promotive effect of polyvinylpolypyrrolidone on pollen embryogenesis in Datura innoxia

Tyagi, Akhilesh K.; Rashid, Abdur; Maheshwari, S. C.

doi:10.1111/j.1399-3054.1981.tb02722.x

Cited by 21 publications

(6 citation statements)

References 8 publications

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“…The initial inhibition may be caused by adsorbtion of some conditioning substance, released by the anthers, as suggested by Johansson and Eriksson (1984). The stimulation of embryogenesis during the last weeks of incubation is probably caused by adsorbtion of phenolic substances, as suggested by Tyagi et al (1981). It is also worth observing that PVPP, bound in solid medium severely inhibited embryogenesis, both alone and especially in combination with AC.…”

Section: Discussionmentioning

confidence: 81%

Correlations between activated charcoal, Fe‐EDTA and other organic media ingredients in cultured anthers of Anemone canadensis

Johansson

Calleberg

Gedin

1990

Physiologia Plantarum

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The effects of activated charcoal and organic substances on embryogenesis in anther cultures of Anemone canadensis L. were studied. Embryogenesis was independent of the presence in the culture medium of glycine, nicotinic acid, pyridoxine, thiamine, folic acid, d‐biotin or myoinositol. Absence of Fe‐EDTA totally inhibited embryogenesis. Activated charcoal (AC) adsorbed Fe‐EDTA, pyridoxine, folic acid and nicotinic acid in a double‐layer medium almost completely within 24 h. If petri dishes according to the souble‐layer method were stored overnight or more, embryogenesis was totally inhibited, probably due to adsorbtion of Fe‐EDTA by AC. It was shown that AC itself released some yet unidentified substance(s) that stimulated embryogenesis. The addition of polyvinylpolypyrrolidone (PVPP) to the culture medium stimulated embryogenesis, but PVPP was not as efficient as AC. Embryogenesis was totally inhibited when AC and PVPP were applied together. Minor additions of ethanol to the culture medium stimulated embryogenesis when AC was present, but no such effect was obtained when AC was absent.

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Section: Discussionmentioning

confidence: 81%

Correlations between activated charcoal, Fe‐EDTA and other organic media ingredients in cultured anthers of Anemone canadensis

Johansson

Calleberg

Gedin

1990

Physiologia Plantarum

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“…Embryo production was improved by the addition of PVP in the culture medium that also decreased anther browning. Polyvinylpolypyrrolidone, an insoluble high molecular weight, cross-linked polymer of PVP, had a promotive effect on anther culture of Datura (Tyagi et al 1981) and browning has been shown as detrimental for embryogenesis in rubber tree callus (Housti. et al 1991).…”

Section: Resultsmentioning

confidence: 99%

Assay for doubled haploid sunflower (Helianthus annuus) plant production by androgenesis: fact or artifact? Part 1. In vitro anther culture

Zhong

Michaux-Ferrière

Coumans

1995

Plant Cell Tiss Organ Cult

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Sunflower anthers placed on solid medium developed calli and embryos after 12 days. Embryogenesis was improved by the addition of 0.1% polyvinylpyrrolidone (PVP) that alleviated anther and medium browning. As in other species, genotypic variability was an important parameter in the anther response and a medium genotype interaction was suggested with a different PVP effect depending on the genotype. Embryo germination was largely increased by the successive use of germination media with decreasing sucrose concentrations (10% ffi> 6% ffi> 3 %). Histological examination of the anthers during the first ten days of culture showed that, under our conditions, the embryos were of somatic origin, arising directly from the anther wall on the outside or inside of the anther loculus, or indirectly from proliferating anther wall-or connective tissue-derived callus. Finally, the ploidy status of 78 embryo-derived plants was determined by Feulgen stain or flow cytometry : all plants were diploid (2n -34).Abbreviations: PVP -polyvinylpyrrolidone

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“…According to these results, Zhong et al (1995) alleviated anther and medium browning in anther culture of sunflower by the addition of 0.1% PVP in culture medium. PVP is insoluble with high molecular weight and has also shown promotive effects on anther culture of Datura (Tyagi et al, 1981).…”

Section: Discussionmentioning

confidence: 99%

Improvement in androgenic response of borage (Borago officinalis L.) cultured anthers using antibrowning agents and picloram

Abdollahi¹,

Eshaghi²,

Majdi³

2017

Turk J Biol

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Given the importance of borage (Borago officinalis L.) as a medicinal herb, androgenic studies are necessary in order to speed up the breeding programs of this plant species. In this work, we evaluated the effect of silver nitrate, activated charcoal, ascorbic acid, and polyvinylpyrrolidone (PVP), previously described as antibrowning agents and promoters of androgenesis. Adding silver nitrate at a concentration of 5 mg L -1 significantly enhanced the androgenic responses of cultured anthers up to ~2-fold for total calli production (53.33%) and up to ~2.5-fold for embryonic calli and embryo formation (30% and 0.3 embryos/anther, respectively) compared to control culture medium. Culture media containing 5 and 10 mg L -1 silver nitrate and 2 mg L -1 PVP significantly reduced the callus browning and led to increased callus viability in borage cultured anthers. We also evaluated the effect of various combinations of picloram with 2-isopentyl adenine and 2,4-dichlorophenoxyacetic acid (2,4-D) as growth regulators in the standard protocol. Significantly better results were obtained by adding 2 mg L -1 picloram in combination with 2 mg L -1 2,4-D in culture medium. This medium produced the highest frequencies of callogenesis (48.33%), embryogenic calli (20%), and embryo formation (0.2 embryos/anther) compared to control and other growth regulator combinations.

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Promotive effect of polyvinylpolypyrrolidone on pollen embryogenesis in Datura innoxia

Cited by 21 publications

References 8 publications

Correlations between activated charcoal, Fe‐EDTA and other organic media ingredients in cultured anthers of Anemone canadensis

Correlations between activated charcoal, Fe‐EDTA and other organic media ingredients in cultured anthers of Anemone canadensis

Assay for doubled haploid sunflower (Helianthus annuus) plant production by androgenesis: fact or artifact? Part 1. In vitro anther culture

Improvement in androgenic response of borage (Borago officinalis L.) cultured anthers using antibrowning agents and picloram

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